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Kinase Gene Expression and Subcellular Protein Expression Pattern of Protein Kinase C Isoforms in Curcumin-treated Human Hepatocellular Carcinoma Hep 3B Cells

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Abstract

Curcumin, a yellow component of turmeric or curry powder, has been demonstrated to exhibit anti-carcinogenic effects in vitro, in vivo, and in human clinical trials. One of its molecular targets is protein kinase C (PKC) which has been reported to play essential roles in apoptosis, cell proliferation, and carcinogenesis. In this study, PKC mRNA expression was significantly inhibited in curcumin-treated human hepatocellular carcinoma (HCC) Hep 3B cells identified using a kinase cDNA microarray. Furthermore, curcumin decreased total protein expression of all PKCs in a time-related manner by immunoblotting of whole cell lysates, nuclear, membrane, and cytosolic fractions. In cytosolic fraction, the expression of PKC-α was totally inhibited by curcumin. In contrast, the expression levels of PKC-ζ and -μ were dramatically increased. Increases in expression of PKC-δ and PKC-ζ in the membrane and nucleus, and PKC-ι in the membrane were detected. In summary, the changes in expression and distribution of subcellular PKC isoforms in curcumin-treated Hep 3B cells suggest possible PKC-associated anti-tumor mechanisms of curcumin and provide alternative therapies for human HCC.

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Acknowledgments

This study was supported by grants from the National Science Council, Taiwan (NSC 93-2320-B-309-002 and NSC 98-2313-B-003-002-MY3), Ministry of Economic Affairs, Taiwan (99-EC-17-A-17-S1-152), and National Taiwan Normal University, Taiwan (No. 99-D).

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Authors and Affiliations

  1. Department of Food Nutrition, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, Tainan, 717, Taiwan

    Hsin-Hsin Kao

  2. Institute of Medical Research, Chang Jung Christian University, No. 396, Sec. 1, Changrong Road, Gueiren, Tainan, 711, Taiwan

    Chao-Jung Wu

  3. Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, No. 1, University Road, Tainan, 701, Taiwan

    Shen-Jeu Won, Jyh-Wei Shin & Hsiao-Sheng Liu

  4. Department of Parasitology, College of Medicine, National Cheng Kung University, No. 1, University Road, Tainan, 701, Taiwan

    Jyh-Wei Shin

  5. Department of Human Development and Family Studies, National Taiwan Normal University, No. 162, Sec. 1, He-ping East Road, Taipei, 106, Taiwan

    Chun-Li Su

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  1. Hsin-Hsin Kao
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Correspondence to Chun-Li Su.

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Kao, HH., Wu, CJ., Won, SJ. et al. Kinase Gene Expression and Subcellular Protein Expression Pattern of Protein Kinase C Isoforms in Curcumin-treated Human Hepatocellular Carcinoma Hep 3B Cells. Plant Foods Hum Nutr 66, 136–142 (2011). https://doi.org/10.1007/s11130-011-0228-2

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