Plant Foods for Human Nutrition

, Volume 66, Issue 2, pp 136–142 | Cite as

Kinase Gene Expression and Subcellular Protein Expression Pattern of Protein Kinase C Isoforms in Curcumin-treated Human Hepatocellular Carcinoma Hep 3B Cells

  • Hsin-Hsin Kao
  • Chao-Jung Wu
  • Shen-Jeu Won
  • Jyh-Wei Shin
  • Hsiao-Sheng Liu
  • Chun-Li Su
Original Paper


Curcumin, a yellow component of turmeric or curry powder, has been demonstrated to exhibit anti-carcinogenic effects in vitro, in vivo, and in human clinical trials. One of its molecular targets is protein kinase C (PKC) which has been reported to play essential roles in apoptosis, cell proliferation, and carcinogenesis. In this study, PKC mRNA expression was significantly inhibited in curcumin-treated human hepatocellular carcinoma (HCC) Hep 3B cells identified using a kinase cDNA microarray. Furthermore, curcumin decreased total protein expression of all PKCs in a time-related manner by immunoblotting of whole cell lysates, nuclear, membrane, and cytosolic fractions. In cytosolic fraction, the expression of PKC-α was totally inhibited by curcumin. In contrast, the expression levels of PKC-ζ and -μ were dramatically increased. Increases in expression of PKC-δ and PKC-ζ in the membrane and nucleus, and PKC-ι in the membrane were detected. In summary, the changes in expression and distribution of subcellular PKC isoforms in curcumin-treated Hep 3B cells suggest possible PKC-associated anti-tumor mechanisms of curcumin and provide alternative therapies for human HCC.


cDNA microarray Curcumin Hepatocellular carcinoma Isoforms Protein kinase C 



This study was supported by grants from the National Science Council, Taiwan (NSC 93-2320-B-309-002 and NSC 98-2313-B-003-002-MY3), Ministry of Economic Affairs, Taiwan (99-EC-17-A-17-S1-152), and National Taiwan Normal University, Taiwan (No. 99-D).

Supplementary material

11130_2011_228_MOESM1_ESM.pdf (43 kb)
ESM 1 (PDF 43 kb)


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Hsin-Hsin Kao
    • 1
  • Chao-Jung Wu
    • 2
  • Shen-Jeu Won
    • 3
  • Jyh-Wei Shin
    • 3
    • 4
  • Hsiao-Sheng Liu
    • 3
  • Chun-Li Su
    • 5
  1. 1.Department of Food NutritionChung Hwa University of Medical TechnologyTainanTaiwan
  2. 2.Institute of Medical ResearchChang Jung Christian UniversityTainanTaiwan
  3. 3.Department of Microbiology and Immunology, College of MedicineNational Cheng Kung UniversityTainanTaiwan
  4. 4.Department of Parasitology, College of MedicineNational Cheng Kung UniversityTainanTaiwan
  5. 5.Department of Human Development and Family StudiesNational Taiwan Normal UniversityTaipeiTaiwan

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