Plant Foods for Human Nutrition

, Volume 66, Issue 1, pp 17–21 | Cite as

Aged Garlic Extract Scavenges Superoxide Radicals

  • Naoaki Morihara
  • Minoru Hayama
  • Hirotada Fujii


There is increasing evidence to suggest that many degenerative or pathological processes, such as aging, cancer, and coronary heart disease, are related to reactive oxygen species and radical-mediated reactions. We examined the effectiveness of aged garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, and its component for scavenging of superoxide by using the hypoxanthine-xanthine oxidase and human neutrophils. In the hypoxanthine-xanthine oxidase system, electron spin resonance showed that aged garlic extract scavenged superoxide radicals in a dose-dependent manner up to 54%. The EC50 value of aged garlic extract for the superoxide radical scavenging effect was 0.80 mg/ml. N-α-(1-deoxy-D-fructos-1-yl)-L-arginine (25.9%) and (1S, 3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-1,3-dicarboxylic acid (20.8%), water-soluble moieties of AGE, also exerted superoxide scavenging effects. Phorbol 12-myristate 13-acetate-activated human neutrophils produced superoxide radical of 56.6 ± 9.27 nmol/min/107 cells. Aged garlic extract (3 mg/ml) significantly inhibited superoxide production in comparison to the control. These data suggest that aged garlic extract may be useful for preventing diseases associated with reactive oxygen species.


Aged garlic extract Electron spin resonance Free radical Neutrophil Superoxide 



aged garlic extract




diethylenetriamine-N,N,N′,N″,N″-pentaacetic acid


electron spin resonance








1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid


phorbol 12-myristate 13-acetate


reactive oxygen species






xanthine oxidase


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Naoaki Morihara
    • 1
  • Minoru Hayama
    • 1
  • Hirotada Fujii
    • 2
  1. 1.Healthcare Research InstituteWakunaga Pharmaceutical Co. Ltd.HiroshimaJapan
  2. 2.School of Health SciencesSapporo Medical UniversitySapporoJapan

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