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Plant Foods for Human Nutrition

, Volume 66, Issue 1, pp 17–21 | Cite as

Aged Garlic Extract Scavenges Superoxide Radicals

  • Naoaki Morihara
  • Minoru Hayama
  • Hirotada Fujii
ORIGINAL PAPER

Abstract

There is increasing evidence to suggest that many degenerative or pathological processes, such as aging, cancer, and coronary heart disease, are related to reactive oxygen species and radical-mediated reactions. We examined the effectiveness of aged garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, and its component for scavenging of superoxide by using the hypoxanthine-xanthine oxidase and human neutrophils. In the hypoxanthine-xanthine oxidase system, electron spin resonance showed that aged garlic extract scavenged superoxide radicals in a dose-dependent manner up to 54%. The EC50 value of aged garlic extract for the superoxide radical scavenging effect was 0.80 mg/ml. N-α-(1-deoxy-D-fructos-1-yl)-L-arginine (25.9%) and (1S, 3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-1,3-dicarboxylic acid (20.8%), water-soluble moieties of AGE, also exerted superoxide scavenging effects. Phorbol 12-myristate 13-acetate-activated human neutrophils produced superoxide radical of 56.6 ± 9.27 nmol/min/107 cells. Aged garlic extract (3 mg/ml) significantly inhibited superoxide production in comparison to the control. These data suggest that aged garlic extract may be useful for preventing diseases associated with reactive oxygen species.

Keywords

Aged garlic extract Electron spin resonance Free radical Neutrophil Superoxide 

Abbreviations

AGE

aged garlic extract

DEPMPO

5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide

DTPA

diethylenetriamine-N,N,N′,N″,N″-pentaacetic acid

ESR

electron spin resonance

Fru-Arg

N-α-(1-deoxy-D-fructos-1-yl)-L-arginine

GSAC

γ-glutamyl-S-allyl-L-cysteine

HPX

hypoxanthine

MTCC

1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid

PMA

phorbol 12-myristate 13-acetate

ROS

reactive oxygen species

SAC

S-allyl-L-cysteine

SMC

S-methyl-L-cysteine

XOD

xanthine oxidase

References

  1. 1.
    Anderson D (1999) Antioxidant defenses against reactive oxygen species causing genetic and other damage. Mutat Res 350:103–108Google Scholar
  2. 2.
    Gey KF (1990) The antioxidant hypothesis of cardiovascular disease: Epidemiology and mechanisms. Biochem Soc Trans 18:1041–1045Google Scholar
  3. 3.
    Tahirovic I, Sofic E, Sapcanin A, Gavrankapetanovic I, Bach-Rojecky L, Salkovic-Petrisic M, Lackovic Z, Hoyer S, Riederer P (2007) Brain antioxidant capacity in rat models of betacytotoxic-induced experimental sporadic Alzheimer’s disease and diabetes mellitus. J Neural Transm Suppl 72:235–240CrossRefGoogle Scholar
  4. 4.
    Janzen EG (1971) Chemistry and biology of spin-trapping radicals. Accounts Chem Res 4:31–40CrossRefGoogle Scholar
  5. 5.
    Dausch JG, Nixon DW (1990) Garlic: A review of its relationship to malignant disease. Prev Med 19:346–361CrossRefGoogle Scholar
  6. 6.
    Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y (2001) Intake of garlic and its bioactive components. J Nutr 131:955S–962SGoogle Scholar
  7. 7.
    Nakagawa S, Masamoto K, Sumiyoshi H, Kunihiro K, Fuwa T (1980) Effect of raw and extracted-aged garlic juice on growth of young rats and their organs after peroral administration. J Toxicol Sci 5:91–112Google Scholar
  8. 8.
    Morihara N, Ushijima M, Kashimoto N, Sumioka I, Nishihama T, Hayama M, Takeda H (2006) Aged garlic extract ameliorates physical fatigue. Biol Pharm Bull 29:962–966CrossRefGoogle Scholar
  9. 9.
    Morihara N, Ide N, Weiss N (2010) Aged garlic extract inhibits CD36 expression in human macrophages via modulation of the PPARγ pathway. Phytother Res 24:602–608Google Scholar
  10. 10.
    Imai J, Ide N, Nagae S, Moriguchi T, Matsuura H, Itakura Y (1994) Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Med 60:417–420CrossRefGoogle Scholar
  11. 11.
    Moriguchi T, Saito H, Nishiyama N (1996) Aged garlic extract prolongs longevity and improves spatial memory deficit in senescence-accelerated mouse. Biol Pharm Bull 19:305–307Google Scholar
  12. 12.
    Ryu K, Ide N, Matsuura H, Itakura Y (2001) N alpha-(1-deoxy-D-fructos-1-yl)-L-arginine, an antioxidant compound identified in aged garlic extract. J Nutr 131:972S–976SGoogle Scholar
  13. 13.
    Ichikawa M, Ryu K, Yoshida J, Ide N, Yoshida S, Sasaoka T, Sumi S (2002) Antioxidant effects of tetrahydro-beta-carboline derivatives identified in aged garlic extract. Biofactors 16:57–72CrossRefGoogle Scholar
  14. 14.
    United States Pharmacopeial Convention I (2005) United States Pharmacopoeia 28 Garlic Fluidextract USP 28-NF 23. United States Pharmacopeial Convention, Rockville, MD, pp 2089–2090Google Scholar
  15. 15.
    Roubaud V, Sankarapandi S, Kuppusamy P (1997) Quantitative measurement of superoxide generation using the spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide. Anal Biochem 247:404–411CrossRefGoogle Scholar
  16. 16.
    Böyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest 97:77–89Google Scholar
  17. 17.
    Babior BM, Kipnes RS, Curnutte JT (1973) Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest 52:741–744CrossRefGoogle Scholar
  18. 18.
    Harbour JR, Chow V, Bolton JR (1974) An electron spin resonance study of the spin adducts of OH and HO2 radicals with nitrones in the ultraviolet photolysis of aqueous hydrogen peroxide solutions. Can J Chem 52:3549–3553CrossRefGoogle Scholar
  19. 19.
    Shishehbor MH, Hazen SL (2004) Antioxidant studies need a change of direction. Cleve Clin J Med 71:285–288CrossRefGoogle Scholar
  20. 20.
    Bayraktutan U (2002) Free radicals, diabetes and endothelial dysfunction. Diab Obes Metab 4:224–238CrossRefGoogle Scholar
  21. 21.
    Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milzani A (2006) Biomarkers of oxidative damage in human disease. Clin Chem 52:601–623CrossRefGoogle Scholar
  22. 22.
    Frejaville C, Karoui H, Tuccio B, Le Moigne F, Culcasi M, Pietri S, Lauricella R, Tordo P (1995) 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide: A new efficient phosphorylated nitrone for the in vitro and in vivo spin trapping of oxygen-centered radicals. J Med Chem 38:258–265CrossRefGoogle Scholar
  23. 23.
    Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239–247CrossRefGoogle Scholar
  24. 24.
    Giugliano D, Ceriello A, Paolisso G (1995) Diabetes mellitus, hypertension, and cardiovascular disease: Which role for oxidative stress? Metabolism 44:363–368CrossRefGoogle Scholar
  25. 25.
    Bjerrum OW, Borregaard N (1989) Dual granule localization of the dormant NADPH oxidase and cytochrome b559 in human neutrophils. Eur J Haematol 43:67–77CrossRefGoogle Scholar
  26. 26.
    Jesaitis AJ, Buescher ES, Harrison D, Quinn MT, Parkos CA, Livesey S, Linner J (1990) Ultrastructural localization of cytochrome b in the membranes of resting and phagocytosing human granulocytes. J Clin Invest 85:821–835CrossRefGoogle Scholar
  27. 27.
    Saravanan G, Ponmurugan P (2010) Beneficial effect of S-allylcysteine (SAC) on blood glucose and pancreatic antioxidant system in streptozotocin diabetic rats. Plant Foods Hum Nutr 65:374–378CrossRefGoogle Scholar
  28. 28.
    Park JH, Park YK, Park E (2009) Antioxidative and antigenotoxic effects of garlic (Allium sativum L.) prepared by different processing methods. Plant Foods Hum Nutr 64:244–249CrossRefGoogle Scholar
  29. 29.
    Ceriello A (2003) New insights on oxidative stress and diabetic complications may lead to a “causal” antioxidant therapy. Diab Care 26:1589–1596CrossRefGoogle Scholar
  30. 30.
    Vasdev S, Gill VD, Singal PK (2006) Modulation of oxidative stress-induced changes in hypertension and atherosclerosis by antioxidants. Exp Clin Cardiol 11:206–216Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Naoaki Morihara
    • 1
  • Minoru Hayama
    • 1
  • Hirotada Fujii
    • 2
  1. 1.Healthcare Research InstituteWakunaga Pharmaceutical Co. Ltd.HiroshimaJapan
  2. 2.School of Health SciencesSapporo Medical UniversitySapporoJapan

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