Plant Foods for Human Nutrition

, Volume 63, Issue 1, pp 41–46 | Cite as

Intracerebroventricular Administration of Soy Protein Hydrolysates Reduces Body Weight without Affecting Food Intake in Rats

  • Nerissa Vaughn
  • Anthony Rizzo
  • Dolores Doane
  • J. Lee Beverly
  • Elvira Gonzalez de Mejia
Original Paper


Some studies suggest that increased consumption of soy protein hydrolysates may cause body weight loss but the mechanism of action is unknown. The objective of this investigation was to determine whether intracerebroventricular (i.c.v.) infusion of soy protein hydrolysates decrease food intake and body weight. Adult male Sprague Dawley rats (n = 24) received i.c.v. injections of soy hydrolysate I (SH I) or soy hydrolysate II (SH II) three times weekly for 2 weeks. Krebs solution and leptin were used as negative and positive controls respectively. SH I (6.5–20 kDa with a strong band at 14 kDa) was produced by hydrolysis with alcalase, and SH II (∼2 kDa) was obtained by hydrolysis and ultrafiltration. Leptin successfully reduced body weight (−1.60 g) 24 h (p = 0.0093) after the third injection. SH I caused significant (p = 0.0009) decreases in body weight (−1.70 g) 24 h after the third injection but not after 48 h. SH II showed a tendency to prevent body weight gain but this effect was short of statistical significance (p < 0.40). Food intake was not affected by any of the soy hydrolysate treatments but leptin injection did cause significant decreases in food intake (p < 0.05). Data suggest that soy alcalase hydrolysate can decrease, in the short term, the rate of body weight gain independently of food consumption. This preliminary data show that soy peptides may play a role on body weight regulation, possibly by increasing energy utilization.


Leptin Obesity Soy protein Soy hydrolysate Body weight Food intake 



The authors wish to thank the Illinois Soybean Association, USDA Future Foods Initiative and SAI Company for their support of this research. Special thanks are also given to Wenyi Wang, and Maxine Roman for their help in conducting this research.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Nerissa Vaughn
    • 1
  • Anthony Rizzo
    • 1
  • Dolores Doane
    • 2
  • J. Lee Beverly
    • 2
  • Elvira Gonzalez de Mejia
    • 1
  1. 1.Department of Food Science & Human NutritionUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Department of Animal SciencesUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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