Plant Foods for Human Nutrition

, Volume 62, Issue 4, pp 145–150 | Cite as

Biochemical Characteristics of the Herb Mixture Prolipid as a Plant Food Supplement and Medicinal Remedy

  • Zenon Jastrzębski
  • Zev Tashma
  • Elena Katrich
  • Shela Gorinstein
Original Paper


Prolipid a known mixture of herbs is used as a plasma lipid lowering medicine. No side effects were registered. However, the bioactive substances of Prolipid were not investigated. Therefore in this investigation Prolipids bioactive compounds and antioxidant activity were studied. The contents of polyphenols and flavonoids were 19.87 ± 2.09 and 3.09 ± 0.31 mg gallic acid equivalent GAE/g DW and 2.09 ± 0.24 and 0.57 ± 0.05 mg catechin equivalent CE/g DW in water and methanol fractions, respectively. Anthocyanins (0.02 ± 0.001 mg/g DW) and flavanols (7.58 ± 0.81 μg CE/g DW) were found only in water fraction. The antioxidant activity of Prolipid, as determined by four different antioxidant assays [ferric-reducing/antioxidant power (FRAP); cupric reducing antioxidant capacity (CUPRAC); trolox equivalent antioxidant capacity (TEAC); 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH)], was higher in water than in methanol fraction. The correlation coefficients between polyphenols, flavonoids and antioxidant activities of Prolipid water extracts with TEAC were 0.97 and 0.90, respectively. It can be concluded that the content of polyphenol compounds in Prolipid is very high and they are the main contributors to Prolipids overall antioxidant activity. Prolipid is widely used in human treatment without known side effects on patients and is comparable to other medicinal plants, and as a strong antioxidant mixture could be used as a supplement to known atherosclerosis preventing diets.


Prolipid herb mixture Plant food supplement Antioxidants Antioxidant activity Radical scavenging assays 



Antioxidant activity


2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)


Butylated hydroxyanisole


Catechin equivalent


Cyanidin-3-glucoside equivalent


Cupric reducing antioxidant capacity


1,1-diphenyl-2-picrylhydrazyl radical


Epigallocatechin gallate equivalent


Ferric reducing antioxidant power


Gallic acid equivalent


Methanol fraction


Trolox equivalent antioxidant capacity




6-hydroxy-2,5,7,8,-tetramethyl-chroman-2-carboxylic acid


Water fraction



The authors are thankful to Prof. SIMON TRAKHTENBERG, Institute of Cardiology, Kaplan University Medical Center, Rehovot, Israel, for his assistance in medical and pharmacological interpretation of the results obtained.


  1. 1.
    Ivanova D, Gerova D, Chervenkov T, Yankova T (2005) Polyphenols and antioxidant capacity of Bulgarian medical plants. J Ethnopharmacol 96:145–150CrossRefGoogle Scholar
  2. 2.
    Katalinic V, Milos M, Kulisic T, Jukic M (2006) Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 94:550–557CrossRefGoogle Scholar
  3. 3.
    Katsube T, Tabata H, Ohta Y, Yamasaki Y, Anuurad E, Shiwaku K, Yamane Y (2004) Screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, DPPH, radical scavenging assay, and Folin-Ciocalteu assay. J Agric Food Chem 52:2391–2396CrossRefGoogle Scholar
  4. 4.
    Apak R, Gü, lü K, Özyürek M, Karademir SE, Er, ač E (2006) The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int J Food Sci Nutr 57:292–304CrossRefGoogle Scholar
  5. 5.
    Silva EM, Souza JNS, Rogez H, Rees JF, Larondelle Y (2007) Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region. Food Chem 101:1012–1018CrossRefGoogle Scholar
  6. 6.
    Ljubuncic P, Azaizeh H, Portnaya I, Cogan U, Said O, Saleh KA, Bomzon A (2005) Antioxidant activity and cytotoxicity of eight plants used in traditional Arab medicine in Israel. J Ethnopharmacol 99:43–47CrossRefGoogle Scholar
  7. 7.
    Szeto YT, Benzie IF (2006) Is the yin–yang nature of Chinese herbal medicine equivalent to antioxidation–oxidation? J Ethnopharmacol 108:361–366CrossRefGoogle Scholar
  8. 8.
    Ali N, Hashim NH, Saad B, Safan K, Nakajima M, Yoshizawa T (2005) Evaluation of a method to determine the natural occurrence of aflatoxins in commercial traditional herbal medicines from Malaysia and Indonesia. Food Chem Toxicol 43:1763–1772CrossRefGoogle Scholar
  9. 9.
    Fugh-Berman A (2000) Herbs and dietary supplements in the prevention and treatment of cardiovascular disease. Prev Cardiol 3:24–32CrossRefGoogle Scholar
  10. 10.
    Valli G, Giardina EGV (2002) Benefits, adverse effects and drug interactions of herbal therapies with cardiovascular effects. J Am Coll Cardiol 39:1083–1095CrossRefGoogle Scholar
  11. 11.
    Ozgen M, Reese RN, Tulio AZ, Scheerens JC, Miller AR (2006) Modified 2, 2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2, 2 -diphenyl-1-picrylhydrazyl (DPPH) methods. J Agric Food Chem 54:1151–1157CrossRefGoogle Scholar
  12. 12.
    Singh RP, Chidamdara M, Jayaprakasha GK (2002) Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agric Food Chem 50:81–86CrossRefGoogle Scholar
  13. 13.
    Ferrari R, Guardigli G, Mele D, Percoco GF, Ceconi C, Curello S (2004) Oxidative stress during myocardial ischaemia and heart failure. Curr Pharm Des 10:1699–1711CrossRefGoogle Scholar
  14. 14.
    Szeto YT, Tomlinson B, Benzie IFF (2002) Total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation. Br J Nutr 87:55–59CrossRefGoogle Scholar
  15. 15.
    Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, Brighenti F (2003) Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. J Nutr 133:2812–2819Google Scholar
  16. 16.
    Atta-ur-Rahman Shabbir M, Sultani Z, Jabbar A, Choudhary MI (1999) Cinnamates and coumarins from the leaves of Murraya paniculata. Phytochem 44:683–685CrossRefGoogle Scholar
  17. 17.
    Iyer UM, Mani UV (1990) Studies on the effect of curry leaves supplementation (Murraya koenigi) on lipid profile, glycated proteins and amino acids in non-insulin-dependent diabetic patients. Plant Foods Hum Nutr 40:275–282CrossRefGoogle Scholar
  18. 18.
    Yadav S, Vats V, Dhunoo Y, Grover JK (2002) Hypoglycemic and antihyperglycemic activity of Murraya koenigi leaves in diabetic rats. J Ethnopharmacol 82:111–116CrossRefGoogle Scholar
  19. 19.
    Fabricant DS, Farnsworth NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect Suppl 109:69–75CrossRefGoogle Scholar
  20. 20.
    Lotito SB, Frei B (2004) Relevance of apple polyphenols as antioxidants in human plasma: contrasting in vitro and in vivo effects. Free Radic Biol Med 36:201–211Google Scholar
  21. 21.
    Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M (2002) Free radical scavenging properties of wheat extracts. J Agric Food Chem 50:1619–1624CrossRefGoogle Scholar
  22. 22.
    Ou B, Huang D, Hampsch-Woodill M, Flanagan J, Deemer E (2002) Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study. J Agric Food Chem 50:3122–3128CrossRefGoogle Scholar
  23. 23.
    Friedman M, Juergens HS (2000) Effect of pH on the stability of plant phenolic compounds. J Agric Food Chem 48:2101–2110CrossRefGoogle Scholar
  24. 24.
    Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299:152–178CrossRefGoogle Scholar
  25. 25.
    Cheng GW, Breen PJ (1991) Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. J Am Soc Hortic Sci 116:865–869Google Scholar
  26. 26.
    Arnous A, Makris DP, Kefalas P (2001) Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. J Agric Food Chem 49:5736–5742CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Zenon Jastrzębski
    • 1
  • Zev Tashma
    • 2
  • Elena Katrich
    • 2
  • Shela Gorinstein
    • 2
  1. 1.Department of PharmacologyNational Medicines InstituteWarsawPoland
  2. 2.Department of Medicinal Chemistry and Natural Products, School of PharmacyThe Hebrew University-Hadassah Medical SchoolJerusalemIsrael

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