Antioxidative and Anticancer Activity of Extracts of Cherry (Prunus serrulata var. spontanea) Blossoms

  • Bo-Bae Lee
  • Mi-Ran Cha
  • Soo-Yeon Kim
  • Eunju Park
  • Hae-Ryong Park
  • Seung-Cheol Lee


Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 μg/ml) showed the highest total phenol content (104.30 μM), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE on DNA damage induced by H2O2 in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by 200 μM of H2O2, methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 μg/ml showed 38.8% inhibition against growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive materials.


Prunus serrulata var. spontanea Extract Antioxidant Anticancer 



cherry blossoms


cherry blossom extract


dimethyl sulfoxide




low melting agarose


3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide


phosphorous buffered saline


reactive oxygen species


reducing power


radical scavenging activity


total phenolic content



This study was supported by Kyungnam University Research Fund, 2007.


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Bo-Bae Lee
    • 1
  • Mi-Ran Cha
    • 1
  • Soo-Yeon Kim
    • 2
  • Eunju Park
    • 2
  • Hae-Ryong Park
    • 1
  • Seung-Cheol Lee
    • 1
  1. 1.Department of Food Science and BiotechnologyKyungnam UniversityMasanRepublic of Korea
  2. 2.Department of Food and NutritionKyungnam UniversityMasanRepublic of Korea

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