Q-band hyperchromism and B-band hypochromism of bacteriochlorophyll c as a tool for investigation of the oligomeric structure of chlorosomes of the green photosynthetic bacterium Chloroflexus aurantiacus

Abstract

Chlorosomes of green photosynthetic bacteria are the most amazing example of long-range ordered natural light-harvesting antennae. Chlorosomes are the largest among all known photosynthetic light-harvesting structures (~ 104–105 pigments in the aggregated state). The chlorosomal bacteriochlorophyll (BChl) c/d/e molecules are organized via self-assembly and do not require proteins to provide a scaffold for efficient light harvesting. Despite numerous investigations, a consensus regarding the spatial structure of chlorosomal antennae has not yet been reached. In the present work, we studied hyperchromism/hypochromism in the chlorosomal BChl c Q/B absorption bands of the green photosynthetic bacterium Chloroflexus (Cfx.) aurantiacus. The chlorosomes were isolated from cells grown under different light intensities and therefore, as we discovered earlier, they had different sizes of both BChl c antennae and their unit building blocks. We have shown experimentally that the Q-/B-band hyperchromism/hypochromism is proportional to the size of the chlorosomal antenna. We explained theoretically these findings in terms of excitonic intensity borrowing between the Q and B bands for the J-/H-aggregates of the BChls. The theory developed by Gülen (Photosynth Res 87:205–214, 2006) showed the dependence of the Q-/B-band hyperchromism/hypochromism on the structure of the aggregates. For the model of exciton-coupled BChl c linear chains within a unit building block, the theory predicted an increase in the hyperchromism/hypochromism with the increase in the number of molecules per chain and a decrease in it with the increase in the number of chains. It was previously shown that this model ensured a good fit with spectroscopy experiments and approximated the BChl c low packing density in vivo. The presented experimental and theoretical studies of the Q-/B-band hyperchromism/hypochromism permitted us to conclude that the unit building block of Cfx. aurantiacus chlorosomes comprises of several short BChl c chains.

This conclusion is in accordance with previous linear and nonlinear spectroscopy studies on Cfx. aurantiacus chlorosomes.

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Abbreviations

BChl:

Bacteriochlorophyll

Cfx :

Chloroflexus

CMC:

Chlorosome-membrane complexes

Cba :

Chlorobaculum

OD:

Optical density

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Acknowledgements

The authors are very grateful to Prof. Dr. Demet Gülen for fruitful cooperation and assistance in theoretical modeling. This work was supported in part by the Russian Foundation for Basic Research (Grants 18-04-00105a, 14-04-00295a).

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Yakovlev, A.G., Taisova, A.S. & Fetisova, Z.G. Q-band hyperchromism and B-band hypochromism of bacteriochlorophyll c as a tool for investigation of the oligomeric structure of chlorosomes of the green photosynthetic bacterium Chloroflexus aurantiacus. Photosynth Res 146, 95–108 (2020). https://doi.org/10.1007/s11120-019-00707-9

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Keywords

  • Bacterial photosynthesis
  • Green bacteria
  • Chloroflexus aurantiacus
  • Chlorosome
  • Bacteriochlorophyll c antenna