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Redox regulation by peroxiredoxins is linked to their thioredoxin-dependent oxidase function

Abstract

The chloroplast contains three types of peroxiredoxins (PRXs). Recently, 2-CysPRX was associated with thioredoxin (TRX) oxidation-dependent redox regulation. Here, this analysis was expanded to include PRXQ and PRXIIE. Oxidized PRXQ was able to inactivate NADPH malate dehydrogenase and fructose-1,6-bisphosphatase most efficiently in the presence of TRX-m1 and TRX-m4. The inactivation ability of TRXs did not entirely match their reductive activation efficiency. PRXIIE was unable to function as TRX oxidase in enzyme regulation. This conclusion was further supported by the observation that PRXQ adopts the oxidized form by about 50% in leaves, supporting a possible function as a TRX oxidase similar to 2-CysPRX. Results on the oxidation state of photosystem I (P700), plastocyanin, and ferredoxin in intact leaves indicate that each type of PRX has distinct regulatory functions, and that both 2-CysPRX and PRXQ conditionally assist in adjusting the redox state of target proteins for proper activity.

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Acknowledgements

This work was supported by the DFG (DI346/17, SPP1710). We are grateful to Anna Dreyer for providing the PRXIIE expression system, Corinna Wesemann for the A. thaliana pad2 plant material, and Martina Holt for technical assistance.

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WT performed biochemical and enzymatic assays, ML growth experiments in fluctuating light, and analyses of photosynthetic parameters. K-JD designed and directed the project. All authors wrote, read, and approved the final manuscript.

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Correspondence to Karl-Josef Dietz.

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Telman, W., Liebthal, M. & Dietz, KJ. Redox regulation by peroxiredoxins is linked to their thioredoxin-dependent oxidase function. Photosynth Res 145, 31–41 (2020). https://doi.org/10.1007/s11120-019-00691-0

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Keywords

  • A. thaliana
  • Calvin–Benson cycle
  • Malate valve
  • Peroxiredoxins
  • Redox regulation
  • Thioredoxins