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Complementary chromatic and far-red photoacclimations in Synechococcus ATCC 29403 (PCC 7335). I: The phycobilisomes, a proteomic approach

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Abstract

Synechococcus ATCC 29403 (PCC 7335) is a unicellular cyanobacterium isolated from Puerto Peñasco, Sonora Mexico. This cyanobacterium performs complementary chromatic acclimation (CCA), far-red light photoacclimation (FaRLiP), and nitrogen fixation. The Synechococcus PCC 7335 genome contains at least 31 genes for proteins of the phycobilisome (PBS). Nine constitutive genes were expressed when cells were grown under white or red lights and the resulting proteins were identified by mass spectrometry in isolated PBS. Five inducible genes were expressed under white light, and phycoerythrin subunits and associated linker proteins were detected. The proteins of five inducible genes expressed under red light were identified, the induced phycocyanin subunits, two rod linkers and the rod-capping linker. The five genes for FaRLiP phycobilisomes were expressed under far-red light together with the apcF gene, and the proteins were identified by mass spectrometry after isoelectric focusing and SDS-PAGE. Based on in silico analysis, Phylogenetic trees, and the observation of a highly conserved amino acid sequence in far-red light absorbing alpha allophycoproteins encoded by FaRLiP gene cluster, we propose a new nomenclature for the genes. Based on a ratio of ApcG2/ApcG3 of six, a model with the arrangement of the allophycocyanin trimers of the core is proposed.

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Abbreviations

AP:

Allophycocyanin

App Mw:

Apparent molecular weight

CCA:

Complementary chromatic acclimation

Core-PBS:

Bicylindrical core containing far-red light absorbing allophycoproteins encoded by FaRLiP gene cluster (Ho et al. 2017a, b)

DTT:

Dithiothreitol

FRL:

Far-red light

FaRLiP:

Far-red light photoacclimation

GL:

Green light

Hhc:

Horse heart cytochrome c

IEF:

Isoelectric focusing

LC :

Linker core

LCM :

Linker core membrane

LR :

Linker rod

LRC :

Linker rod core

PBS:

Phycobilisome

PBP:

Phycobiliprotein

PC:

Phycocyanin

PC-PBS:

Phycocyanin phycobilisome

PCB:

Phycocyanobilin

PE:

Phycoerythrin

PE-PBS:

Phycoerythrin phycobilisome

PS:

Photosystem

PMSF:

Phenylmethylsulfonyl fluoride

REP:

Repetitive linker-like sequence

RL:

Red light

WCE:

Whole-cell extracts

WL:

White light

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Acknowledgements

This paper is dedicated to the memory of Dr. David W. Krogmann, professor, colleague, and friend. PHS received scholarship from Consejo Nacional de Ciencia y Tecnología (CONACYT, scholarship no. 261987. Dra. Emma Berta Gutiérrez-Cirlos and Dr. Diego González Halphen for his suggestions. Dr. Marco Antonio Meraz Ríos for his discussions and support in acquisition of reagents. We also thank the reviewers for the improvement of the manuscript. The authors wish to thank Mr Jorge Zarco Mendoza for his assistance to improve the technical details in the centrifugation, French press to break cells and to start the cultures. Dra. Karla Grisel Calderón-González for her technical assistance in the IEF. We also thank Mrs Leticia Gómez Sandoval for her secretarial assistance.

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  1. Departamento de Bioquímica, Centro de Investigación y Estudios Avanzados del IPN, Mexico City, Mexico

    Priscila Herrera-Salgado, Lourdes E. Leyva-Castillo, Emmanuel Ríos-Castro & Carlos Gómez-Lojero

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  1. Priscila Herrera-Salgado
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  2. Lourdes E. Leyva-Castillo
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  3. Emmanuel Ríos-Castro
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Correspondence to Carlos Gómez-Lojero.

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Herrera-Salgado, P., Leyva-Castillo, L.E., Ríos-Castro, E. et al. Complementary chromatic and far-red photoacclimations in Synechococcus ATCC 29403 (PCC 7335). I: The phycobilisomes, a proteomic approach. Photosynth Res 138, 39–56 (2018). https://doi.org/10.1007/s11120-018-0536-6

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