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Regulation of excitation energy in Nannochloropsis photosystem II

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Abstract

Recently, we isolated a complex consisting of photosystem II (PSII) and light-harvesting complexes (LHCs) from Nannochloropsis granulata (Umetani et al. Photosynth Res 136:49–61, 2017). This complex contained stress-related protein, Lhcx, as a major component of LHC (Protein ID is Naga_100173g12.1), suggesting that non-photochemical quenching activities may be taking place in the PSII-LHC complex. In this study, we examined the energy transfer dynamics in the isolated LHCs and PSII-LHC complexes, and found substantial quenching capacity. In addition, the LHCs contained low-energy chlorophylls with fluorescence maxima at approximately 710 nm, which may enhance the quenching efficiency in the PSII-LHC. Delayed fluorescence analysis suggested that there was an approximately 50% reduction in energy trapping at the PSII reaction center in the PSII-LHC supercomplex under low-pH condition compared to neutral pH condition. Enhanced quenching may confer a survival advantage in the shallow-water habitat of Nannochloropsis.

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Abbreviations

PSII:

Photosystem II

LHC:

Light-harvesting complex

CN-PAGE:

Clear-native polyacrylamide gel electrophoresis

FDAS:

Fluorescence decay-associated spectra

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Acknowledgements

This work was supported by the Japan Society for the Promotion of Science (KAKENHI Grant No. 16H06553 to S. Akimoto, grant-in-aid for Young Scientists 23770035 to A. Takabayashi, and Scientific Research Grant 24370017 to A. Tanaka) and the University College of Southeast Norway. We thank Shelley Robison, PhD, from Edanz Group (http://www.edanzediting.com/ac) for editing a draft of this manuscript.

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Authors and Affiliations

  1. Innovation Center, Nippon Flour Mills Co., Ltd., Atsugi, 243-0041, Japan

    Makio Yokono

  2. Institute of Low Temperature Science, Hokkaido University, N19 W8 Kita-Ku, Sapporo, 060-0819, Japan

    Makio Yokono, Ikumi Umetani, Atushi Takabayashi & Ayumi Tanaka

  3. Department of Natural Sciences and Environmental Health, University College of Southeast Norway, Gullbringvegen 36, 3880, Bø, Norway

    Ikumi Umetani

  4. Graduate School of Science, Kobe University, Kobe, 657-8501, Japan

    Seiji Akimoto

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  1. Makio Yokono
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  2. Ikumi Umetani
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  3. Atushi Takabayashi
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  4. Seiji Akimoto
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  5. Ayumi Tanaka
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Correspondence to Makio Yokono.

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M.Y. is employee of NIPPON FLOUR MILLS Co.,Ltd.

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Yokono, M., Umetani, I., Takabayashi, A. et al. Regulation of excitation energy in Nannochloropsis photosystem II. Photosynth Res 139, 155–161 (2019). https://doi.org/10.1007/s11120-018-0510-3

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  • DOI: https://doi.org/10.1007/s11120-018-0510-3

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