Far-red light photoacclimation (FaRLiP) in Synechococcus sp. PCC 7335. II.Characterization of phycobiliproteins produced during acclimation to far-red light
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Phycobilisomes (PBS) are antenna complexes that harvest light for photosystem (PS) I and PS II in cyanobacteria and some algae. A process known as far-red light photoacclimation (FaRLiP) occurs when some cyanobacteria are grown in far-red light (FRL). They synthesize chlorophylls d and f and remodel PS I, PS II, and PBS using subunits paralogous to those produced in white light. The FaRLiP strain, Leptolyngbya sp. JSC-1, replaces hemidiscoidal PBS with pentacylindrical cores, which are produced when cells are grown in red or white light, with PBS with bicylindrical cores when cells are grown in FRL. This study shows that the PBS of another FaRLiP strain, Synechococcus sp. PCC 7335, are not remodeled in cells grown in FRL. Instead, cells grown in FRL produce bicylindrical cores that uniquely contain the paralogous allophycocyanin subunits encoded in the FaRLiP cluster, and these bicylindrical cores coexist with red-light-type PBS with tricylindrical cores. The bicylindrical cores have absorption maxima at 650 and 711 nm and a low-temperature fluorescence emission maximum at 730 nm. They contain ApcE2:ApcF:ApcD3:ApcD2:ApcD5:ApcB2 in the approximate ratio 2:2:4:6:12:22, and a structural model is proposed. Time course experiments showed that bicylindrical cores were detectable about 48 h after cells were transferred from RL to FRL and that synthesis of red-light-type PBS continued throughout a 21-day growth period. When considered in comparison with results for other FaRLiP cyanobacteria, the results here show that acclimation responses to FRL can differ considerably among FaRLiP cyanobacteria.
KeywordsCyanobacteria Photoacclimation Chlorophyll f Far-red light Photosynthesis
This research was supported by Grant MCB-1021725 from the National Science Foundation to D. A. B. This research was also conducted under the auspices of the Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center funded by the DOE, Office of Science, Office of Basic Energy Sciences, under Award Number DE-SC 0001035.
- Akutsu S, Fujinuma D, Furukawa H, Watanabe T, Ohnishi-Kameyama M, Ono H, Ohkubo S, Miyashita H, Kobayashi M (2011) Pigment analysis of a chlorophyll f-containing cyanobacterium isolated from Lake Biwa. Photomed Photobiol 33:36–40Google Scholar
- Brown II, Bryant DA, Casamatta D, Thomas-Keprta KL, Sarkisova SA, Shen G, Graham JE, Boyd ES, Peters JW, Garrison DH, McKay DS (2010) Polyphasic characterization of a thermotolerant siderophilic filamentous cyanobacterium that produces intracellular iron deposits. Appl Environ Microbiol 76:6664–6672CrossRefPubMedPubMedCentralGoogle Scholar
- Bryant DA (1982) Phycoerythrocyanin and phycoerythrin: properties and occurrence in cyanobacteria. J Gen Microbiol 128:835–844Google Scholar
- Bryant DA (1988) Genetic analysis of phycobilisome biosynthesis, assembly, structure, and function in the cyanobacterium Synechococcus sp. PCC 7002. In: Stevens SE Jr, Bryant DA (eds) Light-energy transduction in photosynthesis: higher plant and bacterial models. American Society of Plant Biologists, Rockville, pp 62–90Google Scholar
- Bryant DA (1991) Cyanobacterial phycobilisomes: progress toward complete structural and functional analysis via molecular genetics. In: Bogorad L, Vasil I (eds) Cell culture and somatic cell genetics of plants, volume 7B, the photosynthetic apparatus: molecular biology and operation. Academic Press, New York, pp 257–300CrossRefGoogle Scholar
- Glazer AN, Lundell DJ, Yamanka G, Williams RC (1983) The structure of a “simple” phycobilisome. Ann Microbiol 134B:159–180Google Scholar
- Miao D, Ding W-L, Zhao B-Q, Lu L, Xu Q-Z, Scheer H, Zhao K-H (2016) Adapting photosynthesis to the near-infrared: non-covalent binding of phycocyanobilin provides an extreme spectral red-shift to phycobilisome core-membrane linker from Synechococcus sp. PCC7335. Biochim Biophys Acta 1857:688–694CrossRefPubMedGoogle Scholar
- Sidler WA (1994) Phycobilisome and phycobiliprotein structure. In: Bryant DA (ed) Advances in photosynthesis and respiration, volume 1, the molecular biology of cyanobacteria. Kluwer Academic, Dordrecht, pp 139–216Google Scholar
- Zhao J, Zhou J, Bryant DA (1992) Energy transfer processes in phycobilisomes as deduced from analyses of mutants of Synechococcus sp. PCC 7002. In: Murata N (ed) Research in photosynthesis, vol 1. Kluwer Academic Publishers, Dordrecht, pp 25–32Google Scholar