Photosynthesis Research

, Volume 124, Issue 2, pp 217–229 | Cite as

Characterization of a Synechocystis sp. PCC 6803 double mutant lacking the CyanoP and Ycf48 proteins of Photosystem II

  • Simon A. Jackson
  • Julian J. Eaton-Rye
Regular Paper


Homologs of the Photosystem II (PS II) subunit PsbP are found in plants, algae, and cyanobacteria. In higher plants, PsbP is associated with mature PS II centers, but in cyanobacteria, the homologous CyanoP protein appears sub-stoichiometric to PS II. We have investigated the role of CyanoP by characterizing knockout mutants of the cyanobacterium Synechocystis sp. PCC 6803. Removal of CyanoP resulted in changes to phycobilisome coupling and energy transfer to PS II, but the function of PS II itself remained similar to wild type. We therefore investigated the hypothesis that CyanoP is involved in the biogenesis or repair of PS II by creating a double mutant lacking both CyanoP and the PS II assembly factor Ycf48. This strain exhibited an additive reduction in the amplitude of variable chlorophyll a fluorescence induction relative to either of the single mutants but displayed increased oxygen evolution, slight increases in PS II monomer and dimer levels, and a reduction in accumulation of an early PS II assembly complex containing CP47, compared to the ΔYcf48 strain.


Assembly Biogenesis CyanoP Photosystem II PsbP Synechocystis sp. PCC 6803 Ycf48 





Blue measuring flashes


Blue-native polyacrylamide gel electrophoresis


43-kDa chlorophyll a-binding protein of the core antenna


47-kDa chlorophyll a-binding protein of the core antenna


Photosystem II reaction center protein subunit


Photosystem II reaction center protein subunit




Dark-adapted (minimum) fluorescence


Maximum level of chlorophyll a fluorescence

J and I

Inflection points between O (FO) and P in the chlorophyll a fluorescence induction curve


Liquid chromatography tandem mass spectrometry


Light-harvesting complex


Origin (FO) level of the chlorophyll a fluorescence induction curve


Optical density


Peak level in the chlorophyll a fluorescence induction curve


Photosystem I


Photosystem II


Primary plastoquinone electron acceptor of PS II


Secondary plastoquinone electron acceptor of PS II


Reaction center complex lacking the CP43 protein


Red measuring flashes


Oxidation states of the oxygen-evolving complex of PS II


Sodium dodecyl sulfate polyacrylamide gel electrophoresis





S.A.J. was supported by a University of Otago Division of Health Sciences Career Development Postdoctoral Fellowship. Other funding for this project was provided by an Otago University Research grant to J.E.-R. We thank Asher J. Dale for assistance with preliminary experiments.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of OtagoDunedinNew Zealand

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