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Seasonal changes in the content of dehydrins in mesophyll cells of common pine needles

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Abstract

The appearance of dehydrins (DHNs) in cells is required for the development of cold resistance. DHNs are therefore considered specific markers of cold resistance by some authors. DHNs accumulate in plants concomitantly with a reduction of intracellular water content, and presumably protect membranes and proteins from damage caused by moisture loss. DHN content in pine needles increases in spring and autumn when moisture availability and temperatures are most unfavorable. The present work is focused on seasonal changes in DHN content in various mesophyll-cell compartments of pine (Pinus sylvestris L.) needles in association with changes in environmental factors. In spring, the number of thylakoid membranes per granum was lower than in summer and autumn. An increase in needle content of DHNs with approximate masses of 76, 73, 72, 35, and 17 kD in spring and autumn, associated with needle dehydration during this period, is shown here. The largest increase in DHN content was observed in spring, with the highest amount of DHNs presented in chloroplast membrane system including grana thylakoids, stromal thylakoids, and the two chloroplast envelope membranes and in cell walls. In the autumn, most DHNs were localized in chloroplasts and mitochondria.

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Acknowledgments

The authors express their gratitude to L.D. Kopytova and L.S. Yan’kova, PhD, for their contribution to the acquisition of experimental data.

Funding

Program of Russian Academy of Sciences “Wildlife: present state and development problems” (2013); Baikal Analytic Core Facility of the Irkutsk Scientific Centre.

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  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of Russian Academy of Sciences, Lermontova St., 132, POB 317, 664033, Irkutsk, Russia

    Natalia Korotaeva, Anatolii Romanenko, Galina Suvorova, Maria V. Ivanova, Lidia Lomovatskaya, Gennadii Borovskii & Victor Voinikov

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  1. Natalia Korotaeva
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  2. Anatolii Romanenko
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  3. Galina Suvorova
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  4. Maria V. Ivanova
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Correspondence to Maria V. Ivanova.

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Supplementary Fig. 1.

Change of ASWS in 2007. For data analysis, values of total reserves in the 0–50 cm layer of soil were used. Soil moisture contents were determined for each 10 cm soil layer to a 50 cm depth every ten days during the growth period using the thermostat-gravimetric method. The available soil water supply (ASWS) was calculated by a commonly used technique (Fedorovsky 1975) as the difference between the soil moisture content and the moisture inaccessible for plants, which taken to be equal to the maximum amount of hygroscopic water held by soil particles multiplied by a factor of 1.5. Supplementary material 2 (TIFF 13,894 kb)

Supplementary Fig. 2

Change of the free water content (FWC) in pine needles in 2007. The content of free water in the needles was determined by the refractometric method as a percentage of the total amount of water released into a 30 % solution of sucrose. Each replication contained 0.6 g of needles containing equal quota of the needles from each of three pines. Standard deviations are presented. n = 6; p ≤ 0.05. Supplementary material 3 (TIFF 13,196 kb)

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Korotaeva, N., Romanenko, A., Suvorova, G. et al. Seasonal changes in the content of dehydrins in mesophyll cells of common pine needles. Photosynth Res 124, 159–169 (2015). https://doi.org/10.1007/s11120-015-0112-2

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