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Orientation of B798 BChl a Q y transition dipoles in Chloroflexus aurantiacus chlorosomes: polarized transient absorption spectroscopy studies

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Abstract

Isotropic and anisotropic pump-probe spectra of Cfx. aurantiacus chlorosomes were measured on the fs-through ps-time scales for the B798 BChl a Q y band upon direct excitation of the B798 band at T = 293 K and T = 90 K. Upon direct excitation of the B798 band, the anisotropy parameter value r(λ) was constant within the whole BChl a Q y band at any delay time at both temperatures. The value of the anisotropy parameter r decayed from r = 0.4 at both temperatures (at 200 fs delay time after excitation) to the steady-state values r = 0.1 at T = 293 K and to r = 0.09 at T = 90 K (at 30 ÷ 100 ps delay time after excitation). The results were considered within the framework of the model of uniaxial orientation distribution of BChl-a transition dipoles within a single Cfx. aurantiacus chlorosome. This implies that the B798 BChl a Q y transition dipoles, randomly distributed around the normal to the baseplate plane, form the angle θ with the plane. For this model, the theoretical dependence of the steady-state anisotropy parameter r on the angle θ was derived. According to the theoretical dependence r(θ), the angle θ corresponding to the experimental steady-state value r = 0.1 at T = 293 K was found to equal 55°. As the temperature drops to 90 K, the angle θ decreases to 54°.

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Acknowledgments

The work was supported by the Russian Foundation for Basic Research (Grant 08-04-01587a, 10-04-01758-a).

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  1. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, 142290, Moscow, Russian Federation

    Vladimir Shuvalov

  2. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie Gory, 119991, Moscow, Russian Federation

    Andrei Yakovlev, Vladimir Novoderezhkin, Alexandra Taisova, Vladimir Shuvalov & Zoya Fetisova

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  1. Andrei Yakovlev
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Correspondence to Zoya Fetisova.

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Yakovlev, A., Novoderezhkin, V., Taisova, A. et al. Orientation of B798 BChl a Q y transition dipoles in Chloroflexus aurantiacus chlorosomes: polarized transient absorption spectroscopy studies. Photosynth Res 125, 31–42 (2015). https://doi.org/10.1007/s11120-014-0060-2

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