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Photosynthesis Research

, Volume 125, Issue 1–2, pp 141–150 | Cite as

Brassica napus responses to short-term excessive copper treatment with decrease of photosynthetic pigments, differential expression of heavy metal homeostasis genes including activation of gene NRAMP4 involved in photosystem II stabilization

  • I. E. Zlobin
  • V. P. Kholodova
  • Z. F. Rakhmankulova
  • Vl. V. Kuznetsov
Regular Paper

Abstract

In the present study, the influence of 50 and 100 µM CuSO4 was investigated starting from 3 h till 72 h treatment of 4-weeks Brassica napus plants. High CuSO4 concentrations in nutrient medium resulted in the rapid copper accumulation in plants, especially in roots, much slower and to lower degree in leaves. Copper excess induced early decrease in the leaf water content and temporary leaf wilting. The decrease in content of photosynthetic pigments became significant to 24 h of excessive copper treatments and reached 35 % decrease to 72 h, but there were no significant changes in maximum quantum efficiency of photosystem II photochemistry. The copper excess affected the expression of ten genes involved in heavy metal homeostasis and copper detoxification. The results showed the differential and organ-specific expression of most genes. The potential roles of copper-activated genes encoding heavy metal transporters (ZIP5, NRAMP4, YSL2, and MRP1), metallothioneins (MT1a and MT2b), low-molecular chelator synthesis enzymes (PCS1 and NAS2), and metallochaperones (CCS and HIPP06) in heavy metal homeostasis and copper ion detoxification were discussed. The highest increase in gene expression was shown for NRAMP4 in leaves in spite of relatively moderate Cu accumulation there. The opinion was advanced that the NRAMP4 activation can be considered among the early reactions in the defense of the photosystem II against copper excess.

Keywords

Brassica napus Copper detoxification Copper excess Photosynthetic pigments Photosystem II photochemistry Gene expression 

Abbreviations

ATX1

Antioxidant 1-like

CCS

Copper chaperone for Cu/Zn superoxide dismutase

Car

Carotin

Chl

Chlorophyll

COPT

Copper transporter

DW

Dry weight

F0

Minimal fluorescence yield of dark-adapted state

Fm

Maximal fluorescence yield of dark-adapted state

Fv

Variable fluorescence = F m − F 0

Fv/Fm

Maximal quantum yield of PSII photochemistry

FW

Fresh weight

HIPP

Heavy metal-associated isoprenylated plant protein

HM

Heavy metal

MRP

Multidrug resistance-associated protein homolog

MT

Metallothionein

NAS

Nicotianamine synthase

NRAMP

Natural resistance-associated macrophage protein

PC

Phytochelatin

PCS

Phytochelatin synthase

ROS

Reactive oxygen species

PS

Photosystem

Xan

Xanthophyll

YSL

Yellow stripe-like

ZIP

Zrt-, Irt-like protein

Notes

Acknowledgments

This study was supported through funding from the Russian Foundation for Basic Research, project no. 13-04-01001, and the Presidium of the Russian Academy of Sciences (Molecular and Cellular Biology Program).

Supplementary material

11120_2014_54_MOESM1_ESM.doc (1.1 mb)
Supplementary material 1 (DOC 1094 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • I. E. Zlobin
    • 1
  • V. P. Kholodova
    • 1
  • Z. F. Rakhmankulova
    • 1
  • Vl. V. Kuznetsov
    • 1
    • 2
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.Department of Plant PhysiologyMoscow State UniversityMoscowRussia

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