Photosynthesis Research

, Volume 117, Issue 1–3, pp 257–265 | Cite as

Low-temperature time-resolved spectroscopic study of the major light-harvesting complex of Amphidinium carterae

  • Václav Šlouf
  • Marcel Fuciman
  • Silke Johanning
  • Eckhard Hofmann
  • Harry A. Frank
  • Tomáš Polívka
Regular Paper


The major light-harvesting complex of Amphidinium (A.) carterae, chlorophyll-a–chlorophyll-c 2–peridinin–protein complex (acpPC), was studied using ultrafast pump-probe spectroscopy at low temperature (60 K). An efficient peridinin–chlorophyll-a energy transfer was observed. The stimulated emission signal monitored in the near-infrared spectral region was stronger when redder part of peridinin pool was excited, indicating that these peridinins have the S1/ICT (intramolecular charge-transfer) state with significant charge-transfer character. This may lead to enhanced energy transfer efficiency from “red” peridinins to chlorophyll-a. Contrary to the water-soluble antenna of A. carterae, peridinin–chlorophyll-a protein, the energy transfer rates in acpPC were slower under low-temperature conditions. This fact underscores the influence of the protein environment on the excited-state dynamics of pigments and/or the specificity of organization of the two pigment–protein complexes.


Light-harvesting Energy transfer Carotenoid Dinoflagellates 



The authors thank Miriam Enriquez for help with measuring the absorption spectra at 77 K. The work in Czech Republic was supported by the Czech Science Foundation (P501/12/G055 and P205/11/1164), and project KONTAKT ME09037 from the Ministry of Education of the Czech Republic. Work in the laboratory of HAF was supported by grants from the National Science Foundation (MCB-1243565) and the University of Connecticut Research Foundation.

Supplementary material

11120_2013_9900_MOESM1_ESM.docx (123 kb)
Supplementary material 1 (DOCX 122 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Václav Šlouf
    • 1
  • Marcel Fuciman
    • 1
    • 3
  • Silke Johanning
    • 2
  • Eckhard Hofmann
    • 2
  • Harry A. Frank
    • 3
  • Tomáš Polívka
    • 1
    • 4
  1. 1.Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  2. 2.AG Proteincrystallography, Department of Biophysics, Faculty of Biology and BiotechnologyRuhr University BochumBochumGermany
  3. 3.Department of ChemistryUniversity of ConnecticutStorrsUSA
  4. 4.Biological CentreCzech Academy of SciencesČeské BudějoviceCzech Republic

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