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Modification of a French pressure cell to improve microbial cell disruption

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Abstract

A procedure for modification of the valve stem of a 40 K French pressure cell is described. The modification should be done by a machinist and requires a metalworking lathe. After modification of the valve stem, a torlon 4203 plastic ball is used between the valve stem and valve seat to control the pressure within the cell. The torlon plastic ball is a key component needed to obtain the high pressures required for efficient disruption of microbial cells.

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References

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada

    Paul R. Jaschke & J. Thomas Beatty

  2. The Life Sciences Centre—Equipment Services Workshop, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, V6T 1Z3, Canada

    Ian Drake

Authors
  1. Paul R. Jaschke
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  2. Ian Drake
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  3. J. Thomas Beatty
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Corresponding author

Correspondence to J. Thomas Beatty.

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Jaschke, P.R., Drake, I. & Beatty, J.T. Modification of a French pressure cell to improve microbial cell disruption. Photosynth Res 102, 95–97 (2009). https://doi.org/10.1007/s11120-009-9493-4

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  • DOI: https://doi.org/10.1007/s11120-009-9493-4

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