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Photosynthesis Research

, Volume 100, Issue 1, pp 19–28 | Cite as

Singlet oxygen formation and chlorophyll a triplet excited state deactivation in the cytochrome b 6 f complex from Bryopsis corticulans

  • Fei Ma
  • Xiao-Bo Chen
  • Min Sang
  • Peng Wang
  • Jian-Ping Zhang
  • Liang-Bi Li
  • Ting-Yun Kuang
Regular Paper

Abstract

We have attempted to investigate the correlation between the detergent-perturbed structural integrity of the Cyt b 6 f complex from the marine green alga Bryopsis corticulans and its photo-protective properties, for which the nonionic detergents n-octyl-β-d-glucopyranoside (β-OG) and n-dodecyl-β-d-maltoside (β-DM), respectively, were used for the preparation of Cyt b 6 f, and the singlet oxygen (1O2*) production as well as the triplet excited-state chlorophyll a (3Chl a*) formation and deactivation were examined by spectroscopic means. Near-infrared luminescence of 1O2 * (~1,270 nm) on photo-irradiation was detected for the β-OG preparation where the complex is mainly in oligomeric state, but not for the β-DM one in which the complex exists in dimeric form. Under anaerobic condition, photo-excitation of Chl a in the β-DM preparation generated 3Chl a* with a lower quantum yield of ΦT ~ 0.02 and a longer lifetime of ~600 μs with respect to those as in the case of β-OG preparation, ΦT ~ 0.12 and 200–300 μs. These results prove that the enzymatically active and intact Cyt b 6 f complex on photo-excitation tends to produce little 3Chl a* or 1O2 *, which implies that the pigment–protein assembly of Cyt b 6 f complex per se is crucial for photo-protection.

Keywords

Cytochrome b6f Photo protection Singlet oxygen Triplet excited state Excitation energy transfer 

Abbreviations

Car

Carotenoid

3Chl a*

Triplet excited state chlorophyll a

Cyt b6f

Cytochrome b 6 f complex

β-DM

n-Dodecyl-β-d-maltoside

EET

Excitation energy transfer

1O2*

Singlet oxygen

β-OG

n-Octyl-β-d-glucopyranoside

SDS

Sodium dodecyl sulfate

ΦT

Quantum yield of triplet excited state chlorophyll a

Notes

Acknowledgments

We are grateful for the assistance in DLS experiments by Dr. Yi-Wei Liu at the Department of Biological Science and Biotechnology, Tsinghua University. This work has been jointly supported by the National Basic Research Program of China (2009CB220008), the Natural Science Foundation of China (20673144 and 20703067) and the Natural Science Foundation of Hebei Province (C2008000684).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Beijing National Laboratory for Molecular Science (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of ChemistryChinese Academy of SciencesBeijingChina
  2. 2.Department of ChemistryRenmin University of ChinaBeijingChina
  3. 3.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of BotanyChinese Academy of SciencesBeijingChina

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