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Photosynthesis Research

, Volume 99, Issue 1, pp 49–61 | Cite as

Photosystem II efficiency of the palisade and spongy mesophyll in Quercus coccifera using adaxial/abaxial illumination and excitation light sources with wavelengths varying in penetration into the leaf tissue

  • José Javier Peguero-Pina
  • Eustaquio Gil-Pelegrín
  • Fermín Morales
Regular Paper

Abstract

The existence of major vertical gradients within the leaf is often overlooked in studies of photosynthesis. These gradients, which involve light heterogeneity, cell composition, and CO2 concentration across the mesophyll, can generate differences in the maximum potential PSII efficiency (F V/F M or F V/F P) of the different cell layers. Evidence is presented for a step gradient of F V/F P ratios across the mesophyll, from the adaxial (palisade parenchyma, optimal efficiencies) to the abaxial (spongy parenchyma, sub-optimal efficiencies) side of Quercus coccifera leaves. For this purpose, light sources with different wavelengths that penetrate more or less deep within the leaf were employed, and measurements from the adaxial and abaxial sides were performed. To our knowledge, this is the first report where a low photosynthetic performance in the abaxial side of leaves is accompanied by impaired F V/F P ratios. This low photosynthetic efficiency of the abaxial side could be related to the occurrence of bundle sheath extensions, which facilitates the penetration of high light intensities deep within the mesophyll. Also, leaf morphology (twisted in shape) and orientation (with a marked angle from the horizontal plane) imply direct sunlight illumination of the abaxial side. The existence of cell layers within leaves with different photosynthetic efficiencies makes appropriate the evaluation of how light penetrates within the mesophyll when using Chl fluorescence or gas exchange techniques that use different wavelengths for excitation and/or for driving photosynthesis.

Keywords

Abaxial Adaxial Cell layers Chlorophyll fluorescence Photosynthesis Step gradient Mesophyll 

Abbreviations

A

Net CO2 uptake

BSE

Bundle sheath extensions

Chl

Chlorophyll

ETR

Electron transport rate

ΦPSII and Φexc.

Actual and intrinsic photosystem II efficiencies, respectively

FO and \(F^{\prime}_{O}\)

Minimal Chl fluorescence yield in the dark or during light adaptation, respectively

FM and \(F^{\prime}_{M}\)

Maximal Chl fluorescence yield in the dark or during light adaptation, respectively

FS

Chl fluorescence at steady-state photosynthesis

FP

Chl fluorescence at the peak of the continuous fluorescence induction curve

FV and \(F^{\prime}_{V}\)

FM − FO or FP − FO, and \(F^{\prime}_{M}\) − \(F^{\prime}_{O}\), respectively

gS

Stomatal conductance

NPQ

Non-photochemical quenching

PPFD

Photosynthetic photon flux density

PSI and PSII

Photosystems I and II, respectively

qP

Photochemical quenching

Notes

Acknowledgments

This work was supported by INIA project RTA01-071-C3-1 (Ministerio de Educación y Ciencia), and Gobierno de Aragón. Authors acknowledge comments of two anonymous reviewers that largely improved this manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • José Javier Peguero-Pina
    • 1
  • Eustaquio Gil-Pelegrín
    • 1
  • Fermín Morales
    • 2
  1. 1.Unidad de Recursos ForestalesCentro de Investigación y Tecnología Agroalimentaria, Gobierno de AragónZaragozaSpain
  2. 2.Department of Plant NutritionExperimental Station of Aula Dei, CSICZaragozaSpain

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