Photosynthesis Research

, Volume 94, Issue 2–3, pp 315–320 | Cite as

Estimation of relative contribution of “mobile phycobilisome” and “energy spillover” in the light–dark induced state transition in Spirulina platensis

  • Rui Zhang
  • Heng Li
  • Jie Xie
  • Jingquan Zhao
Regular Paper


Previously, it was clarified that phycobilisome (PBS) mobility and energy spillover were both involved in light-to-dark induced state transitions of intact Spirulina platensis cells. In this work, by taking advantage of the characteristic fluorescence spectra of photosystem I (PSI) trimers and monomers as indicators, the relative contributions for the “mobile PBS” and “energy spillover” are quantitatively estimated by separating the fluorescence contribution of PBS mobility from that of PSI oligomeric change. Above the phase transition temperature (T PT) of the membrane lipids, the relative proportion of the contributions is invariable with 65% of “mobile PBS” and 35% of “energy spillover”. Below T PT, the proportion for the “mobile PBS” becomes larger under lowering temperature even reaching 95% with 5% “energy spillover” at 0°C. It is known that lower temperature leads to a further light state due to a more reduced or oxidized PQ pool. Based on the current result, it can be deduced that disequilibrium of the redox state of the PQ pool will trigger PBS movement instead of change in the PSI oligomeric state.


Cyanobacterium State transition Phycobilisomes Mobility Energy spillover Relative contribution 













Photosystem I


Photosystem II




Phase-transition temperature



The research is supported by the National Natural Science Foundation of China (NSFC) (No. 30570422, 502211201, 90306013 and 3047037).


  1. Allen JF, Holmes NG (1986) A general model for regulation of photosynthetic unit function by protein phosphorylation. FEBS Lett 202:175–181CrossRefGoogle Scholar
  2. Barber J (1986) Regulation of energy transfer by cations protein phosphorylation in relation to thylakoid organization. Photosynth Res 10:243–253CrossRefGoogle Scholar
  3. Biggins J, Bruce D (1989) Regulation of excitation energy transfer in organisms containing phycobilins. Photosynth Res 20:1–34CrossRefGoogle Scholar
  4. Bonaventura C, Myers J (1969) Fluorescence and oxygen evolution from Chlorella pyrenoidosa. Biochim Biophys Acta 189:366–383PubMedCrossRefGoogle Scholar
  5. Bruce D, Brimble S, Bryant DA (1989) State transition in a phycobilisome-less mutant of the cyanobacterium Synechococcus sp. PCC 7002. Biochim Biophys Acta 974:66–73PubMedCrossRefGoogle Scholar
  6. Federman S, Malkin R, Scherz A (2000) Excitation energy transfer in aggregates of photosystem I and photosystem II of the cyanobacterium Synechocystis sp. PCC 6803: Can assembly of the pigment–protein complexes control the extent of spillover? Photosynth Res 64:199–207PubMedCrossRefGoogle Scholar
  7. Govindjee, Krogmann D (2005) Discoveries in oxygenic photosynthesis (1727–2003): a perspective. In: Govindjee, Beatty JT, Gest H, Allen JF (eds) Discoveries in photosynthesis. Advances in photosynthesis and respiration, vol 20. Springer, The Netherlands, pp 63–105CrossRefGoogle Scholar
  8. Govindjee, Satoh K (1986) Fluorescence properties of chlorophyll b- and chlorophyll c-containing algae. In: Govindjee, Amesz J, Fork DC (eds) Light emission by plants and bacteria. Academic Press, Orlando, pp 497–537Google Scholar
  9. Govindjee, Owens OVH, Hoch G (1963) A mass spectroscopic study of the emerson enhancement effect. Biochim Biophys Acta 75:281–284PubMedCrossRefGoogle Scholar
  10. Joshua S, Mullineaux CW (2004) Phycobilisome diffusion is required for light-state transitions in cyanobacteria. Plant Physiol 135:2112–2119PubMedCrossRefGoogle Scholar
  11. Karapetyan NV, Dorra D, Schweitzer G, Bezsmertnaya IN, Holzwarth AR (1997) Fluorescence spectroscopy of the longwave chlorophylls in trimeric and monomeric photosystem I core complexes from the cyanobacterium Spirulina platensis. Biochemistry 36:13830–13837PubMedCrossRefGoogle Scholar
  12. Karapetyan NV, Shubin VV, Strasser RJ (1999) Energy exchange between the chlorophyll antennae of monomeric subunits within the Photosystem I trimeric complex of the cyanobacterium Spirulina platensis. Photosynth Res 61:291–301CrossRefGoogle Scholar
  13. Li DH, Xie J, Zhao YW, Zhao JQ (2003) Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation. Biochim Biophys Acta 1557:35–40PubMedCrossRefGoogle Scholar
  14. Li DH, Xie J, Zhao JQ, Xia AD, Li D, Gong Y (2004) Light-induced excitation energy redistribution in Spirulina platensis cells “spillover” or “mobile PBSs”? Biochim Biophys Acta 1608:114–121PubMedCrossRefGoogle Scholar
  15. Li H, Li DH, Yang SZ, Xie J, Zhao JQ (2006) The state transition mechanism-simply depending on light-on and -off in Spirulina platensis. Biochim Biophys Acta 1757:1512–1519PubMedCrossRefGoogle Scholar
  16. Li H, Yang SZ, Xie J, Zhao JQ (2007a) Probing the connection of PBSs to the photosystems in Spirulina platensis by artificially induced fluorescence fluctuations. J Lumin 122–123:294–296CrossRefGoogle Scholar
  17. Li H, Yang SZ, Xie J, Feng J, Gong Y, Zhao JQ (2007b) The origin of the temperature-induced fluorescence fluctuation in Spirulina platensis: temperature-sensitive mobility of PQ molecules. Photosynth Res 94:59–65PubMedCrossRefGoogle Scholar
  18. Li Y, Zhang JP, Xie J, Zhao JQ, Jiang L (2001) Temperature-induced decoupling of phycobilisomes from reaction centers. Biochim Biophys Acta 1504:229–234PubMedCrossRefGoogle Scholar
  19. McConnell MD, Koop R, Vasilév S, Bruce D (2002) Regulation of the distribution of chlorophyll and phycobilin-absorbed excitation energy in cyanobacteria. A structure-based model for the light state transition. Plant Physiol 130:1201–1212PubMedCrossRefGoogle Scholar
  20. Mullineaux CW (1992) Excitation energy transfer from phybobilisomes to photosytem I in a cyanobacterium. Biochim Biophys Acta 1100:285–292Google Scholar
  21. Mullineaux CW, Allen JF (1990) State 1–State 2 transitions in the cyanobacterium Synechococcus 6301 are controlled by the redox state of electron carriers between Photosystem I and II. Photosynth Res 23:297–311CrossRefGoogle Scholar
  22. Mullineaux CW, Tobin MJ, Jones GR (1997) Mobility of photosynthetic complexes in thylakoid membranes. Nature 390:421–424CrossRefGoogle Scholar
  23. Murata N (1969) Control of excitation transfer in photosynthesis. Biochim Biophys Acta 172:242–251PubMedCrossRefGoogle Scholar
  24. Papageorgiou GC, Govindjee (2005) Chlorophyll a fluorescence: a bit of basics and history. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Advances in photosynthesis and respiration, vol 19. Springer, The Netherlands, pp 1–42Google Scholar
  25. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvent; verification of the concentration of chlorophyll standards by absorption spectroscopy. Biochim Biophys Acta 975:384–394CrossRefGoogle Scholar
  26. Rabinowitch E, Govindjee (1969) Photosynthesis. John Wiley and Sons Inc., NY, pp 34–36Google Scholar
  27. Rakhimberdieva MG, Boichenko VA, Karapetyan NV, Stadnichuk IN (2001) Interaction of phycobilisomes with photosystem II dimers and Photosystem I monomers and trimers in the cyanobacterium Spirulina platensis. Biochemistry 40:15780–15788PubMedCrossRefGoogle Scholar
  28. Shubin VV, Bezsmertnaya IN, Karapetyan NV, Mohanty P (1991) Origin of the 77 K variable fluorescence at 758 nm in the cyanobacterium Spirulina platensis. Biochim Biophy Acta 1060:28–36CrossRefGoogle Scholar
  29. Williams WP, Allen JF (1987) State 1/state 2 changes in higher plants and algae. Photosynth Res 13:19–45CrossRefGoogle Scholar
  30. Zarrouk C (1966) Contribution to the study of a cyanophycea: influence of various physical and chemical factors on the growth and photosynthesis of Spirulina maxima. Ph.D. Thesis, University of Paris, ParisGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of PhotochemistryInstitute of Chemistry, Chinese Academy of Sciences (CAS)BeijingP.R. China

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