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Photosynthesis Research

, Volume 94, Issue 2–3, pp 315–320 | Cite as

Estimation of relative contribution of “mobile phycobilisome” and “energy spillover” in the light–dark induced state transition in Spirulina platensis

  • Rui Zhang
  • Heng Li
  • Jie Xie
  • Jingquan Zhao
Regular Paper

Abstract

Previously, it was clarified that phycobilisome (PBS) mobility and energy spillover were both involved in light-to-dark induced state transitions of intact Spirulina platensis cells. In this work, by taking advantage of the characteristic fluorescence spectra of photosystem I (PSI) trimers and monomers as indicators, the relative contributions for the “mobile PBS” and “energy spillover” are quantitatively estimated by separating the fluorescence contribution of PBS mobility from that of PSI oligomeric change. Above the phase transition temperature (T PT) of the membrane lipids, the relative proportion of the contributions is invariable with 65% of “mobile PBS” and 35% of “energy spillover”. Below T PT, the proportion for the “mobile PBS” becomes larger under lowering temperature even reaching 95% with 5% “energy spillover” at 0°C. It is known that lower temperature leads to a further light state due to a more reduced or oxidized PQ pool. Based on the current result, it can be deduced that disequilibrium of the redox state of the PQ pool will trigger PBS movement instead of change in the PSI oligomeric state.

Keywords

Cyanobacterium State transition Phycobilisomes Mobility Energy spillover Relative contribution 

Abbreviations

APC

Allophycocyanin

C-PC

C-phycocyanin

Chl

Chlorophyll

DCMU

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

PBS

Phycobilisome

PSI

Photosystem I

PSII

Photosystem II

PQ

Plastoquinone

TPT

Phase-transition temperature

Notes

Acknowledgements

The research is supported by the National Natural Science Foundation of China (NSFC) (No. 30570422, 502211201, 90306013 and 3047037).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of PhotochemistryInstitute of Chemistry, Chinese Academy of Sciences (CAS)BeijingP.R. China

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