Photosynthesis Research

, Volume 84, Issue 1–3, pp 209–215 | Cite as

Kinetics of delayed chlorophyll a fluorescence registered in milliseconds time range

  • V. Goltsev
  • P. Chernev
  • I. Zaharieva
  • P. Lambrev
  • R. J. Strasser


Delayed fluorescence dark decays in the time interval from 0.35 to 5.5ms are measured during dark to light adaptation in whole barley leaves and isolated thylakoid membranes, using a disc phosphoroscope. The changes in delayed fluorescence features are compared with variable chlorophyll fluorescence simultaneously registered with the same apparatus as well as in parallel by Handy PEA (Hansatech Instruments Ltd.), and absorbance changes at 820 nm. The registered delayed fluorescence signal is a sum of three components – submillisecond with lifetime of about 0.6 ms, millisecond decayed 2–4 ms and slow component with lifetime > >5.5 ms. The submillisecond delayed fluorescence component is proposed to be a result of radiative charge recombination in Photosystem II reaction centers in the state Z+PQ A Q B , and its lifetime is determined by the rate of electron transfer from Q A to Q B . The millisecond delayed fluorescence component is associated with recombination in Z+PQ A Q B = centers with a lifetime determined by the sum of the rate constants of electron transfer from the oxygen-evolving complex to Z+ and of the exchange between the reduced and oxidized plastoquinone pool in the Q B -site. On the basis of these assumptions and of the different share of the three components in the integral delayed fluorescence during induction, an attempt has been made to interpret the changes in the delayed fluorescence intensity during the transition of the photosynthetic apparatus from dark to light adapted state.


dark relaxation kinetics electron transport millisecond delayed fluorescence Photosystem II variable chlorophyll fluorescence 



delayed chlorophyll fluorescence


prompt (variable) chlorophyll fluorescence






reaction center


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Crofts, AR, Bartoli, I, Kramer, D, Taoka, S 1993Kinetics of electron transfer between Q A and Q B in wild-type and herbicide-resistant mutants of C. rheinhardtiiZ Naturforsch48259266Google Scholar
  2. Duysens LNM and Sweers HE (1963) Mechanism of two photochemical reactions in algae as studied by means of fluorescence. In: Japanese Society of Plant Physiologists (eds) Studies on Microalgae and Photosynthetic Bacteria, pp 353–372. University of Tokyo Press, TokyoGoogle Scholar
  3. Goltsev, VN, Yordanov, IT 1997Mathematical model of prompt and delayed chlorophyll fluorescence induction kineticsPhotosynthetica33571586Google Scholar
  4. Jursinic, P 1986Delayed fluorescence: current concepts and statusGovindjee, Amesz, JFork, DJ eds. Light Emission by Plants and BacteriaAcademic PressOrlando, Florida291328Google Scholar
  5. Jursinic, P, Govindjee, , Wraight, CA 1978Membrane potential and microsecond to millisecond delayed light emission after a single excitation flash in isolated chloroplastsPhotochem Photobiol276171Google Scholar
  6. Lavorel, J 1975LuminescenceGovindjee,  eds. Bioenergetics of PhotosynthesisAcademic PressNew York223317Google Scholar
  7. Lazar, D 1999Chlorophyll a fluorescence inductionBiochim Biophys Acta1412128PubMedGoogle Scholar
  8. Rutherford, AW, Govindjee, , Inoue, Y 1984Charge accumulation and photochemistry in leaves studied by thermoluminescence and delayed light emissionProg Natl Acad Sci USA8111071111Google Scholar
  9. Satoh, K, Katoh, S 1983Induction kinetics of millisecond-delayed luminescence in intact Bryopsis chloroplastsPlant Cell Physiol24953962Google Scholar
  10. Strasser RJ, Schansker G, Srivastava A and Govindjee (2001) Simultaneous measurement of Photosystem I and Photosystem II probed by modulated transmission at 820 nm and by chlorophyll a fluorescence in the sub ms to second time range. In: PS2001 Proceedings: 12th International Congress on Photosynthesis. CSIRO Publishing, Melbourne, Australia.Google Scholar
  11. Van Gorkom, HJ, Donze, M 1973Charge accumulation in the reaction center of Photosystem 2Photochem Photobiol17333342Google Scholar
  12. Whatley, FR, Arnon, DI 1963Photosynthetic phosphorylation in plantsColowick, SPKaplan, NO eds. Methods in EnzimologyAcademic PressNew York308313Google Scholar
  13. Wraight, CA, Crofts, AT 1971Delayed fluorescence and the high-energy state of chloroplastsEur J Biochem19386397PubMedGoogle Scholar
  14. Zaharieva, I, Goltsev, V 2003Advances on Photosystem II investigation by measurement of delayed chlorophyll fluorescence by a phosphoroscopic methodPhotochem Photobiol77292298PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • V. Goltsev
    • 1
  • P. Chernev
    • 1
  • I. Zaharieva
    • 1
  • P. Lambrev
    • 2
  • R. J. Strasser
    • 3
  1. 1.Department of Biophysics and Radiobiology, Biological Faculty, St. Kliment OhridskiUniversity of SofiaSofiaBulgaria
  2. 2.Institute of BiophysicsBulgarian Academy of SciencesSofiaBulgaria
  3. 3.Bioenergetics LaboratoryUniversity of GenevaSwitzerland

Personalised recommendations