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Photosynthesis Research

, Volume 84, Issue 1–3, pp 131–137 | Cite as

A thermoluminescence study of Photosystem II back electron transfer reactions in rice leaves – effects of salt stress

  • Jorge L. Zurita
  • Mercedes Roncel
  • Manuel Aguilar
  • José M. Ortega
Regular paper

Abstract

The recombination reactions of Photosystem II have been investigated in vivo in rice leaves by using the thermoluminescence (TL) emission technique. Excitation of dark-adapted leaf segments at 0 °C with different number of single turn-over flashes induced the appearance of complex TL glow curves. The mathematical analysis of these curves showed the existence of four TL components: B1-band (temperature maximum, tmax, at 24 °C, originating from S3Q B recombination), B2-band (tmax at 35 °C, from S2Q B ), AG-band (tmax at 46 °C) and C-band (tmax at 55 °C, from Tyr D + Q A ). Their contributions to the total TL signal were different depending on the number of flashes given. AG-band seems to reflect a special electron transfer from some unknown stroma donor to PS II. Q-band (tmax at 19 °C), originating from S2Q A recombination, was recorded after flashing samples incubated in the presence of DCMU. The recombination halftimes (t1/2) at 20 °C of S2Q A , S3Q B , S2Q B and Tyr D + Q A were, respectively, 0.8 s, 48 s, 74 s and about 1 h. A sharp AG-band (tmax at 50 °C and t1/2 of 210 s) could be also observed after illumination of leaves with far-red light and after a dark incubation period of whole plants. Incubation of leaf segments with 0.5 M NaCl abolished the inductions of AG-band by darkness and far-red illumination, significantly decreased Q-band intensity, whereas induced a strong increase in C-band intensity. The possible inhibition of S2/S3 formation and quinone oxidation by saline stress are discussed.

Keywords

afterglow Photosystem II recombination reactions rice salt stress thermoluminescence 

Abbreviations

AG

afterglow (luminescence bounce)

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

OEC

oxygen evolving complex

PS I

Photosystem I

PS II

Photosystem II

QA and QB

the primary and secondary quinone electron acceptor in the reaction center of PS II

S0... 4

redox states of water oxidation complex

TL

thermoluminescence

tmax

temperature of the maximum of a TL band

TyrD

tyrosine 160 residue of D2 protein of PS II

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Copyright information

© Springer 2005

Authors and Affiliations

  • Jorge L. Zurita
    • 1
  • Mercedes Roncel
    • 1
  • Manuel Aguilar
    • 2
  • José M. Ortega
    • 1
  1. 1.Instituto de Bioquímica Vegetal y FotosíntesisUniversidad de Sevilla-CSICSevillaSpain
  2. 2.Junta de AndalucíaCIFA Las Torres y TomejilAlcalá del RíoSpain

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