Global Analysis of Gene Expression Profiles in Glutinous Rice 89-1 (Oryza sativa L.) Seedlings Exposed to Chilling Stress

Abstract

Rice (Oryza sativa) is a tropical cereal crop that is severely affected by chilling stress at the seedling stage, although glutinous rice 89-1 (Gr89-1) in Chongqing, China, shows tolerance to low temperatures and overwintering ability. However, little research has been conducted on the mechanisms regulating chilling stress in Gr89-1. In this study, a comprehensive of transcriptional profiles of Gr89-1 seedlings at the three-leaf stage was conducted after a 4 °C treatment for 2, 6, 12, 24, or 48 h. Overall, 2993 differentially expressed genes were detected in Gr89-1 seedlings upon cold exposure. Gene Ontology testing and pathway analysis revealed differentially expressed genes involved in transcriptional regulation, carbohydrate metabolism, plant hormone signal, and cell wall composition. A total of 243 transcription factors were differentially expressed during the cold treatment; in particular, the AP2/EREBP, bHLH, NAC, WRKY, C2H2, and TIFY families were generally upregulated after cold treatment, whereas the mTERF and GNAT families were downregulated. Chilling stress changed the starch and sucrose metabolism, coupled with the accumulation of sucrose and trehalose level, and increases in jasmonic acid level in Gr89-1 seedlings. Furthermore, a number of the cell wall-related genes identified in the present study were also differentially expressed during the cold treatment. The genes and pathways identified in the current study increase our understanding of the mechanisms underlying cold resistance in rice seedlings.

Introduction

Chilling (0–15 °C) stress severely affects rice growth and development in temperate and sub-tropical areas. Rice seedlings during early spring in such areas are often subjected to low temperatures, leading to poor growth and reduced yield (Baruah et al. 2009). Most temperate plants have evolved regulatory mechanisms to adapt to unfavorable low-temperature environments through a complex process known as cold acclimation (Thomashow 1999). The C-repeat binding factor/dehydration-responsive element-binding protein 1 (CBF/DREB1; DREB1A, DREB1B, and DREB1C)-dependent response pathway plays a vital role in cold tolerance during cold acclimation (Chinnusamy et al. 2007; Kim 2007; Shinozaki et al. 2003). CBFs mediate cold-responsive genes with various functions, including transcription, osmoprotectant biosynthesis, and signal transduction events (e.g., plant hormone signaling), among many others (Krasensky et al. 2012; Lissarre et al. 2010).

The amount of compatible solutes (e.g., amino acids, amines, and carbohydrates) is particularly important in the stabilization of membranes and associated with the chilling tolerance (Krasensky and Jonak 2012). Many studies have found that soluble carbohydrate, sucrose, trehalose, and stachyose drastically accumulated in plants under low temperatures (Krasensky and Jonak 2012). Phytohormones regulate multiple aspects of plant growth and development, and also play substantial direct or indirect roles in plant responses to diverse stressors (Jeon and Kim 2013). Jasmonic acid (JA) represents an important class of lipid-derived plant hormones involved in the cold response (Peleg and Blumwald 2011). Endogenous free JA accumulates during low-temperature conditions in camellias and wheat (Li et al. 2016; Qian et al. 2016). Methyl jasmonate treatment increases the chilling tolerance of banana, pomegranate fruit, and wheat seedlings (Qi et al. 2006; Zhao et al. 2013; Zolfagharinasab and Hadian 2007). The catalytic steps in JA biosynthesis involving the conversion of α-linolenic acid to JA are mediated by the action of enzymes encoded by the lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC), 12-oxophytodienoic acid reductase (OPR), and β-oxidase genes (Wasternack and Hause 2013). After synthesis, the COI1-JAZ co-receptor perceives bioactive JA-Ile. As a negative regulator of the JA signaling pathway, the JASMONATE-ZIM-DOMAIN (JAZ) protein regulates the expression of JA-responsive genes by inhibiting the activities of a series of downstream transcription factors (TFs) (Hu et al. 2017). JA acts as a signal in the ICE-CBF pathway, which is involved in cold tolerance in Arabidopsis and tomato (Hu et al. 2013; Wang et al. 2016). However, the integrative regulatory effects of JA in rice subject to cold are poorly understood.

Transcriptomic analyses have provided new insight into the complex stress response mechanisms of plants. A large number of genome-wide gene profiling studies on rice responding to low temperature have been conducted. Most of the cold-responsive genes identified are related to transcription regulation, osmoprotectant accumulation, detoxification, ROS scavenging, primary energy metabolism, cell wall organization, and hormone-related signal transduction processes (Buti et al. 2018; Chawade et al. 2013; Pradhan et al. 2019; Zhang et al. 2012ab; Zhang et al. 2017a, b). Glutinous rice 89-1 (Gr89-1) has overwintering ability and germinates through axillary buds during the following spring. Yields are the same as in a normal season (up to 6.29 t/hm2) in Chongqing, China (Deng et al. 2018). Other studies have revealed similar overwintering ability of Oryza longistaminata and Dongxiang wild rice (O. rufipogon) in south and southwest China (He et al. 1996; Liang et al. 2017; Mao et al. 2015; Zhang et al. 2017a, b). Genome-wide gene profiling has shown that genes associated with CBF/DREB1 regulon transcriptional regulation are significantly upregulated in wild rice (O. longistaminata) shoots in response to long-term low temperatures (Zhang et al. 2017a, b). In a previous study, Gr89-1 had better seedling-stage chilling tolerance according to a chlorophyll fluorescence analysis (Wang et al. 2012). Gr89-1 has clearly developed methods to protect itself against chilling stress, and genome-wide gene profiling provides a valuable method for understanding the molecular mechanisms underlying the low temperature response. In the present study, we analyzed transcriptomic profile changes in Gr89-1 seedlings exposed to a cold treatment (4 °C for 2, 6, 12, 24, or 48 h) using a deep high-throughput sequencing-based digital gene expression analysis. Gene Ontology (GO) testing, and combined biological pathway and physiological data analysis, indicated that Gr89-1 improves its response to chilling stress by increasing the production of osmoprotectants (soluble sugar, sucrose, and trehalose), modifying its cell wall structure and composition, and altering the JA biosynthetic and signal transduction pathways.

Methods

Plant Growth and Experimental Treatment

The rice (Oryza sativa, ssp. japonica) cultivar ‘Gr89-1’, which was originally introduced by local farmers in Zhongxian County and domesticated at the Chongqing Academy of Agricultural Sciences, was used in this study. The seeds of Gr89-1 and WH86 (O. sativa, ssp. indica) were soaked in water for 24 h at room temperature, and then transplanted to plastic plates with Yoshida’s culture solution (Yoshida et al. 1972) in a growth chamber (3 M, Neuss, Germany) with a daily temperature of 26 °C and a long-day conditions (14 h light/10 h dark). The three-leaf seedlings on plates were transferred to the growth chamber under 24-h illuminations and maintained at 4 ± 1 °C for the cold treatment. Leaf samples were collected from 25 seedlings at each time point (0, 2, 6, 12, 24, and 48 h) and repeated three times. The prepared samples were frozen in liquid nitrogen and stored at − 80 °C.

RNA Isolation, Library Construction, and Bioinformatics Analysis

Total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and DNA was removed using RNase-free DNase I (Roche, Indianapolis, IN, USA). The Nanodrop ND-1000 (Thermo Scientific, Waltham, MA, USA) and Agilent 2100 instruments (Agilent Technologies, Palo Alto, CA, USA) were used to detect RNA quality and concentration, respectively.

Two biological replicates of the Gr89-1 seedlings sampled at the six time points were sequenced to generate 12 sequencing samples, and sequencing was performed by BGI-Shenzhen on the Illumina HiSeq™ 2000 platform, generating a total of million paired-end reads of bp length. Prior to assembly, the low-quality reads were removed. After that, a total of G bp of cleaned and paired-end reads was produced. All the sequencing saturation data are shown in Supplementary Table S1. The raw sequence data have been submitted to the NCBI Short Read Archive (SRA) with accession number PRJNA558598.

We aligned the 125-bp paired-end reads to the rice reference genome provided by the Rice Gene Annotation Project (http://rice.plantbiology.msu.edu/) at Michigan State University using TopHat (v2.0.6) (Ouyang et al. 2007; Trapnell et al. 2009), and then, unique transcript sequences of each sample were assembled using the Cuffdiff tool in the Cufflinks package (http://cole-trapnell-lab.github.io/cufflinks/) (Trapnell et al. 2012). Then, the assembled transcripts from each sample were compared with the reference annotation to reconstruct a non-redundant transcript data set using Cuffcompare. After the final transcriptome was generated, Cuffdiff was used to estimate the expression levels of all transcripts (Romualdi et al. 2003). Up- or downregulated genes under cold stress were selected with ∣log2Ratio∣ ≥ 2, p value < 0.05, and FDR < 0.001. A GO analysis was performed, with agriGO (http://bioinfo.cau.edu.cn/agriGO/index.php) used to identify the significant terms (q ≤ 0.05). Also, a metabolic pathway analysis was conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG; http://www.genome.jp/kegg/pathway.html) database.

qRT-PCR Analysis

To validate the transcriptomic data, we reverse-transcribed 2 mg total RNA per sample into first-strand cDNA with M-MLV reverse transcriptase (Promega, Madison WI, USA) and performed quantitative real-time polymerase chain reaction (qRT-PCR) on the BIO-RADCFX96 Real Time System (Bio-Rad Laboratories, Hercules, CA, USA) using a SYBR green PCR kit (Code: DRR041A; TaKaRa Bio, Shiga, Japan). Each sample had three technical replicates. To quantify the relative transcript levels of each gene, we applied the cycle threshold (ΔΔCT) method and referred to the transcript level of OsAct1 (LOC_Os05g36290). The primer sequences are listed in Supplementary Table S2.

Measurement of Chlorophyll Fluorescence

We used MONITORING-PAM (Heinz Walz GmbH) to measure chlorophyll fluorescence and estimated the photosystem II efficiencies Fv/Fm = (Fm-F0)/Fm. Twenty-five seedlings each from Gr89-1 and WH86 treated with chilling stress for 48 h were sent to the fluorometer for records at 0, 2, 6, 12, 24, and 48 h, with three replicates for each sample. Before measurement, plants went through a 1-h dark acclimation. All measurements were conducted at 10 pm.

Chemical Substance Assays

The JA contents of the seedlings at 0, 2, 6, 12, 24, and 48 h under the 4 °C condition were determined following the Liu’s procedure (Liu et al. 2018). The prepared samples were analyzed using a 4000 Q-Trap high-performance liquid chromatography-mass spectrometry (HPLC-MS) system (AB SCIEX, Framingham, MA, USA), and 10-dihydro-JA (Olchemin) for JA was used as internal standards. The contents of free proline, total soluble sugar, sucrose, and trehalose in the seedlings subjected to cold stress were determined using kits from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All experiments included three biological repeats.

Results and Discussion

Physiological Indices Indicate that Gr89-1 Exhibits Higher Cold Tolerance than WH86

To verify that Gr89-1 possesses higher cold tolerance than WH86, we treated three-leaf seedlings at 4 °C for 48 h, followed by a 1-week recovery period under normal growth conditions. Wilting and death occurred in all WH86 plants, while the recovery rate of Gr89-1 plants was 80% (Fig. 1). Prior to cold exposure, there was no difference in the Fv/Fm ratios (0.76 and 0.758) of Gr89-1 and WH86. After 24 h of cold treatment, the Fv/Fm ratio dropped to 0.12 in WH86 and to 0.622 in Gr89-1. After 48 h, the ratio was almost zero in WH86, versus 0.456 in Gr89-1 (Fig. 1c). These results demonstrate that Gr89-1 adapted more rapidly than WH86 in response to the low temperature treatment.

Fig. 1
figure1

taken from dark acclimated plants at the described time-points

Chilling stress survival in Gr89-1 and WH86. Seedlings of WH86 and Gr89-1 were grown to three-leaf stage under regular growth conditions (see Materials and Methods) a and then moved to 4 °C (cold conditions) for 48 h and thereafter allowed to recover for 1 week b. c Chlorophyll fluorescence measurements. Three-leaf stage plants were used for the analysis. Plants were dark acclimated for 1 h and then moved to + 4 °C. Chlorophyll fluorescence measurements were

Transcriptome Profiling in Response to Chilling Stress in Seedlings

To study cold-induced changes in gene expression of the cold-tolerant genotype, the transcriptomes of Gr89-1 at six different cold treatment time points (0, 2, 6, 12, 24, and 48 h) were studied using the Illumina sequencing platform. The main features of these six libraries are summarized in Supplementary Table S3. For each library, 437,609 distinct tag sequences were generated (~ 12 million sequence tags in total). After removing the low-quality reads and trimming the adapter sequences, we obtained 221,840 distinct clean tag sequences (~ 11.7 million total clean sequence tags for each library) (Supplementary Table S3). Using Trinity for the transcriptome assembly, we unambiguously mapped 32.89–36.46% of the distinct clean tags to the UniGene database and 22.66–29.66% to the rice genome database; 15.21–21.83% of the distinct clean tags could not be mapped to the UniGene virtual tag database (Supplementary Table S3).

Genes presenting more than fourfold expression changes relative to the control level were identified as DEGs. In total, we identified 557, 1089, 1325, 1867, and 2342 DEGs at 2, 6, 12, 24, and 48 h, respectively, after chilling stress (Fig. 2b and Supplementary Table S4); 342 DEGs were significantly regulated at all five time points, and 16, 84, 118, 212, and 683 DEGs were present at 2, 6, 12, 24, and 48 h after chilling stress, respectively (Fig. 2a).

Fig. 2
figure2

Venn diagram and histogram of DEGs in Gr89-1 under chilling stress conditions. a Venn diagram showing DEGs expressed at each of the five sampling time points during cold stress. b The number of genes up- or down-regulated at different time points during cold stress

To explore the potential functions of the DEGs, functional annotation GO analysis was performed. All 2993 DEGs were divided into three categories (cellular components, molecular functions, and biological processes). The majority of the DEGs annotated as cellular components were categorized into cell part (GO: 0044464), organelle (GO: 0043226), and nuclear (GO: 0005634) subgroups. The majority of the DEGs annotated as molecular functions were categorized into catalytic activity (GO: 0003824), binding (GO: 0005488), and transcriptional regulatory activity (GO: 0030528) subgroups. The DEGs assigned to the catalytic activity subgroup mainly showed transferase, hydrolase, kinase, and oxidoreductase activities. The most common substances in the subgroups were carbohydrate, nucleosides, ions, tetrapyrroles, hemes, and proteins. The DEGs annotated as biological processes were mainly categorized into metabolic (GO: 0008152), cellular (GO: 0009987), and regulation of biological process (GO: 0050789) subgroups. The transport (GO: 0006810) and transcription (GO: 0006350) terms were also enriched within the biological processes category (Fig. 3). The phenylpropanoid biosynthesis (ko00940), plant hormone signal transduction (ko04075), protein processing in endoplasmic reticulum (ko04141), glutathione metabolism (ko00480), MAPK signaling pathway (ko04016), and starch and sucrose metabolism (ko00500) were significantly affected during low temperature (Fig. 4).

Fig. 3
figure3

Gene Ontology (GO) classifications of the DEGs in Gr89-1 under chilling stress conditions. Genes with unknown function is not included. Bars show numbers of cold responded genes in the Gr89-1(p ≤ 0.05)

Fig. 4
figure4

KEGG enrichment analyses of all DEGs in Gr89-1 under chilling stress conditions. The Y-axis indicates the number of DEGs that were assigned to a specific pathway. The X-axis indicates the KEGG pathways

Chilling Stress Responsive Genes in Gr89-1 Seedlings

JA Metabolism and Signaling-Related Genes

In this study, 82 genes were involved in different plant hormone biosynthetic and signal transduction pathways during chilling stress, including jasmonic acid(JA), brassinosteroids (BRs), gibberellins (GAs), ABA, cytokinin (CK), ethylene (ET), salicylic acid (SA), and auxin (Figs. 5 and 6, Supplementary Table S5). These data indicated that a collaboration between phytohormone biosynthesis and signal transduction might be involved in the Gr89-1 seedlings during chilling tolerance. Thirteen DEGs were annotated as JA metabolism or signal transduction terms, including three LOXs (LOC_Os03g52860, LOC_Os04g37430, and LOC_Os08g39840) and three OPRs (LOC_Os06g11290, LOC_Os06g11260, and LOC_Os06g11210). Notably, almost all of the biosynthesis of JA genes was upregulated during cold acclimation (Fig. 5a and b, and Supplementary Table S5). Five JAZs (LOC_Os03g08310, LOC_Os03g08320, LOC_Os03g08330, LOC_Os10g25230, and LOC_Os10g25290) involved in the JA signaling pathway were upregulated during chilling stress in Gr89-1. The qRT-PCR results also showed that the LOXs, OPRs, and JAZs were strongly upregulated in rice seedlings (Fig. 5d and Supplementary Table S5). The transcriptional data suggested that JA accumulated under cold stress.

Fig. 5
figure5

Overview of putative JA biosynthesis pathways involved in rice and expression profiles of genes involved in this pathway. a Expression of genes involved in JA metabolism and signaling under low temperature conditions. b Biosynthetic pathway of JA in rice. Genes that are up- or downregulated by > 2-fold after stress are indicated in red and blue, respectively. c JA contents in Gr89-1 and WH86 leaves determined by HPLC-MS. d Relative expression of genes involved in JA metabolism pathways. TPM: number of transcripts per million tags. Error bars indicate the SD for three independent replicates. LOX, lipoxygenase; OPR, 12-OPDA, (15Z)-12-Oxophytodienoic acid; OPC-8, 3-oxo-2-(2-pentenyl)-cyclopentane-1-octanoic acid; JMT, S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase; 12-oxophytodienoate reductase; MeJA, methyl-JA; TIFY, ZIM domain-containing protein

Fig. 6
figure6

DEG enrichment of the plant hormone signal transduction pathway by KEGG annotation. The key regulatory components in multiple hormone response pathways are presented as their names (red, upregulated; green, downregulated). Gene IDs and fold changes in transcript abundance are indicated in Supplementary Table S5

Therefore, we measured the content of endogenous JA in Gr89-1 and WH86 seedlings by HPLC-MS in this study. It was worth noting that the content of JA was higher in Gr89-1 compared with WH86 under cold stress. Moreover, Gr89-1 showed a more sharply increased JA concentration after 24 h of cold treatment compared with WH86 (Fig. 5c). These results show that low temperature stress may influence the homeostasis of endogenous JA in rice seedlings, and the higher concentration of JA was detected in Gr89-1. These results show that activation of the JA biosynthetic pathway plays a key role in the cold tolerance of Gr89-1. The HPLC-MS and qRT-PCR results confirmed the reliability of the digital expression data.

Other Plant Hormones in Chilling Stress

In addition to JA, 69 genes were involved in different plant hormone biosynthetic and signal transduction pathways, including brassinosteroids (BRs), gibberellins (GAs), ABA, cytokinin (CK), ethylene (ET), salicylic acid (SA), and auxin (Supplementary Table S5). The DEGs for ET biosynthesis and signal transduction were upregulated. The expression of phenylalanine ammonia-lyase (PAL; LOC_Os02g41650 and LOC_Os04g43760), encoding an SA synthetic enzyme, was induced and the cytoplasmic binding protein non-expressor of pathogenesis-related genes (NPR1, LOC_Os03g46440) was upregulated. The PR-1 gene (LOC_Os10g11500), which encodes downstream proteins of the SA signaling cascade, was also upregulated at 2 h. BR, GA, ABA, CK, and auxin also participated in the chilling stress (Fig. 6 and Supplementary Table S5).

Protein Kinases

In seedlings, the DEGs involved in calcium signaling included calmodulin (CML), calcineurin B-like protein-interacting protein kinases, calcium-dependent protein kinases (CDPKs), and mitogen-activated kinases (Fig. 7 and Supplementary Table S6). It is worth emphasizing that the expression of all of CML, MAPK, MAPKK, and MAPKKK genes was upregulated at all five cold exposure (4 °C) time points, particularly LOC_Os02g58520, LOC_Os12g01400, LOC_Os01g50400, and LOC_Os05g46760, which displayed an at least 5.09-fold increase at all time points (Fig. 7 and Supplementary Table S6).

Fig. 7
figure7

Transcript profiling of calmodulin-like (CML), mitogen-activated protein kinase (MAPK), mitogen-activated protein kinase kinase (MAPKK), mitogen-activated protein kinase kinase kinase (MAPKKK) genes after 48 h of chilling stress

Transcription Factor-Related Genes

In total, 243 TF genes in Gr89-1 were identified during at least one chilling stress time point (Fig. 8 and Supplementary Table S7). We found 35 differentially regulated AP2/EREBP genes after 2 h of cold exposure, of which 25 were upregulated strongly. In particular, DREB1A (LOC_Os09g35030), DREB1B (LOC_Os09g35010), DREB1C (LOC_Os06g03670.1), DREB1F (LOC_Os01g73770), DREB1G (LOC_Os02g45450), DREB1H (LOC_Os09g35020), OsEATB (LOC_Os09g28440), and EREBP2 (LOC_Os01g64790) were highly upregulated in Gr89-1. A total of 109 TF genes from the MYB, NAC, bHLH, WRKY, C2H2, HB, and bZIP families were differentially expressed in seedlings, mostly after 2 h of cold exposure, whereas downregulation of all GNAT and mTERF family genes occurred, where the roles of these genes in chilling stress are unclear (Fig. 8 and Supplementary Table S7).

Fig. 8
figure8

Differentially expressed transcription factor (TF) encoding genes. TF encoding genes were identified from the Plant Transcription Factor database (PlantTFDB)

Osmotic Adjustment-Related Genes

Changes in osmoprotectants represent a typical physiological and biochemical response to stress. Genes involved in soluble sugar metabolism exhibited differential expression upon exposure to low temperature (Fig. 9 and Supplementary Table S8). Genes encoding β-amylase (LOC_Os10g41550), sucrose-phosphate synthase (LOC_Os02g09170), and sucrose synthase (LOC_Os04g24430) (5.7-fold after 2 h at 4 °C) were specifically upregulated in Gr89-1 under the chilling stress treatment. Several genes related to trehalose and stachyose biosynthesis were upregulated (Fig. 9 and Supplementary Table S8). Among the genes related to trehalose biosynthesis, two trehalose-6-phosphate synthases (TPS; LOC_Os08g34580 and LOC_Os08g31980), and one trehalose-6-phosphate phosphatase (LOC_Os08g31630) were upregulated in Gr89-1. Additionally, one stachyose synthase (LOC_Os01g07530) involved in stachyose biosynthesis was upregulated under chilling stress in Gr89-1 seedlings (Fig. 9 and Supplementary Table S8). Therefore, we measured whether the total soluble sugar, sucrose, and trehalose were also increased during the cold acclimation process (Fig. 9b). The quantitative determination of oligosaccharides analysis showed that the total soluble sugar contents increased sharply after 6 h of chilling stress and was maintained at a high level from 6 to 48 h compared with control seedlings (Fig. 2b). The sucrose level increased gradually during cold treatment and increased accumulation of trehalose after 12 h of chilling stress (Fig. 9b). These data indicate that the total soluble sugar, sucrose, and trehalose play vital roles in the cold resistance of Gr89-1.

Fig. 9
figure9

Starch and sucrose metabolisms in Gr89-1 seedlings subject to chilling stresses. a Expression of genes involved in starch and sucrose metabolism under low temperature conditions. b The total soluble sugar, sucrose, and trehalose contents in the seedling of Gr89-1 under normal temperature and cold treatments

Cell Wall-Related Genes

The genes related to the cell wall showing altered expression patterns were divided into precursor generation, polysaccharide synthesis, and assembly subgroups (Fig. 10 and Supplementary Table S9). During precursor synthesis, the enzyme UDP-glucuronate 4-epimerase, which catalyzes the transformation of UDP-α-glucose to UDP-α-galactose, and LOC_Os01g62020, LOC_Os02g54890, and LOC_Os08g41440, were upregulated after 2 h of chilling stress. Among the polysaccharide synthesis-related genes, two genes for hemicellulose synthase (CSLC-9: LOC_Os03g56060; GT47: LOC_Os03g20850) and four genes encoding pectin biosynthetic (GT8: LOC_Os02g50600, LOC_Os03g21250, LOC_Os03g47530, and LOC_Os08g23780) genes were activated in seedlings. Notably, the expression of the transcript for LOC_Os03g47530 was over sevenfold higher at the beginning of exposure to 4 °C. We observed that several genes involved in cell wall assembly were activated and/or repressed in seedlings under cold stress. Five genes in the expansin (EXP) family were downregulated under cold stress. Among the nine DEGs in the xyloglucan endotransglucosylases/hydrolase (XTH) family, most were upregulated in rice seedlings under cold stress. The 19 glycosyl hydrolase (GH) family genes identified were mainly from GH17; LOC_Os01g71860 and LOC_Os06g34020 showed an at least 4.09-fold increase after 2 h of cold exposure. The esterase LOC_Os03g18860 was upregulated 9.64-fold after 48 h at 4 °C. Similarly, LOC_Os10g05820, which belongs to the proline-rich protein (PRP) gene family, also showed strongly increased expression after 6 h of cold treatment (Fig. 10 and Supplementary Table S9).

Fig. 10
figure10

List of cell wall-related genes after 48 h of chilling stress

Discussion

Rice is one of the most important food crops in China. Low temperature is a major factor affecting rice geographical distribution growth, development, quality, and productivity. It is significant to study the molecular mechanism of low-temperature tolerance of rice and cultivate high-yield and high-quality cold-resistant varieties to ensure food security in China. In this study, Gr89-1, which tolerates low temperatures and has overwintering ability, was used to investigate the molecular mechanism of rice response to chilling stress at seedling stage by RNA-seq technology. After comprehensive analysis of DEGs, we inferred that JA biosynthetic and signal transduction, sucrose, stachyose, and trehalose synthesis, and cell structure and synthesis were particularly influenced in Gr89-1 seedlings under cold stress.

Phytohormones, such as ABA, GA, SA, auxin, and ET, are positive or negative regulators of the chilling stress response (Miura and Furumoto 2013). In this study, collaboration between phytohormone biosynthesis and signal transduction might be involved in the Gr89-1 seedlings during chilling tolerance (Figs. 5 and 6, Supplementary Table S5). The phytohormone JA regulates plant responses to biotic and abiotic stressors (Wasternack and Hause 2013). Exogenous application of MeJA increases the chilling tolerance in Arabidopsis, banana, pomegranate fruit, and wheat (Hu et al. 2013; Qi et al. 2006; Zhao et al. 2013; Zolfagharinasab and Hadian 2007). Exposure to cold rapidly accumulates endogenous JA content by inducing JA biosynthesis genes such as LOX1, AOS1, AOC1, AOC2, and JAR1 in Arabidopsis (Goulas et al. 2006; Hu et al. 2013) and OsAOS, OsOPR1, OsAOC, and OsLOX2 in rice (Du et al. 2013). Compared with wild-type plants, Arabidopsis mutants (lox2, aos, jar1, and coi1) deficient in JA biosynthesis or signaling are more sensitive to freezing stress (Hu et al. 2013). In C. japonica, nine JA biosynthesis genes are induced during cold acclimation (Li et al. 2016). Similarly, the expression profiles of our six JA-biosynthetic genes (three LOXs: LOC_Os03g52860, LOC_Os04g37430, and LOC_Os08g39840, and three OPRs: LOC_Os06g11290, LOC_Os06g11260, and LOC_Os06g11210) indicated significant upregulation during cold exposure in this study, and free JA content was upregulated in 4 °C-treated seedlings of Gr89-1 after 24 h (Fig. 5c). It has also reported that the Ca2+ and MAPK cascades are also involved in the regulation of JA biosynthesis (Wasternack and Hause 2013). CDPKs act as positive regulators of plant Ca2+-regulated cold signaling (Saijo et al. 2010). The induction of CML genes by chilling stress has been described in wild rice (Zhang et al. 2017a, b). In our study, 12 CDPK and 8 CML genes were differentially expressed under severe chilling stress, and most of these genes were activated (Fig. 7 and Supplementary Table S6). The mitogen-activated protein kinase (MAPK) cascade is implicated in the regulation of cold signaling and freezing tolerance in plants (Teige et al. 2004). In this study, 12 MKKK, 2 MKK, and 2 MAPK genes showed increased expression during cold exposure (Fig. 7 and Supplementary Table S6), particularly LOC_Os01g50400 (6.27-fold increase after 2 h at 4 °C). Therefore, we speculated that the Ca2+ and MAPK cascade pathway may be involved in chilling tolerance in Gr89-1 seedlings.

Under cold stress, increased JA content triggers COI1-mediated degradation of JAZs, and this releases ICEs from repression (Hu et al. 2017). JA acts as an upstream signal in the ICE-CBF/DREB1 pathway, which positively regulates cold tolerance in Arabidopsis and tomato (Hu et al. 2013; Wang et al. 2016). Several TF family members, such as AP2/EREBP, NAC, bHLH, R2R3-MYB, and WRKY, are recruited in JA signaling (Goulas et al. 2006). According to our data, 35 AP2/EREBP genes were differentially regulated at low temperature, of which 25 were upregulated in seedlings (Fig. 8 and Supplementary Table S7). OsDREB1A (LOC_Os09g35030), OsDREB1B (LOC_Os09g35010), and OsDREB1C (LOC_Os06g03670) were highly upregulated in Gr89-1, consistent with previous reports that CBF/DREB1 has a central role in chilling tolerance in plants (Chinnusamy et al. 2007; Dubouzet et al. 2003). In addition, most DEGs encoding WRKY, MYB, NAC, and C2H2 TF genes were upregulated in our study, indicating that induction of the AP2/ERF, WRKY, MYB, NAC, and C2H2 families of genes was required for chilling stress tolerance of Gr89-1. Therefore, we inferred that the significant JA accumulation may play a positive role in Gr89-1 adaptation to low temperatures.

A large number of osmoprotectants are synthesized downstream of the transcriptional regulatory network (Krasensk et al. 2012). Overexpression of CBF3 increases levels of proline and total soluble sugars in non-acclimated and cold-acclimated Arabidopsis (Achard et al. 2008; Cook et al. 2004; Gilmour et al. 2004, 2000). As osmoprotectants and nutrients, soluble sugars are activated to protect the plasma membrane against damage caused by chilling stress (Tarkowski and Van den Ende 2015). In this study, the total soluble sugar contents increased sharply after 6 h of chilling stress and were maintained at a high level from 6 to 48 h compared with control seedlings (Fig. 9 and Supplementary Table S8). Overexpression of OsTPP1 and OsTPS1 increases levels of trehalose in rice, and activation of trehalose biosynthesis increases plant cold resistance (Ge et al. 2008; Habibur Rahman Pramanik and Imai 2005; Li et al. 2011; Miranda et al. 2007). Higher concentration of trehalose and significantly upregulated expression of the key enzymes involved in the biosynthesis of trehalose was detected in Gr89-1 seedlings (Fig. 9 and Supplementary Table S8). These data indicated that these soluble sugars may have major impacts on osmotic adjustment during cold stress in Gr89-1 seedlings. OsMAPK3 phosphorylates OsbHLH002/OsICE1 and inhibits its ubiquitination by OsHOS1, and OsbHLH002 activates OsTPP1 and enhances the cold tolerance of rice (Zhang et al. 2017a, b). Therefore, we speculated that the MAPK cascade pathway involved in chilling tolerance in Gr89-1 seedlings by increasing soluble sugars.

Undoubtedly, cold stress has a profound effect on cell wall polysaccharide composition and cell wall-modifying enzyme activities (Miura and Furumoto 2013). Pectin is a major polysaccharide of primary cell walls and is involved in wall plasticity and cellular adhesion. Pectin content was shown to increase in response to low temperatures in oilseed rape, leaves, pea epicotyls, and bromeliads (Carvalho et al. 2013; Kubacka-Zębalska and Kacperska 1999; Solecka et al. 2008; Weiser et al. 1990). In this study, four pectin biosynthesis-related enzymes were upregulated during cold exposure (Fig. 10 and Supplementary Table S9), particularly LOC_Os03g47530 (7.86-fold after 2 h at 4 °C). Cell wall-modifying enzymes, such as pectin methylesterase (CE8), polygalacturonase (GH28), pectin/pectate lyase-like (PL1), and pectin acetylesterase (CE13), are involved in cell wall plasticity/rheology (Sénéchal et al. 2014). All of these enzymes were differentially expressed during cold exposure (Fig. 6 and Supplementary Table S9). EXPs (Cosgrove 2005; Marowa et al. 2016), GHs (Buchanan et al. 2012; Glass et al. 2015; Wei et al. 2015), and XTHs (Nishitani and Vissenberg 2006; Rose et al. 2002) participate in the cell wall loosening process (Li et al. 2003; Minic and Jouanin 2006). EXP genes are activated in rice and Arabidopsis, while EXPs and XTHs are repressed in sweet potato (Imin et al. 2004; Noh et al. 2009; Yamauchi et al. 2004). Overexpression of AtXTH21 improves freezing tolerance in transgenic Arabidopsis plants (Shi et al. 2014). In the present study, the expression of EXP genes was downregulated, while that of XTHs and GHs (GH9, GH17, GH18, and GH28) was upregulated in seedlings (Fig. 5 and Supplementary Table S9). Cold stress also induces changes in plant cell wall proteins, such as hydroxyproline-rich glycoproteins (HRGPs). HRGPs are classified into three subfamilies: AGPs, EXTs, and PRPs (Showalter et al. 2010). Overexpression of GhAGP31 improves cold tolerance in transgenic Arabidopsis seedlings (Gong et al. 2012). In our study, nine AGP, seven EXT, and eight PRP genes were differentially expressed in seedlings exposed to cold stress, indicating that the expression of cell wall-related genes enhances the low temperature resistance in Gr89-1 seedlings.

By analyzing transcriptional profile, we found that increase in endogenous JA content and osmoprotectants and change cell wall composition in seedlings under chilling stress could enhance the cold tolerance of Gr89-1. These results may offer clues for improvement mechanisms of response to low temperature and cultivation regulation at rice seedling stage.

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Funding

The research was financially supported by the performance incentive guide special project of Chongqing Academy of Agricultural Sciences (cstc2018jxjl80026).

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Contributions

KL conceived and designed the experiments. XP and HW carried out the experiments. WZ, XJ, LG, and WB analyzed the data. XP and KL wrote and revised the paper. All authors read and approved the final manuscript.

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Correspondence to Kairong Lei.

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Key Message

• Glutinous rice 89-1 (Gr89-1) improves its response to chilling stress by increasing the production of osmoprotectants (soluble sugar, sucrose, and trehalose), modifying its cell wall structure and composition, and altering the JA biosynthetic and signal transduction pathways.

Supplementary Information

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Supplementary file1 Table S1. Sequencing saturation analysis. (DOCX 299 KB)

Supplementary file2 Table S2. Primers and corresponding sequences used for quantitative RT-PCR analysis. (XLSX 10 KB)

Supplementary file3 Table S3. Categorization and abundance of tags in Gr89-1. (DOCX 20 KB)

Supplementary file4 Table S4. Significantly-regulated DEGs at all the time points of chilling stress. (XLSX 310 KB)

Supplementary file5 Table S5. DEGs associated with phytohormone metabolism in seedlings. (XLSX 378 KB)

Supplementary file6 Table S6. DEGs associated with protein kinases in seedlings. (XLSX 363 KB)

Supplementary file7 Table S7. DEGs associated with transcription factor families in seedlings. (XLSX 394 KB)

Supplementary file8 Table S8. DEG associated with osmotic adjustment in seedlings. (XLSX 362 KB)

Supplementary file9 Table S9. DEGs associated with cell wall components in seedlings. (XLSX 370 KB)

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Pan, X., Wu, H., Hu, M. et al. Global Analysis of Gene Expression Profiles in Glutinous Rice 89-1 (Oryza sativa L.) Seedlings Exposed to Chilling Stress. Plant Mol Biol Rep (2021). https://doi.org/10.1007/s11105-020-01278-z

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Keywords

  • Glutinous rice 89-1 (spp. japonica)
  • Chilling stress
  • Transcriptional regulation
  • Sugar
  • Jasmonic acid
  • Cell wall-related genes