Abstract
NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in the process of abiotic stress response in plants. However, little is known about the functional roles of NACs in Picea (P.) wilsonii. In this study, we functionally characterized a novel P. wilsonii NAC transcription factor PwNAC30 by heterologous expression in Arabidopsis. The results showed that PwNAC30 is mainly localized in the nucleus by transient expression in Nicotiana benthamiana and can function as a nuclear localization transcription factor. β-Glucuronidase (GUS) staining in transgenic Arabidopsis (PwNAC30-promoter-GUS) confirmed the expression pattern of PwNAC30 throughout the plant development process, which is expressed in most of the plant tissues except stamens and petals. Overexpression of PwNAC30 in Arabidopsis significantly repressed the tolerance of seedlings and mature plants to both drought and salt stresses, while exerting no influence on growth and development of plants. The accumulation of reactive oxygen species (ROS) was significantly increased in transgenic plants, and the expression of some stress-responsive genes was obviously inhibited in PwNAC30 overexpression lines. Our study reveals that PwNAC30 functions as a negative regulator of plant tolerance to drought or salt stress, which provides new insights into abiotic tolerance mechanisms in woody plants.
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Acknowledgments
We thank Professor Dapeng Zhang (College of Life Sciences, Tsinghua University) for providing the pCAMBIA1205 vector and pBI121 vector.
Funding
This research was funded by a grant from the Agricultural Ministry of China, grant number 2016ZX08009-003.
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LZ and KL conceived the project and designed the experiments; LZ collected the samples; KL, YY, YM, and AW performed the experiments; KL conducted the data analysis and drafted the manuscript; LZ revised the manuscript. All authors read and approved the final manuscript.
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Key Message
The function of PwNAC30, which is identified from Picea wilsonii, is characterized by heterologous expression in Arabidopsis. Our results demonstrated that PwNAC30, as a nuclear localization transcription factor, plays a negative role in drought and salt stress response in plants; moreover, the inhibition mechanism of tolerance to abiotic stress in transgenic plants is achieved by the accumulation of reactive oxygen species (ROS) and the downregulation of stress-related genes.
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Fig. S1
Detection of PwNAC30 in transcription Arabidopsis. (a) Validation of PwNAC30 by RT-PCR. (b) The relative expression level of PwNAC30 in transgenic plants. WT, wild-type; NC, plant lines transformed with empty vectors; OE-2 and OE-4, transgenic lines of PwNAC30 overexpression. For qRT-PCR, AtActin was used as reference gene. Each value is the mean value ± SE of three independent determinations, and different letters indicate significant differences at P < 0.05 by Duncan’s multiple range test (PNG 1640 kb)
Fig. S2
Growth characteristics of PwNAC30 overexpression lines. (a, e) Plant heights of OE lines, WT and NC plants under normal growth condition. (b) Detached rosette leaves of OE lines, WT and NC plants under normal growth condition. (c) Flower organs of OE lines, WT and NC plants under normal growth condition. (d, f) Maturing siliques of OE lines, WT and NC plants. (g) Flowering times of OE lines, WT and NC plants under normal growth condition (WT, wild-type; NC, plant lines transformed with empty vectors; OE-2 and OE-4, transgenic lines of PwNAC30 overexpression). Each value is the mean value ± standard error of three independent determinations, and different letters indicate significant differences at P < 0.05 by Duncan’s multiple range test (PNG 6033 kb)
Fig. S3
Effects of exogenous ABA on the activity of the promoter. (a) GUS activity driven by the promoter of PwNAC30 under different concentrations of exogenous ABA. (b) Analysis of the PwNAC30 promoter under different ABA treatments through dual-luciferase reporter assay (PC, 35S-GUS, empty pBI121 vector transformed into N. benthamiana leaves; NC, N. benthamiana injected with buffer solution). Each value is the mean value ± standard error of three independent determinations, and different letters indicate significant differences at P < 0.05 by Duncan’s multiple range test (PNG 4144 kb)
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Liang, Kh., Wang, Ab., Yuan, Yh. et al. Picea wilsonii NAC Transcription Factor PwNAC30 Negatively Regulates Abiotic Stress Tolerance in Transgenic Arabidopsis. Plant Mol Biol Rep 38, 554–571 (2020). https://doi.org/10.1007/s11105-020-01216-z
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DOI: https://doi.org/10.1007/s11105-020-01216-z