Plant Molecular Biology Reporter

, Volume 33, Issue 5, pp 1232–1244 | Cite as

Cloning and Characterization of a GmSAGT1 Gene Whose Transcriptional Expression Is Associated with the Level of Resistance to Soybean Downy Mildew

  • Wenli Sun
  • Chao Yin
  • Mei Li
  • Huaguang Zhang
  • Shuangfeng Shi
  • Yuanhua Wu
  • Qiao Wu
  • Shirong Jia
  • Zhixing Wang
  • Yuhui Liu
Original Paper


We have cloned a GmSAGT1 gene coding for a bi-functional enzyme of serine/alanine glyoxylate aminotransferase from a soybean variety Zaofeng5 resistant to soybean downy mildew (SDM). The GmSAGT1 expressed in Escherichia coli exhibited serine glyoxylate aminotransferase (SGT) and alanine glyoxylate aminotransferase (AGT) activity that produced 20-fold more glycine than that of the control. The amount of hydrogen peroxide (H2O2) dramatically increased in E. coli expressing GmSAGT1, and bacterial growth was severely inhibited. Real-time polymerase chain reaction (RT-PCR) showed that GmSAGT1 could be detected in Zaofeng5 prior to salicylic acid (SA) treatment and increased by fivefold after SA induction. In contrast, it was not detectable in susceptible variety Heinong10 prior to and only slightly increased after SA induction. Fifteen soybean varieties belonging to highly resistant (HR), medium resistant (MR), resistant (R), susceptible (S), and highly susceptible (HS) category were used for real-time quantitative RT-PCR (qRT-PCR) analysis, which showed the average expression level of GmSAGT1 in HR group was 13 times of that in HS group. Northern blot analysis indicated that the expression level of GmSAGT1 in HS, S, MR, and R group was 5, 22, 39, and 65 % of that in HR, respectively. Transgenic tobacco expressing GmSAGT1 increased resistance to a fungal pathogen Rhizoctonia solani. Our results have demonstrated that higher expression of GmSAGT1 gene is closely correlated to increased SDM resistance.


Soybean Downy mildew Salicylic acid Serine/alanine glyoxylate aminotransferase Hydrogen peroxide (H2O2



Alanine glyoxylate aminotransferase


Disease index


Expressed sequence tag


Glycolate oxidase


Hydrogen peroxide


Highly resistant


Highly susceptible


Isopropyl β-d-thiogalactoside


Melon downy mildew


Medium resistant


Murashige and Skoog medium

PR proteins

Pathogenic-related proteins

R gene

Resistance gene




Reactive oxygen species




Salicylic acid


Systemic acquired resistance


Soybean downy mildew


Serine glyoxylate aminotransferase



This work was financially supported by the National High-tech R&D Program (2011AA100606) and “The development of new varieties of animals and crops” under the National High Priority R&D Program (2013ZX08009-003-005). We sincerely thank Prof. Lijuan Qiu in the National Germplasm Bank, China for kindly providing us seeds of soybean varieties.

Supplementary material

11105_2014_818_MOESM1_ESM.doc (76 kb)
ESM 1 (DOC 76 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Wenli Sun
    • 1
    • 2
  • Chao Yin
    • 1
  • Mei Li
    • 1
  • Huaguang Zhang
    • 1
  • Shuangfeng Shi
    • 1
  • Yuanhua Wu
    • 2
  • Qiao Wu
    • 2
  • Shirong Jia
    • 1
  • Zhixing Wang
    • 1
  • Yuhui Liu
    • 1
  1. 1.Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijingChina
  2. 2.College of Plant ProtectionShenyang Agricultural UniversityShenyangChina

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