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Plant Molecular Biology Reporter

, Volume 33, Issue 3, pp 716–730 | Cite as

Proteomic Analysis of PEG-Induced Drought Stress Responsive Protein in TERF1 Overexpressed Sugarcane (Saccharum officinarum) Leaves

  • M. Anisur Rahman
  • Lei Ren
  • Wei Wu
  • Yanchun Yan
Original Paper

Abstract

Drought is the major abiotic stress limiting sugarcane growth and productivity. ERF proteins regulate a variety of stress responses in plant. Overexpression of TERF1 can enhance the tolerance of transgenic sugarcane to drought stress. To improve the efficiency of sugarcane breeding, better understanding of the tolerance mechanism at molecular level is required. Two-dimensional gel electrophoresis (2-DE) coupled tandem mass spectrometry (MS/MS) analyses were conducted to compare the leaf proteome of the TERF1 OE and WT sugarcane plants to PEG stress. Using statistical program, 50 significantly differential protein spots were detected, of which 36 spots were identified by PMF and MS/MS fragmentation. Most of the identified proteins corresponded to metabolism, energy, protein synthesis, and disease/defense. Results implicated that the involvement of different metabolic pathways that may be activated in the TERF1 overexpressed transgenic sugarcane to cope with drought environment. Of the identified proteins, abundance of pentatricopeptide repeat (PPR) containing protein and peptidyl prolyl cis-trans isomerase (PPIase) were decreased, but the abundance of vital proteins, such as metabolism protein (14-3-3 like protein), photosynthetic protein (RuBisCO large subunit, PEP carboxylase), ferredoxin, glyceraldehyde 3-phosphate dehydrogenase, elongation factor Tu, several small heat shock proteins, and peroxidases were increased. Analysis of protein properties showed that majority of the differentially abundant proteins associated with drought were stable, hydrophilic, and transmembrane proteins. Thus, the results of our study unravel the regulatory mechanism of TERF1 for drought stress tolerance of transgenic sugarcane and provide new insight into adaptation to osmotic stress through altering the expression of particular proteins.

Keywords

Proteomics PEG stress TERF1 overexpression Sugarcane 2-DE Mass spectrometry 

Abbreviations

TERF1

Tomato ethylene responsive factor 1

TCA

Trichloroacetic acid

2-DE

Two-dimensional gel electrophoresis

CHAPS

3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate

OE

Overexpressed

MOWSE

Molecular weight search

WT

Wild-type

IEF

Isoelectric focusing

PEG

Polyethylene glycol

SDS

Sodium dodecyl sulfate

ERF

Ethylene responsive factor

PMF

Peptide mass fingerprinting

RWC

Relative water content

MS

Mass spectrometry

DTT

Dithiothreitol

NCBI

National center for biotechnology information

MALDI-TOF-TOF-MS

Matrix-assisted laser desorption/ionization-time of flight/time of flight tandem mass spectrometry

ROS

Reactive oxygen species

Notes

Acknowledgments

This research was supported by the National Agricultural Technology Project (NATP), PIU-BARC, Phase-1, IDA Credit 4386, Bangladesh. We thank the Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China for providing plant materials.

Supplementary material

11105_2014_784_MOESM1_ESM.docx (326 kb)
ESM 1 (DOCX 325 kb)
11105_2014_784_MOESM2_ESM.docx (20 kb)
ESM 2 (DOCX 20 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Graduate SchoolChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Bangladesh Sugarcane Research InstitutePabnaBangladesh

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