A Novel U-Box Protein Gene from “Zuoshanyi” Grapevine (Vitis amurensis Rupr. cv.) Involved in Cold Responsive Gene Expression in Arabidopsis thanliana
- 327 Downloads
Ubiquitination plays an important role in posttranscriptional modification of proteins. As a component of the ubiquitin conjugation system, E3 ligase is believed to be a key element determining the substrate specificity of ubiquitination. In this research, a novel putative U-box type E3 ligase VaPUB (plant U-box protein) was isolated from a very cold-hardy “Zuoshanyi” (Vitis amurensis Rupr. cv.) grapevine. Real-time PCR demonstrated that PUB was upregulated by cold stress and expressed more strongly in cold-tolerant grapevines than in cold-sensitive grapevines. In cold-hardy V. amurensis Zuoshanyi grapevine, the expression of PUB was upregulated by cold in all tissues, although more dramatic increases were found in the leaves and shoot tips than in the roots or stems. Over-expression of VaPUB in Arabidopsis resulted in an enhancement of cold and salt stress tolerance. The transcription factors, CBF1/DREB1B and CBF3/DREB1A, and cold inducing genes, COR15A, COR47, KIN1, RD29A, and SuSy, were expressed in a higher level in VaPUB transgenic plants than the wild type. In contrast, the expression of transcription factor CBF2/DREB1C was repressed by VaPUB. These results demonstrated that VaPUB enhanced plant cold and salt stress tolerance and its over-expression affected the expression of cold responsive genes.
KeywordsVitis amurensis U-box protein Abiotic stress Transgenic Arabidopsis
This work was supported by the Chinese Universities Scientific Fund (Grant No. 2012RC019) and earmarked fund for Chinese Agricultural Research System (CARS-30-yz-2).
- Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S et al (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA-binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression respectively in Arabidopsis. Plant Cell 10:1391–1406CrossRefPubMedCentralPubMedGoogle Scholar
- Mano Y, Nakazumi H, Takeda K (1996) Varietal variation in and effects of some major genes on salt tolerance at the germination stage in barley. Breeding Sci 46:227–233Google Scholar
- Stockinger EJ, Gilmour SJ, Thomashow MF (1997) Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A 94:1035–1040CrossRefPubMedCentralPubMedGoogle Scholar
- Wu J, Zhang Y, Wu N, Zhang H, Jiao L et al (2012) Identification and expression analysis of cold-regulated genes in Vitis amurensis Rupr. cv. Zuoshan-1. J Hortic Sci Biotech 87:557–564Google Scholar