Plant Molecular Biology Reporter

, Volume 33, Issue 3, pp 557–568 | Cite as

A Novel U-Box Protein Gene from “Zuoshanyi” Grapevine (Vitis amurensis Rupr. cv.) Involved in Cold Responsive Gene Expression in Arabidopsis thanliana

  • Li Jiao
  • Yali Zhang
  • Jiao Wu
  • Huiqin Zhang
  • Jiang Lu
Original Paper


Ubiquitination plays an important role in posttranscriptional modification of proteins. As a component of the ubiquitin conjugation system, E3 ligase is believed to be a key element determining the substrate specificity of ubiquitination. In this research, a novel putative U-box type E3 ligase VaPUB (plant U-box protein) was isolated from a very cold-hardy “Zuoshanyi” (Vitis amurensis Rupr. cv.) grapevine. Real-time PCR demonstrated that PUB was upregulated by cold stress and expressed more strongly in cold-tolerant grapevines than in cold-sensitive grapevines. In cold-hardy V. amurensis Zuoshanyi grapevine, the expression of PUB was upregulated by cold in all tissues, although more dramatic increases were found in the leaves and shoot tips than in the roots or stems. Over-expression of VaPUB in Arabidopsis resulted in an enhancement of cold and salt stress tolerance. The transcription factors, CBF1/DREB1B and CBF3/DREB1A, and cold inducing genes, COR15A, COR47, KIN1, RD29A, and SuSy, were expressed in a higher level in VaPUB transgenic plants than the wild type. In contrast, the expression of transcription factor CBF2/DREB1C was repressed by VaPUB. These results demonstrated that VaPUB enhanced plant cold and salt stress tolerance and its over-expression affected the expression of cold responsive genes.


Vitis amurensis U-box protein Abiotic stress Transgenic Arabidopsis 



This work was supported by the Chinese Universities Scientific Fund (Grant No. 2012RC019) and earmarked fund for Chinese Agricultural Research System (CARS-30-yz-2).

Supplementary material

11105_2014_783_Fig8_ESM.gif (61 kb)
Supplementary Fig. 1

Sequence alignment of PUB between V. amurensis ‘Zuoshanyi’ and V. quinquangularis ‘Maoputao’. The U-box domain is indicated by a solid line. Sequences conserved in these two cultivars are shown in black (GIF 60.8 kb)

11105_2014_783_MOESM1_ESM.tif (812 kb)
High Resolution Image (TIFF 812 kb)
11105_2014_783_MOESM2_ESM.docx (14 kb)
Supplementary Table 1 (DOCX 14.2 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Li Jiao
    • 1
  • Yali Zhang
    • 1
  • Jiao Wu
    • 1
  • Huiqin Zhang
    • 1
  • Jiang Lu
    • 1
  1. 1.The Viticulture and Enology Program, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina

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