Plant Molecular Biology Reporter

, Volume 33, Issue 1, pp 22–42 | Cite as

A Gene Encoding Cold-Circadian Rhythm-RNA Binding-Like Protein (CCR-Like) from Upland Cotton (Gossypium hirsutum L.) Confers Tolerance to Abiotic Stresses in Transgenic Tobacco

  • Gurusamy Dhandapani
  • Mogilicherla Kanakachari
  • Kethireddy Venkata Padmalatha
  • Mullapudi Lakshmi Venkata Phanindra
  • Vivek Kumar Singh
  • Sanagala Raghavendrarao
  • Narayanasamy Jayabalan
  • Azhagiyamanavalan Lakshmi Prabha
  • Polumetla Ananda Kumar
Original Paper


Water-deficit is the major abiotic stress factor that limits the yield and quality of cotton produced around the world. We observed earlier that a CCL (CCR-like; cold-circadian rhythm-RNA binding like) gene has been differentially expressed during boll development in cotton under drought stress in the field. Isolation and functional characterization of GhCCL from upland cotton (Gossypium hirsutum L. cv. Bikaneri Narma) was carried out in the present study. We studied the GhCCL gene structure and organization and demonstrated for the first time that GhCCL may be involved in abiotic stress tolerance response in plants. RT-PCR analysis indicated that GhCCL is differentially regulated in cotton seedlings by abiotic stresses such as salt, mannitol, cold, heat, dehydration, wounding and jasmonic acid, salicylic acid, and hydrogen peroxide. In silico and subcellular localization analysis suggested that GhCCL is localized in the chloroplasts. Constitutive expression of GhCCL in tobacco (Nicotiana tabacum var. Petit Havana) conferred tolerance to water-deficit stress and salt stresses during seed germination on amended MS media. The transgenic plants showed better growth performance and increased fresh weight under long-term stress. The transgenic plants grown in the glass house tolerated water-deficit stress (by withholding water) and recovered upon rewatering (45 days) whereas WT plants did not survive. Characterization of the GhCCL promoter sequence by in silico analysis showed various light, stress, tissue specific, and hormone responsive cis-elements. The present study suggested that GhCCL positively regulates the response to abiotic stresses, especially water-deficit stress in transgenic plants and that the overexpression of GhCCL may enhance the stability of mRNA thereby conferring tolerance to abiotic stresses.


Cold-circadian rhythm-RNA binding Gossypium hirsutum Water-deficit stress Transgenic tobacco 



This work was supported by funds from the Indian Council of Agricultural Research (ICAR) under the National Agricultural Innovation Project (NAIP/C4/C10103). Funds from the Department of Biotechnology (DBT), Government of India are gratefully acknowledged. We thank Dr. I.S Katageri, University of Agricultural Sciences, Dharwad for providing the drought-induced cotton boll samples used in the study and Dr. Ajay Jain, National Research Centre on Plant Biotechnology, New Delhi for providing the binary vector pCAMBIA1302. We also thank Dr. Shelly Praveen and Mr. Vipin Kumar, Division of Plant Pathology, Indian Agricultural Research Institute (IARI), New Delhi for providing confocal microscope facility and technical help for subcellular localization study.

Supplementary material

11105_2014_729_MOESM1_ESM.docx (22 kb)
ESM 1 (DOCX 21.9 kb)
11105_2014_729_MOESM2_ESM.pptx (54 kb)
Fig. S1 T-DNA regions of binary vectors used for tobacco transformation. a Cotton GhCCL gene was cloned in pBI121 binary vector in the place of GUS reporter gene driven by CaMV35S promoter. b pBI121 binary vector carrying cotton GhCCL gene driven by Arabidopsis thaliana rd29A promoter. The right border (RB) junction fragment of the T-DNA is >3.3, 3.4-kb in 35S::GhCCL and Atrd29A::GhCCL, respectively (the distance between BamHI and RB). (PPTX 54 kb)
11105_2014_729_MOESM3_ESM.pptx (38 kb)
Fig. S2 Predicted conserved domain in GhCCL protein by NCBI-Conserver domain database (CDD). (PPTX 38 kb)
11105_2014_729_MOESM4_ESM.pptx (212 kb)
Fig. S3 PCR analysis of transgenic tobacco plants. T 0 transgenic tobacco analysed with NPTII and GhCCL gene specific primer (a, b), respectively. c PCR analysis of selected T 1 transgenic tobacco plants with GhCCL gene specific primer. W-water control C-WT control, A-35S::GhCCL, B-rd29A::GhCCL, AP-35S::GhCCL binary plasmid, BP-rd29A::GhCCL and M1, M2:1-kb and 100-bp ladder, respectively. (PPTX 212 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Gurusamy Dhandapani
    • 1
    • 2
  • Mogilicherla Kanakachari
    • 1
    • 2
  • Kethireddy Venkata Padmalatha
    • 1
  • Mullapudi Lakshmi Venkata Phanindra
    • 1
  • Vivek Kumar Singh
    • 1
  • Sanagala Raghavendrarao
    • 1
  • Narayanasamy Jayabalan
    • 2
  • Azhagiyamanavalan Lakshmi Prabha
    • 2
  • Polumetla Ananda Kumar
    • 1
    • 3
  1. 1.National Research Centre on Plant BiotechnologyNew DelhiIndia
  2. 2.Department of Plant ScienceBharathidasan UniversityTiruchirappalliIndia
  3. 3.Institute of BiotechnologyAcharya NG Ranga Agricultural UniversityHyderabadIndia

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