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Plant Molecular Biology Reporter

, Volume 31, Issue 5, pp 1068–1076 | Cite as

Characterization and Expression Analysis of a Glutathione Reductase Gene from Antarctic Moss Pohlia nutans

  • Pengying Zhang
  • Shenghao Liu
  • Kaoshan Chen
Original Paper

Abstract

Glutathione reductase (GR) is a flavoprotein oxidoreductase and plays an important role in response to oxidative stresses in plants. A cDNA-encoding cytosolic GR [GenBank accession number GACA01029426, designated as Pohlia nutans glutathione reductase gene (PnGR)] was successfully cloned from Antarctic moss P. nutans. The full-length PnGR cDNA has 1,654 bp nucleotides with an open reading frame of 1,494 bp, encoding 497 amino acid residues. The deduced amino acid sequence of PnGR had 87.0 % identity with GR in Physcomitrella patens subsp. patens. The phylogenetic analysis showed that PnGR is clustered together with known cytosolic GR in other plants. In addition, the subcellular localization analysis by observing the transient expression of PnGR–green fluorescent protein fusion protein in Arabidopsis thaliana mesophyll protoplasts also revealed PnGR targeting to cytosol in plant cells. The expression patterns of PnGR under different abiotic stresses were determined by real-time PCR. Compared to the normal condition, the maximal mRNA accumulation of PnGR increased 3.82-fold at 4 °C, 2.92-fold at 10 °C, 4.14-fold with 200 mM NaCl, and 3.17-fold with drought stress, respectively. Together, our results suggested that the inducible PnGR might play an important role in Antarctic moss P. nutans acclimatizing to polar environment.

Keywords

Cold adaptation Pohlia nutans Glutathione reductase Stress 

Notes

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (40906103 and 41206176) and Basic Scientific Research Foundation of the First Institute of Oceanography (SOA).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pengying Zhang
    • 1
    • 2
  • Shenghao Liu
    • 1
    • 2
    • 3
  • Kaoshan Chen
    • 1
    • 2
  1. 1.National Glycoengineering Research Center and College of Life ScienceShandong UniversityJinanPeople’s Republic of China
  2. 2.The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of EducationShandong UniversityJinanPeople’s Republic of China
  3. 3.Key Laboratory of Marine Bioactive Substance, The First Institute of OceanographyState Oceanic AdministrationQingdaoPeople’s Republic of China

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