Identification and Characterisation of Cotton Boll Wall-Specific Gene Promoters for Future Transgenic Cotton Varieties
- 449 Downloads
Current transgenic cotton varieties constitutively express transgenes encoding anti-pest proteins to protect against plant damage caused by insect attack. However, restricting the spatial expression of transgenes to the tissues in which their products are required is likely to improve crop performance and reduce environmental impacts. Therefore, we sought to identify native gene promoters that would restrict transgene expression to the boll wall of the cotton plant. Six abundant mRNAs that accumulated preferentially in the boll wall were identified, and the gene promoters of two of these mRNAs were identified, isolated and characterised. The promoters of a proline-rich protein gene (GhPRP3) and a chalcone synthase gene (GhCHS1) were demonstrated to drive boll wall-preferential expression of a reporter gene in a transient transformation system. In silico analyses of the GhPRP3 and GhCHS1 promoters identified numerous previously identified cis-acting regulatory elements (CAREs) as well as the presence of three novel shared CAREs. The identification and characterisation of these promoters provides an important step in the development of the next generation of transgenic plants.
KeywordsBoll wall Promoter Cotton Gossypium hirsutum Chalcone synthase Proline-rich protein
This work was partially funded by the Cotton Research and Development Corporation (Narrabri, NSW, Australia) as projects UA11C (DJL) and UA7C (SJO, JNT).
- Bailey TL, Elkan C (1994) Fitting a mixture model by expectation maximization to discover motifs in biopolymers. In: Proceedings International Conference on Intelligent Systems for Molecular Biology, 1994. AAAI Press, Menlo Park, California, pp 28–36Google Scholar
- Fernandez AI, Viron N, Alhagdow M, Karimi M, Jones M, Amsellem Z, Sicard A, Czerednik A, Angenent G, Grierson D, May S, Seymour G, Eshed Y, Lemaire-Chamley M, Rothan C, Hilson P (2009) Flexible tools for gene expression and silencing in tomato. Plant Physiol 151(4):1729–1740PubMedCrossRefGoogle Scholar
- James C (2007) Global status of commercialized biotech/GM crops: 2007. ISAAA brief No. 37. ISAAA, IthacaGoogle Scholar
- Mang HG, Lee JH, Park JA, Pyee J, Pai HS, Kim WT (2004) The CaPRP1 gene encoding a putative proline-rich glycoprotein is highly expressed in rapidly elongating early roots and leaves in hot pepper (Capsicum annuum L. cv. Pukang). Biochimica Et Biophysica Acta 1674(1):103–108PubMedCrossRefGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Wilkins TA, Jernstedt JA (1999) Molecular genetics of developing cotton fibers. In: Basra AM (ed) Cotton fibers. Hawthorne Press, New York, pp 231–267Google Scholar