Characterization of a Novel Pollen-Specific Promoter from Wheat (Triticum Aestivum L.)
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PSG076 is a pollen-specific gene isolated from wheat. The 1.4-kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG076 promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco. Histochemical analysis in transgenic tobacco showed that GUS activity was detected in late bicellular pollen grains and increased rapidly in mature pollen. GUS activity was also detected in pollen tubes of transgenic tobacco. No GUS activity was found in other floral and vegetable tissues. These results indicate that the PSG076 promoter directs pollen-specific activity at late stages of pollen development and pollen tube growth. Deletion analysis showed that a 0.4 kb fragment of the promoter was enough to confer pollen-specific expression.
KeywordsWheat Pollen-specific promoter PSG076 gene Tobacco Genetic transformation
This work was supported by the China Postdoctoral Science Foundation (No. 20100471125), the Genetically Modified New Varieties of Major Projects of China (2011ZX08002-004, 2011ZX08010-004, 2009ZX08016-001A, 2009ZX08002-001B, 2009ZX08002-014B, 2009ZX08002-013B), Key Projects of Science and Technology Research of Chinese Ministry of Education (Grant No. 109105) and Key Projects of International Cooperation of Chinese Ministry of Science and Technology (2009DFB30340).
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