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Plant Molecular Biology Reporter

, Volume 30, Issue 5, pp 1246–1252 | Cite as

Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum)

  • Mehmet Cengiz Baloglu
  • Mehmet Tufan Oz
  • Huseyin Avni Oktem
  • Meral Yucel
Original Paper

Abstract

NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. Compared to control samples, a quick response at the transcription level of TaNAC69-1 was observed after 3 h of salt treatment with a ninefold upregulation. Highest level of expression was observed at 24 and 48 h posttreatment under heat and salinity treatments, respectively. Meanwhile, expression of TtNAMB-2 was significantly induced by salt and low-temperature stresses. Salt treatment induced expression of TtNAMB-2 and caused a 13-fold increase in transcript copy numbers at 48 h posttreatment. Examination of expression changes under abiotic stresses may provide important information for understanding roles of TaNAC69-1 and TtNAMB-2 genes which might be involved in response to environmental stresses.

Keywords

NAC-type transcription factors Wheat Gene expression RT-qPCR 

Notes

Acknowledgments

We would like to thank METU Central Laboratory, Molecular Biology and Biotechnology R&D Center for analysis of RNA samples. This study was supported by the Research Fund of METU grant no. BAP-08-11-DPT2002K120510 and TÜBİTAK grant no. 108O786.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Mehmet Cengiz Baloglu
    • 1
    • 2
  • Mehmet Tufan Oz
    • 1
  • Huseyin Avni Oktem
    • 1
  • Meral Yucel
    • 1
  1. 1.Department of Biological SciencesMiddle East Technical UniversityAnkaraTurkey
  2. 2.Department of BiologyKastamonu UniversityKastamonuTurkey

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