Expression Analysis of TaNAC69-1 and TtNAMB-2, Wheat NAC Family Transcription Factor Genes Under Abiotic Stress Conditions in Durum Wheat (Triticum turgidum)
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NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. In the current study, expression profiles of wheat NAC-type transcription factor genes, TaNAC69-1 and TtNAMB-2, were examined under drought, salt, cold, and heat stress conditions in wheat. Based on reverse transcription quantitative PCR results, TaNAC69-1 was strongly expressed under drought, salinity, and high-temperature stress conditions. Compared to control samples, a quick response at the transcription level of TaNAC69-1 was observed after 3 h of salt treatment with a ninefold upregulation. Highest level of expression was observed at 24 and 48 h posttreatment under heat and salinity treatments, respectively. Meanwhile, expression of TtNAMB-2 was significantly induced by salt and low-temperature stresses. Salt treatment induced expression of TtNAMB-2 and caused a 13-fold increase in transcript copy numbers at 48 h posttreatment. Examination of expression changes under abiotic stresses may provide important information for understanding roles of TaNAC69-1 and TtNAMB-2 genes which might be involved in response to environmental stresses.
KeywordsNAC-type transcription factors Wheat Gene expression RT-qPCR
We would like to thank METU Central Laboratory, Molecular Biology and Biotechnology R&D Center for analysis of RNA samples. This study was supported by the Research Fund of METU grant no. BAP-08-11-DPT2002K120510 and TÜBİTAK grant no. 108O786.
- Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin J-F, Wu S-H, Swidzinski J, Ishizaki K, Leaver CJ (2005) Comparative transcriptome analysis reveals significant differences in gene expression and signaling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J 42:567–585PubMedCrossRefGoogle Scholar
- Gong W, Shen Y-P, Ma L-G, Pan Y, Du Y-L, Wang D-H, Yang J-Y, Hu L-D, Liu X-F, Dong C-X, Ma L, Chen Y-H, Yang X-Y, Gao Y, Zhu D, Tan X, Mu J-Y, Zhang D-B, Liu Y-L, Dinesh-Kumar SP, Li Y, Wang X-P, Gu H-Y, Qu L-J, Bai S-N, Lu Y-T, Li J-Y, Zhao J-D, Zuo J, Huang H, Deng XW, Zhu Y-X (2004) Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes. Plant Physiol 135:773–782PubMedCrossRefGoogle Scholar
- Gupta PK, Mir RR, Mohan A, Kumar J (2008) Wheat genomics: present status and future prospects. Int J Plant Genom. doi: 10.1155/2008/896451
- Han X, He G, Zhao S, Guo C, Lu M (2011) Expression analysis of two NAC transcription factors PtNAC068 and PtNAC154 from poplar. Plant Mol Biol Rep. doi: 10.1007/s11105-011-0350-1
- Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Cal Agric Exp Sta Ciru 347:1–32Google Scholar
- Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Osato N, Kawai J, Carninci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S (2003) Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res 10:239–247PubMedCrossRefGoogle Scholar
- Patnaik D (2001) Wheat biotechnology: a minireview. Electron J Biotech 4:1–29Google Scholar
- Rosegrant M, Paisner M, Meijer S, Witcover J (2001) Global food projections to 2020: emerging trends and alternative futures. Int Food Policy Res InstitGoogle Scholar
- Tran L-SP, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498PubMedCrossRefGoogle Scholar
- Xia N, Zhang G, Liu X-Y, Deng L, Cai G-L, Zhang Y, Xiao-Jie W, Zhao J, Huang L-L, Kang Z-S (2010a) Characterization of a novel wheat NAC transcription factor gene involved in defense response against stripe rust pathogen infection and abiotic stresses. Mol Biol Rep 37:3703–3712PubMedCrossRefGoogle Scholar