Plant Molecular Biology Reporter

, Volume 30, Issue 4, pp 949–956 | Cite as

Analysis of Differential Proteins Induced by Forchlorfenuron in Wheat

  • Na Yin
  • Xin Ma
  • Weidong Zhang
  • Deshun Feng
  • Honggang Wang
  • Lingrang Kong
  • Jichun Tian
Original Paper


To identify differential proteins induced by N-(2-chloro-4-pyridy)-N′-phenylurea or forchlorfenuron (CPPU), two-dimensional electrophoresis was used to study the variations in protein expression of wheat seedlings induced with CPPU. The expressed protein spots were identified by matrix-assisted laser desorption/ionization–time of flight mass spectrometry and protein data bank query. About 800 spots were reproducibly detected in three replicates of each sample. A total of 10 differential proteins were identified, including tRNA delta (2)-isopentenylpyrophosphate transferase (S1), probable peptide ABC transporter ATP-binding protein y4tS (S2), ribosomal RNA large subunit methyltransferase N (S3), microtubule-associated protein 6 (S4), actin-66 (Fragment) (S5), mitochondrial ATP synthase subunit alpha (S6), S-adenosylmethionine synthase 1 (S7), flavin-containing monooxygenase YUCCA4 (S8), GTPase obg (S9), and ribosomal RNA small subunit methyltransferase G (S10). These proteins were closely related to phytohormone synthesis, plant stress response, energy metabolism, protein synthesis, cytoskeleton, and transport. All of these processes may play important roles in wheat seedling development. These findings could provide valuable information for the further study of the functions and underlying mechanism of the action of CPPU. Thus, proteomic analysis revealed that CPPU treatment induces variations in many proteins involved in plant growth and development.


N-(2-chloro-4-pyridy)-N′-phenylurea (CPPU) Wheat seedlings Differential protein Mass spectrum 



Two-dimensional electrophoresis




Complementary DNA


N-(2-chloro-4-pyridy)-N′-phenylurea (forchlorfenuron)




Isoelectric focusing


Isopentyl transferase


Matrix-assisted laser desorption/ionization


Mass spectrometry


Polyacrylamide gel electrophoresis


Sodium dodecyl sulfate







We wish to thank two anonymous reviewers for helpful comments that improved the manuscript. This project was supported by the National Natural Science Foundation of China (Project no. 31171552) and the Scientific Research Award Foundation for Outstanding Young and Middle-Aged Scientists of Shandong Province of China (Project no. BS2009NY037).


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Na Yin
    • 1
  • Xin Ma
    • 1
  • Weidong Zhang
    • 1
  • Deshun Feng
    • 1
  • Honggang Wang
    • 1
  • Lingrang Kong
    • 1
  • Jichun Tian
    • 1
  1. 1.State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of AgronomyShandong Agricultural UniversityTai’anChina

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