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Plant Molecular Biology Reporter

, Volume 30, Issue 3, pp 590–598 | Cite as

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

  • Huifeng Wu
  • Xiaoli Liu
  • Liping You
  • Linbao Zhang
  • Junbao Yu
  • Di Zhou
  • Jianmin Zhao
  • Jianghua Feng
Article

Abstract

Suaeda salsa is a native halophyte in saline soils. Salinity is the most important environmental constraint for plant productivity in the Yellow River Delta. In this work, we investigated the salt-induced effects in root of S. salsa exposed to two environmentally relevant salinities for 1 week and 1 month using nuclear magnetic resonance-based metabolomics. Our results indicated that salt stress inhibited the growth of S. salsa and induced significant metabolic responses including decreased amino acids, lactate, 4-aminobutyrate, malate, choline, phosphocholine, and increased betaine, sucrose, and allantoin in root tissues of S. salsa. In addition, salinity exposures upregulated the activities of superoxide dismutase, glutathione S-transferases, peroxidase, catalase, and glutathione peroxidase in the aboveground part of seedlings of S. salsa after exposures. Overall, these results demonstrated the osmotic and oxidative stresses, disturbances in protein biosynthesis/degradation, and energy metabolism in S. salsa exposed to salinities.

Keywords

Suaeda salsa Salinity Metabolic response NMR Metabolomics Antioxidant enzyme activity 

Abbreviations

ANOVA

Analysis of variance

BADH

Betaine aldehyde dehydrogenase

CAT

Catalase

CMO

Choline monooxygenase

FDR

False discovery rate

GPx

Glutathione peroxidase

GST

Glutathione S-transferases

NMR

Nuclear magnetic resonance

PC

Principal component

PCA

Principal components analysis

POD

Peroxidase

PR

Pattern recognition

ROS

Reactive oxygen species

SAM

Significance analysis of microarray

SOD

Superoxide dismutase

TCA

Tricarboxylic acid

TSP

Sodium 3-trimethlysilyl [2,2,3,3-D4] propionate

Notes

Acknowledgements

We thank Dr. Mark Viant (School of Bioscience, The University of Birmingham) for use of the software ProMetab. This research was supported by the Project of National Science & Technology Pillar Program in “12th Five Year” Period (2011BAC02B01), The 100 Talents Program of the Chinese Academy of Sciences, Innovation Programs of the Chinese Academy of Sciences (KZCX2-YW-223 and KZCX2-YW-225) and Technology Development Program Projects of Shandong Province (2008GG20005006 and 2008GG3NS0700), and in part by the CAS/SAFEA International Partnership Program for Creative Research Teams.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Huifeng Wu
    • 1
  • Xiaoli Liu
    • 1
    • 2
  • Liping You
    • 1
    • 2
  • Linbao Zhang
    • 1
    • 2
  • Junbao Yu
    • 1
  • Di Zhou
    • 1
    • 2
  • Jianmin Zhao
    • 1
  • Jianghua Feng
    • 3
  1. 1.Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiPeople’s Republic of China
  2. 2.The Graduate School of Chinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Department of Electronic Science, Fujian Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid SurfacesXiamen UniversityXiamenPeople’s Republic of China

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