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Characterization and Functional Validation of Tobacco PLC Delta for Abiotic Stress Tolerance

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Abstract

The role of plant phospholipase C-mediated signaling has been implicated in various phases of plant growth and development. In this study, we report on the isolation and characterization of phospholipase C from tobacco and demonstrate that transcripts of phospholipase C are up-regulated in responses to drought and salt stress. These responses are likely by abscisic acid (ABA). Transgenic tobacco plants overexpressing the phospholipase C protein were found to tolerate higher levels of drought and also salinity stress. This tolerance could be mediated by the regulation of genes downstream to phospholipase mediated signaling. As a demonstration, when tested the transgenic plants showed higher transcript of heat shock factor NtHSF2, heat shock protein HSP70-3 and an AP2 domain transcription factor. Also the transgenic plants showed higher accumulation of sodium in older leaves compared to the young leaves. The present report is the first to demonstrate the role of phospholipase C in salinity stress tolerance.

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Acknowledgements

This work was supported by Department of Biotechnology, Govt. of India (Indo-Belarus) project. For providing the confocal microscope facility (Welcome Grant) and technical help, we are thankful to Dr. Shahid Jameel and Ms. Charu, respectively. We also thank Drs. V. Rajamani, J.K. Tripathi (Jawaharlal Nehru University, New Delhi) for help with ionic measurements.

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  1. Sudhir K. Sopory

    Present address: Jawaharlal Nehru University, New Delhi, 110067, India

Authors and Affiliations

  1. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Manas Kumar Tripathy, Wricha Tyagi, Mamta Goswami, Tanushri Kaul, Sneh Lata Singla-Pareek, Malireddy K. Reddy & Sudhir K. Sopory

  2. Department of Botany, University of Delhi, Delhi, 110007, India

    Manas Kumar Tripathy & Renu Deswal

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  1. Manas Kumar Tripathy
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  2. Wricha Tyagi
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Correspondence to Sudhir K. Sopory.

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Fig. S1

Representative pictures to show relative salinity and mannitol tolerance of WT and kanamycin-positive T1- NtPLCδ1 tobacco transgenic plants. Seedling growth comparison of WT and T1 transgenic lines (S10 and S11) of 40-day-old seedlings on a control, b 100 mM NaCl, c 200 mM NaCl, d 250 mM NaCl and e 200 mM mannitol, f 400 mM mannitol. (JPEG 68 kb)

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Tripathy, M.K., Tyagi, W., Goswami, M. et al. Characterization and Functional Validation of Tobacco PLC Delta for Abiotic Stress Tolerance. Plant Mol Biol Rep 30, 488–497 (2012). https://doi.org/10.1007/s11105-011-0360-z

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