Characterization and Functional Validation of Tobacco PLC Delta for Abiotic Stress Tolerance
The role of plant phospholipase C-mediated signaling has been implicated in various phases of plant growth and development. In this study, we report on the isolation and characterization of phospholipase C from tobacco and demonstrate that transcripts of phospholipase C are up-regulated in responses to drought and salt stress. These responses are likely by abscisic acid (ABA). Transgenic tobacco plants overexpressing the phospholipase C protein were found to tolerate higher levels of drought and also salinity stress. This tolerance could be mediated by the regulation of genes downstream to phospholipase mediated signaling. As a demonstration, when tested the transgenic plants showed higher transcript of heat shock factor NtHSF2, heat shock protein HSP70-3 and an AP2 domain transcription factor. Also the transgenic plants showed higher accumulation of sodium in older leaves compared to the young leaves. The present report is the first to demonstrate the role of phospholipase C in salinity stress tolerance.
KeywordsSalinity Drought Phospholipase C Tobacco Signaling Abscisic acid
This work was supported by Department of Biotechnology, Govt. of India (Indo-Belarus) project. For providing the confocal microscope facility (Welcome Grant) and technical help, we are thankful to Dr. Shahid Jameel and Ms. Charu, respectively. We also thank Drs. V. Rajamani, J.K. Tripathi (Jawaharlal Nehru University, New Delhi) for help with ionic measurements.
- Kim YJ, Kim JE, Lee JH, Lee MH, Jung HW, Bahk YY, Hwang BK, Hwang I, Kim WT (2004) The Vr-PLC3 gene encodes a putative plasma membrane-localized phosphoinositide-specific phospholipase C whose expression is induced by abiotic stress in mung bean (Vigna radiata L.). FEBS Lett 556:127–136. doi: 10.1016/S0014-5793(03)01388-7 PubMedCrossRefGoogle Scholar
- Parre E, Ghars MA, Leprince A-S, Thiery L, Lefebvre D, Bordenave M, Richard L, Mazars C, Abdelly C, Savoure A (2007) Calcium signaling via phospholipase C is essential for proline accumulation upon ionic but not nonionic hyperosmotic stresses in Arabidopsis. Plant Physiol 144:503–512. doi: 10.1104/pp.106.095281 PubMedCrossRefGoogle Scholar
- Takahashi S, Katagiri T, Hirayama T, Yamaguchi-Shinozaki K, Shinozaki K (2001) Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in Arabidopsis cell culture. Plant Cell Physiol 42:214–222. doi: 10.1093/pcp/pce028 PubMedCrossRefGoogle Scholar
- Vergnolle C, Vaultier MN, Taconnat L, Renou JP, Kader JC, Zachowski A, Ruelland E (2005) The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol 139:1217–1233. doi: 10.1104/pp.105.068171 PubMedCrossRefGoogle Scholar
- Wang X, Dong J, Liu Y, Gao H (2010) A novel dehydration-responsive element-binding protein from Caragana korshinskii is involved in the response to multiple abiotic stresses and enhances stress tolerance in transgenic tobacco. Plant Mol Biol Rep 28:664–675. doi: 10.1007/s11105-010-0196-y CrossRefGoogle Scholar