Arabidopsis AtNek2 Kinase is Essential and Associates with Microtubules
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NIMA-related kinases (Neks) are a large family of serine/threonine kinases that have been linked to cell-cycle regulation in fungi and mammals. Large families of NIMA-related kinases are also conserved in plants. We demonstrate that AtNek2, a member of the NIMA-related kinase family in Arabidopsis, is a gene fundamental for plant survival and its down-regulation has a pleiotropic effect on leaf cell morphogenesis and plant development. Intracellular localization of YFP::AtNek2 showed that AtNek2 proteins co-distribute with the microtubular cytoskeleton. As a microtubular-associated protein AtNek2 might influence the dynamics of microtubules and consequently cell morphogenesis. This is supported by the observation that misexpression of AtNek2 in RNAi mutants leads to a distorted organization of cells.
KeywordsNIMA kinase Microtubules Histone H3 Cell morphogenesis
We are grateful to O. Weiß und K. Kumke for technical assistance. This work was supported by the Land Sachsen-Anhalt and the Deutsche Forschungsgemeinschaft (DFG, SFB 648).
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