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Cloning and Expression of an Anther-Abundant Polygalacturonase Gene BcMF17 from Brassica Campestris ssp. Chinensis

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Abstract

A polygalacturonase gene, designated BcMF17 (Brassica campestris Male Fertility 17), has been cloned from Brassica campastris. This gene, encoding polygalacturonase (PG), consists of a 1,194 bp open reading frame along with four exons and three introns, and exhibits 79% similarities to BcMF9 (Huang et al. 2009). Detailed multiple amino acid sequence alignment and phylogenetic tree analysis indicate that BcMF17 belongs to a pollen clade and may be involved in pollen wall formation. Multiple alignments against homologous sequences from the genus Brassica indicate that BcMF17 is highly conserved in this genus. Quantitative real-time PCR results indicate that transcripts of BcMF17 are preferentially expressed in reproductive tissues, especially abundant in male floral tissues, but are expressed at low level in vegetative tissues. BcMF17 transcripts in male floral tissues persist from the anther primordia stage till to the mature pollen stage. In situ hybridized signals of BcMF17 are highly detected during anther development. During anther development, expression patterns of BcMF17 are very similar to those of BcMF9; however, unlike BcMF9, BcMF17 is continuously expressed during this period, and its transcripts are detected in the whole plant during its life cycle.

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Abbreviations

BcMF17 :

Brassica campestris Male Fertility 17

BcMF9 :

Brassica campestris Male Fertility 9

qRT-PCR:

quantitative Real-time polymerase chain reaction

PG:

Polygalacturonase

cDNA-AFLP:

cDNA–amplified fragment length polymorphism

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Acknowledgments

This work was supported by the Chinese Natural Science Foundation (No. 31071805).

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Authors and Affiliations

  1. Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310029, China

    Aihong Zhang, Qizhen Chen, Li Huang, Lin Qiu & Jiashu Cao

  2. Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310029, People’s Republic of China

    Jiashu Cao

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  1. Aihong Zhang
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  2. Qizhen Chen
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  3. Li Huang
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  4. Lin Qiu
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Correspondence to Jiashu Cao.

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Zhang, A., Chen, Q., Huang, L. et al. Cloning and Expression of an Anther-Abundant Polygalacturonase Gene BcMF17 from Brassica Campestris ssp. Chinensis . Plant Mol Biol Rep 29, 943–951 (2011). https://doi.org/10.1007/s11105-011-0298-1

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