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Plant Molecular Biology Reporter

, Volume 29, Issue 3, pp 702–713 | Cite as

Fine Mapping of qHD4-1, a QTL Controlling the Heading Date, to a 20.7-kb DNA Fragment in Rice (Oryza sativa L.)

  • Binbin Wang
  • Changxiang Zhu
  • Xu Liu
  • Wenying Wang
  • Hanfeng Ding
  • Mingsong Jiang
  • Guangxian Li
  • Wei Liu
  • Fangyin Yao
Article

Abstract

A library consisting of 1,123 single-segment substitution lines (SSSLs) in the same genetic background of an elite rice variety Huajingxian74 (HJX74) was evaluated for heading date. From this library, the SSSL W05-1-11-5-16-2-5 with the substituted interval of PSM103—RM348-OSR15-PSM382-RM131-RM127—RM280 was found having a gene, which stably performed extreme late heading date which performed stable and late heading in the different environments of Shandong, Guangdong, and Hainan. To map the gene governing heading date, the SSSL W05-1-11-5-16-2-5 was crossed with the recipient HJX74 to develop an F2 segregating population. The distribution of late and early heading plants in this population fitted a segregation ratio of 3:1, indicating the late heading was controlled by a dominant gene. The gene locus for heading date was tentatively designated as qHD4-1. Using a random sample of 460 individuals from the F2 segregation population, the qHD4-1 locus was mapped between two SSR markers RM3335 and RM17572, with genetic distances of 0.1 and 0.2 cM, respectively. For fine mapping of qHD4-1, a large F2:3 segregating population of 3,000 individuals were developed from F2 plants heterozygous in the RM3335-RM17572 region. Recombinants analyses further mapped qHD4-1 to an interval of 20.7-kb-bounded WB05 and the WB06. Sequence analysis of this 20.7-kb region revealed that it contains three open reading frames (ORFs), encoding wall-associated receptor kinase-like 5, putative F-box domain containing protein, and putative arogenate/prephenate dehydratase. Of them, ORF1, predicting to encode serine/threonine kinase, is considered the most likely as the candidate gene. The genetic and physical map of the qHD4-1 locus will be very useful in molecular cloning of the qHD4-1gene.

Keywords

Oryza sativa Heading date Single-segment substitution line (SSSL) Physical mapping Sequence information 

Notes

Acknowledgements

We are grateful to professor Zhang Guiquan, South China Agricultural University, Guangzhou for kindly providing us with the SSSL populations. This work was financially supported by the National High-Tech “863” Program of P.R. China (No. 2006AA100101), China Science and Technology Support Program (No. 2006BAD13B01-18), the High-Tech Innovation Foundation of Shandong Academy of Agricultural Science (No. 2007YCX007), the Open Foundation of the State Key Laboratory of Crop Biology, China (2009KF05, 2010KF07), and the National Program on Research and the Development of Transgenic Plants (No. 2008ZX08001-001, 2009ZX08001-010B)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Binbin Wang
    • 1
  • Changxiang Zhu
    • 3
  • Xu Liu
    • 1
  • Wenying Wang
    • 1
  • Hanfeng Ding
    • 1
  • Mingsong Jiang
    • 2
  • Guangxian Li
    • 2
  • Wei Liu
    • 1
  • Fangyin Yao
    • 1
  1. 1.High-Tech Research CenterShandong Academy of Agricultural ScienceJinanPeople’s Republic of China
  2. 2.Shandong Rice Research InstituteJiningPeople’s Republic of China
  3. 3.State Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anPeople’s Republic of China

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