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Plant Molecular Biology Reporter

, Volume 29, Issue 3, pp 693–701 | Cite as

Evaluation of Genetic Diversity in Lemons and Some of Their Relatives Based on SRAP and SSR Markers

  • Aydin Uzun
  • Turgut Yesiloglu
  • Ilknur Polat
  • Yildiz Aka-Kacar
  • Osman Gulsen
  • Bilge Yildirim
  • Onder Tuzcu
  • Seyla Tepe
  • Ihsan Canan
  • Sahin Anil
Article

Abstract

Genetic diversity was evaluated by sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers among 45 lemons (Citrus limon (L.) Burm. f.), five citrons (Citrus medica L.), four rough lemons (Citrus jambhiri Lush), and two Citrus volkameriana accessions. Twenty-one SRAP primer combinations produced a total of 141 (77%) polymorphic fragments with an average of 6.7 fragments per primer combinations whereas 13 SSR primers produced a total of 26 (76%) polymorphic fragments with an average of 2.0 per primer. The unweighted pair-group method arithmetic average analysis as assessed with combined SRAP and SSR data demonstrated that the accessions had a similarity range from 0.65 to 1.00. Rough lemons and C. volkameriana accessions were relatively closely related. In lemon group, accessions from hybrid origin were distant from the others. We also applied principal components analysis (PCA) for a better presentation of relation among the accessions studies. Using PCA, 88.7% of the total variation in the original dimensions could be represented by just the two dimensions defined by the first two PCs. Although nearly all accessions could be distinguished, there was a low level of genetic diversity detected among lemon cultivars.

Keywords

Citrus Citrus limon Lemon relatives Molecular characterization 

Notes

Acknowledgements

The authors thanks to Scientific and Technological Research Council (TUBITAK), General Directorate of Agricultural Research of Ministry of Agriculture and Rural Affairs, and Scientific Research Project Unit of University of Cukurova, Adana, Turkey for funding and supporting.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Aydin Uzun
    • 1
  • Turgut Yesiloglu
    • 2
  • Ilknur Polat
    • 3
  • Yildiz Aka-Kacar
    • 2
  • Osman Gulsen
    • 1
  • Bilge Yildirim
    • 2
  • Onder Tuzcu
    • 2
  • Seyla Tepe
    • 3
  • Ihsan Canan
    • 4
  • Sahin Anil
    • 5
  1. 1.Department of HorticultureErciyes UniversityKayseriTurkey
  2. 2.Department of HorticultureCukurova UniversityAdanaTurkey
  3. 3.West Mediterranean Agricultural Research InstitueAntalyaTurkey
  4. 4.Alata Horticultural Research InstituteErdemliTurkey
  5. 5.General Directorate of Agricultural Research of Ministry of Agriculture and Rural AffairsAnkaraTurkey

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