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Plant Molecular Biology Reporter

, Volume 29, Issue 3, pp 554–562 | Cite as

Cultivar Identification and Genetic Diversity of Chinese Bayberry (Myrica rubra) Accessions Based on Fluorescent SSR Markers

  • Rang-Jin Xie
  • Jian Zhou
  • Guo-Yun Wang
  • Shui-Ming Zhang
  • Lin Chen
  • Zhong-Shan Gao
Article

Abstracts

A collection of 122 Chinese bayberry accessions and one wax myrtle (Myrica cerifera L.) were analyzed with 14 polymorphic simple sequence repeats (SSRs). The average number of alleles per locus was 9.3, and polymorphism information content varied from 0.07 to 0.83, with a mean value of 0.62. The genetic relationships among the 123 accessions were analyzed using the unweighted pair-group method with arithmetic mean (UPGMA). The similarity among all the accessions, based on Dice’s coefficient, varied from 0.78 to 0.99, and 0.74 between the Chinese bayberries and wax myrtle. A set of 122 Chinese bayberries clustered into four groups, with the first group further divided into six subgroups. The accessions originating from the same geographical region were more closely related than those from different regions, although extensive gene flow has taken place. The Mantel test, used to compare similarity matrices calculated from AFLP and SSR data, showed that their combination could provide information on the genetic relationship among the Chinese bayberry accessions. Ten selected SSR markers were able to distinguish most accessions, and multiplex PCR systems were developed. In addition, we found that SSRs developed from Chinese bayberry are transferable to M. cerifera.

Keywords

Simple sequence repeats Chinese bayberry Cultivar identification Transferability 

Notes

Acknowledgements

This research was supported by the Science and Technology Project of Zhejiang Province (2006 C14016 and 2009R50033), and the Special Research Fund for Public Welfare in Chinese Agriculture (contract no. 200903044).

Supplementary material

11105_2010_261_MOESM1_ESM.doc (176 kb)
Table 1 List of 122 bayberry accessions and one M. cerifera included in the study (DOC 176 kb)
11105_2010_261_MOESM2_ESM.ppt (824 kb)
Fig. 1 Map of China with the indication of the location where the different cultivars were collected (PPT 824 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Rang-Jin Xie
    • 1
    • 2
  • Jian Zhou
    • 1
  • Guo-Yun Wang
    • 3
  • Shui-Ming Zhang
    • 4
  • Lin Chen
    • 1
  • Zhong-Shan Gao
    • 1
  1. 1.Department of Horticulture, Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, State Agriculture MinistryZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Citrus Research InstituteSouthwest UniversityChongqingPeople’s Republic of China
  3. 3.Fruit Research InstituteYuyao NingboPeople’s Republic of China
  4. 4.Department of HorticultureAnhui Agricultural UniversityHefeiPeople’s Republic of China

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