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Plant Molecular Biology Reporter

, Volume 29, Issue 2, pp 424–430 | Cite as

Ovexpression of a Vacuolar H+-ATPase c Subunit Gene Mediates Physiological Changes Leading to Enhanced Salt Tolerance in Transgenic Tobacco

  • Chenxi Xu
  • Lei Zheng
  • Caiqiu Gao
  • Chao Wang
  • Guifeng Liu
  • Jing Jiang
  • Yucheng Wang
Article

Abstract

H+-ATPase subunit c (VHA-c) is involved in the adaptation to environmental stresses, including salt, drought, and heavy metals. However, it remains unclear whether VHA-c can induce a physiological response related to stress tolerance. To investigate this possibility, we generated transgenic tobacco lines overexpressing a V-ATPase subunit c (LbVHA-c1) gene from Limonium bicolor (Bunge) Kuntze. Compared with wild-type (WT) tobacco, superoxide dismutase (SOD) and peroxidase (POD) activities in the transgenic plants were significantly enhanced under salt stress conditions. The level of malondialdehyde (MDA) in the transgenic plants was significantly lower than that in WT plants grown under salt stress conditions. Moreover, the transgenic plants displayed obviously better growth than the WT plants under salt stress. These results suggest that LbVHA-c1 may confer stress tolerance through enhancing POD and SOD activities, and by protecting membranes from damage by decreasing lipid peroxidation under salt stress.

Keywords

H+-ATPase subunit c Salt stress Limonium bicolor 

Notes

Acknowledgments

This work was supported by Fundamental Research Funds for the Central Universities (DL09DA01), the National Natural Science Foundation of China (No. 30972387), Genetically modified organisms breeding major projects (2009ZX08009-098B), and National High Technology Research and Development Program of China (863 Program) (2009AA10Z107).

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Chenxi Xu
    • 1
  • Lei Zheng
    • 1
  • Caiqiu Gao
    • 1
  • Chao Wang
    • 1
  • Guifeng Liu
    • 1
  • Jing Jiang
    • 1
  • Yucheng Wang
    • 1
  1. 1.Key Laboratory of Forest Tree Genetic improvement and Biotechnology, Ministry of EducationNortheast Forestry UniversityHarbinChina

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