Transferability and Level of Heterozygosity of Microsatellite Markers in Citrus Species
Microsatellite markers are a powerful tool for genetic studies, including germplasm conservation, cultivar identification, and integration of linkage maps. Several works have shown that primer pairs designed for one species can be used in related species to facilitate wider application because it reduces the costs for primer development. The objective of this study was to evaluate the transferability of microsatellite primers which was previously developed from the genomic library of Pêra sweet orange (Citrus sinensis L. Osbeck) and to determine the level of heterozygosity between citrus accessions and related genera. Twenty-four microsatellite loci were evaluated on 12 genotypes of Citrus, Poncirus, and an intergeneric hybrid. All analyzed markers were transferable across all genotypes. Seventeen loci were polymorphic, and the number of alleles per loci ranged from one to six. The lowest level of heterozygosity was observed for Poncirus trifoliata (L.) Raf. cultivars while the highest level was for Swingle citrumelo. In general, microsatellite markers showed wide genetic variation and demonstrated that they can be useful in citrus breeding programs.
KeywordsAllelic diversity Expressed sequence Mapping Simple sequence repeats SSR-ESTs
This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo. MCY, VMN, and MAM are recipients of research fellowships from CNPq.
- Cristofani M, Machado MA, Novelli VM, Souza AA, Targon MLPN (2000) Construction of linkage maps of Poncirus trifoliata and Citrus sunki based on microsatellite markers. In: Proc 9th International Society of Citriculture Congress, Orlando, USA, December, 2000, pp 175–178Google Scholar
- Hodgson RW (1967) Horticultural varieties of citrus. In: Reuther W, Webber HJ, Batchelor LD (eds) The citrus industry. Berkeley, University of California, pp 431–589Google Scholar
- Luro FL, Costantino G, Terol J, Argout X, Allario T, Wincker P, Talon M, Ollitrault P, Morillon R (2008) Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping. BMC Genomics 9:287. doi: 10.1186/1471-2164-9-287 PubMedCrossRefGoogle Scholar
- Rafalski JA, Vogel JM, Morgante M, Powell W, Andre C, Tingey SV (1996) Generating and using DNA markers in plants. In: Birren B, Lai E (eds) Analysis of non-mammalian genomes—a practical guide. Academic, New York, pp 75–134Google Scholar
- Rohlf FJ (1993) NTSYS-pc, numerical taxonomy and multivariate analysis system, version 2.02g. Exeter Software, SetauketGoogle Scholar
- Swingle WT, Reece PC (1967) The botany of Citrus and its wild relatives. In: Reuther W, Webber HJ, Batchelor LD (eds) The citrus industry. Berkeley, University of California, pp 190–422Google Scholar