Plant Molecular Biology Reporter

, Volume 28, Issue 2, pp 317–323 | Cite as

Characterizing Polygala L. (Polygalaceae) Species in Southern Brazil Using ISSR

  • Raquel Lüdtke
  • Gustavo Agostini
  • Silvia T. Sfoggia Miotto
  • Tatiana T. Souza-Chies


The genus Polygala is one of the seven Polygalaceae genera that occur in the Brazilian flora, covering approximately 110 species. During the taxonomic review of Polygalaceae in Southern Brazil, difficulties were found when classifying species with very similar morphology, and morphological data alone could not clarify these interspecific relationships. In this context, inter-simple sequence repeat (ISSR) molecular markers were used in an attempt to characterize the genetic diversity and relationships among Polygala species. Nine Polygala species were analyzed using six selected ISSR primers that generated a total of 75 bands (100% polymorphic). The relationships were evaluated by dendrograms using the unweighted pair group method using arithmetic averages algorithm. The use of ISSR to solve the taxonomic problems was very useful for the Brazilian Polygala species. This is the first report of a molecular characterization of the Brazilian Polygala species to successfully group the different species. The ISSR results are in agreement with the morphological evidence of a new Polygala species from Southern Brazil.


Interspecific relationships ISSR Polygala Polygalaceae Southern Brazil 



The authors thank Coordenação de Aperfeiçoamento de Pessoal e Nível Superior by the fellowship of the first author and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for financial support of this study. The authors also thank Felix J. Nonnenmacher for English revision.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Raquel Lüdtke
    • 1
    • 2
  • Gustavo Agostini
    • 1
  • Silvia T. Sfoggia Miotto
    • 1
    • 3
  • Tatiana T. Souza-Chies
    • 1
    • 3
  1. 1.Programa de Pós-Graduação em BotânicaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Departamento de Botânica, Instituto de BiologiaUniversidade Federal de Pelotas—UFPelPelotasBrazil
  3. 3.Departamento de BotânicaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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