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Plant Molecular Biology Reporter

, Volume 27, Issue 4, pp 488–495 | Cite as

A Comparative Analysis of ISSR and RAPD Markers for Study of Genetic Diversity in Shisham (Dalbergia sissoo)

  • Mohd Arif
  • N. W. Zaidi
  • Y. P. Singh
  • Qazi Mohd Rizwanul Haq
  • U. S. Singh
Article

Abstract

Shisham (Dalbergia sissoo) is one of the most preferred timber tree species of South Asia. Two DNA-based molecular marker techniques, intersimple sequence repeat (ISSR) and random amplified polymorphism DNA (RAPD), were compared to study the genetic diversity in this species. A total of 30 polymorphic primers (15 ISSR and 15 random) were used. Amplification of genomic DNA of 22 genotypes, using ISSR analysis, yielded 117 fragments, of which 64 were polymorphic. Number of amplified fragments with ISSR primers ranged from five to ten and varied in size from 180 to 1,900 bp. Percentage polymorphism ranged from 0 to 87.5. The 15 RAPD primers produced 144 bands across 22 genotypes, of which 84 were polymorphic. The number of amplified bands varied from five to 13, with size range from 180 to 2,400 bp. Percentage polymorphism ranged from 0 to 100, with an average of 58.3 across. RAPD markers were relatively more efficient than the ISSR assay. The mental test between two Jaccard’s similarity matrices gave r ≥ 0.90, showing very good fit correlation in between ISSR- and RAPD-based similarities. Clustering of isolates remained more or less the same in RAPD and combined data of RAPD and ISSR. The similarity coefficient ranged from 0.734 to 0.939, 0.563 to 0.946, and 0.648 to 0.920 with ISSR, RAPD, and combined dendrogram, respectively.

Keywords

Dalbergia sissoo Genetic variability ISSR Molecular marker RAPD 

Notes

Acknowledgements

The authors are thankful to Dr. Ombir Singh, Division of Silviculture, Forest Research Institute, Dehradun, for providing the seed sources of different germplasms and to the Department of Biotechnology, Government of India, for providing financial support for conducting the investigation and fellowship to the first author.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Mohd Arif
    • 1
  • N. W. Zaidi
    • 1
  • Y. P. Singh
    • 2
  • Qazi Mohd Rizwanul Haq
    • 3
  • U. S. Singh
    • 1
    • 4
  1. 1.Department of Plant PathologyG. B. Pant University of Agriculture and TechnologyPantnagarIndia
  2. 2.Forest Pathology DepartmentForest Research InstituteDehradunIndia
  3. 3.Department of BiosciencesJamia Millia IslamiaNew DelhiIndia
  4. 4.South Asia Regional Project CoordinatorInternational Rice Research Institute, IRRI-India Office, NASC ComplexNew DelhiIndia

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