Stable isotope tracer experiments provide a powerful tool for understanding plant root distributions, resource uptake, niche partitioning and water cycling. Plant water is typically collected from pre-transpiring tissues to avoid the effects of evaporative isotope enrichment at the leaf surface, but extracting water from these plant samples is difficult and expensive. The purpose of this study was to test a simple transpiration bagging approach for measuring hydrologic tracer uptake.
Sampling was performed as part of a depth-specific tracer experiment in which 2H2O was injected to target depths (5, 15, 30, 60, or 150 cm) in different replicated plots. One day following injections, leaves from three species were sealed in bags for 16 h and transpired water was collected. Water from pre-transpiring stem tissue was then collected in a separate set of samples and extracted using cryogenic distillation.
Deuterium concentrations from the two techniques were correlated (R2 = 0.84) and both approaches produced similar descriptions of vertical root distributions for three dominant plant species. 18O concentrations from the two techniques were not correlated.
Bagging transpired water produced similar estimates of 2H tracer uptake as the standard sampling technique. Bagging requires no destructive sampling, specialized laboratory equipment, training or consumables and is expected to halve sampling costs. While effective in this tracer experiment, bagging may be not be effective in natural abundance experiments, or tracer experiments with very small plants or small transpiration rates (i.e., early-season or arid sites).
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Thanks to C. Carlisle, C. Pint, J. Allenbrand, and M. Holdrege for assistance with field work. K. Foley, S. Rawlings, K. Slabaugh, A. Yamaguchi, A. Zlevor, and A. Brookes assisted with sample preparation. This work was supported by grants from the US National Science Foundation Long-Term Ecological Research Program (LTER) including DEB-0620652 and DEB-1234162. Further support was provided by the Cedar Creek Ecosystem Science Reserve and the University of Minnesota. This research was also supported by the Utah Experiment Station and approved as journal paper number 9403.
Research was supported by a grant from NSF DEB, award #1354129. This research was also supported by the Utah Experiment Station and approved as journal paper number 9403.
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Kulmatiski, A., Forero, L.E. Bagging: a cheaper, faster, non-destructive transpiration water sampling method for tracer studies. Plant Soil (2021). https://doi.org/10.1007/s11104-021-04844-w
- Stable isotope
- Root distribution
- Water uptake