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Differential response of Phytophthora sojae zoospores to soybean seed exudates provides evidence of seed exudates participate in host resistance

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Abstract

Aims

Differential effects of the seed exudates of susceptible and resistant soybean cultivars Sloan and Williams 82 on the zoospores of soilborne pathogenic Phytophthora sojae are not clear. This study aims to elucidate the role of the seed exudates in the host resistance of soybean against P. sojae during prior to infection.

Methods

The response behaviors of P. sojae zoospores to the seed exudates were performed in an assay chamber and concave slide, the proteomes of P. sojae zoospores treated with the seed exudates were analyzed by TMT method. The expression of genes encoding key proteins was quantified by qRT-PCR.

Results

The zoospores of P. sojae exhibited significant chemotaxis to the both seed exudates and a stronger attracted was found to the seed exudates of Sloan. The seed exudates of Sloan also had a stonger promotion than that of Williams 82 on encystment of zoospores. During the responding process, the key proteins in zoospores related to chemotaxis were upregulated, the upregulation fold of these proteins in responding to Sloan was higher than that of Williams 82. Proteins differentially expressed promote the encystment of zoospores. The changes in expression of these proteins was several fold higher in the zoosprores exposed to the seed exudates of Sloan than that of Williams 82.

Conclusions

Evidence of differential changes in protein expression in P. sojae zoospores exposed to the seed exudates of both soybean supports the differential developmental behaviors such as chemotaxis and encystment of the zoospores in responding to the two seed exudates.

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Abbreviations

MAPK:

Mitogen-activated protein kinase

qRT-PCR:

quantitative Real-time polymerase chain reaction

UPLC:

Ultra performance liquid chromatography

LC-MS/MS:

Liquid chromatography-mass spectrometry/mass spectrometry

Gα:

G protein α subunit

GPCR:

G protein-coupled receptor

TCA:

Trichloroacetic acid

TEAB:

Tetraethylammonium bromide

NSI:

Nanoelectrospray ionization

TMT:

Tandem mass tag

HPLC:

High performance liquid chromatography

NCE:

Normalized collision energy

AGC:

Automatic gain control

Gβ :

G protein β subunit

GO:

Gene Ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

cv.:

Cultivar

PI3K:

Phosphatidylinositol 3-kinase

PI4K:

Phosphatidylinositol 4-kinase

PKC:

Protein kinase C

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Acknowledgements

Thanks for the technical support and bioinformatics analysis of PTB BioLabs.

Funding

This research was supported by the National Natural Science Foundation of China (Grant No. 31670444; 31370449) to Jingzhi Wen.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Zhuoqun Zhang, Haixu Liu, Xiangqi Bi, Han Yu, Ying Xu, Yufei Chen, Zhiyue Yang & Jingzhi Wen

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  1. Zhuoqun Zhang
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  2. Haixu Liu
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  3. Xiangqi Bi
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  4. Han Yu
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  5. Ying Xu
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  6. Yufei Chen
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  7. Zhiyue Yang
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  8. Jingzhi Wen
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were mainly performed by Zhuoqun Zhang, Haixu Liu, Xiangqi Bi, Han Yu, Ying Xu, Yufei Chen, and Zhiyue Yang. The first draft of the manuscript was written by Zhuoqun Zhang with help from Jingzhi Wen, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jingzhi Wen.

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Zhang, Z., Liu, H., Bi, X. et al. Differential response of Phytophthora sojae zoospores to soybean seed exudates provides evidence of seed exudates participate in host resistance. Plant Soil 452, 601–614 (2020). https://doi.org/10.1007/s11104-020-04607-z

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