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Rhizobium sophorae, Rhizobium laguerreae, and two novel Rhizobium genospecies associated with Vicia sativa L. in Northwest China

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Abstract

Aims

To identify the rhizobia nodulating Vicia sativa in Northwestern China and to estimate their geographic distribution.

Methods

Rhizobia trapped with V. sativa plants from soils at six sites in the northwest of China were classified into genotypes by PCR-based restriction fragment length polymorphism (RFLP) of 16S–23S rRNA intergenic spacer (IGS) and 16S rRNA genes, and phylogenetic analyses of housekeeping (16S rRNA, recA, atpD) and symbiotic genes were performed for the representative strains. Soil physicochemical characteristics were recorded and canonical correlation analysis was performed to examine the correlations between soil features and distribution of rhizobial genotypes.

Results

A total of 202 rhizobial isolates were discriminated by RFLP analyses into 15 IGS types and a single 16S rRNA type, which were identified as Rhizobium by 16S rRNA gene phylogeny and as four clusters by multilocus sequence analysis (MLSA). Cluster 1 covering 86 strains and 7 IGS types prevalent in all sites was identified as Rhizobium laguerreae; cluster 2 was R. sophorae with 18 strains in 2 IGS types found in the site Q-GD. Each of cluster 3 and cluster 4 contained three IGS types representing two novel Rhizobium genospecies specific to Gansu Province and Shanxi Province, respectively. Four nodC phylogenetic clades were defined among the isolates.

Conclusions

R. sophorae, R. laguerreae, and two novel Rhizobium genospecies with diverse symbiotic genotypes are associated with Vicia sativa L. in Northwest China. Their biogeographic patterns are mainly directed by soil pH and salinity. This is the first study on the diversity of rhizobia nodulating Vicia sativa in China.

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Funding

This work was financed by China Agriculture Research System – Green Manure (Project No. CARS-22), the National Natural Science Foundation of China (Project No. 31400008), and National Human and Social Security Ministry of Science and Technology for Overseas Returnees (Project No. 21104000005). ETW is financially supported by grants SIP20171259 authorized by Instituto Politécnico Nacional, Mexico.

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Authors and Affiliations

  1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, 450000, Henan Province, People’s Republic of China

    Junjie Zhang, Yimin Shang, Shanshan Peng & Chen Guo

  2. Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou, 450000, Henan Province, People’s Republic of China

    Junjie Zhang

  3. Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou, 450002, Henan Province, People’s Republic of China

    Junjie Zhang

  4. State Key Laboratory of Agrobiotechnology, Beijing, 100193, China

    Wenfeng Chen

  5. College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing, 100193, People’s Republic of China

    Wenfeng Chen

  6. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, C.P. 11340, C. D. México, Mexico

    Entao Wang

  7. IRD, LSTM, CIRAD, INRA, SupAgro, Université de Montpellier, Campus de Baillarguet TA-A-82/J, 34398, Montpellier Cedex 5, France

    Philippe de Lajudie

  8. Institute of Plant Nutrition and Resource Environment, Henan Academy of Agricultural Sciences, Zhengzhou, 450000, Henan Province, People’s Republic of China

    Benyin Li & Chunzeng Liu

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  1. Junjie Zhang
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Zhang, J., Shang, Y., Peng, S. et al. Rhizobium sophorae, Rhizobium laguerreae, and two novel Rhizobium genospecies associated with Vicia sativa L. in Northwest China. Plant Soil 442, 113–126 (2019). https://doi.org/10.1007/s11104-019-04168-w

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