Enhancement of rice growth and yield in actual acid sulfate soils by potent acid-resistant Rhodopseudomonas palustris strains for producing safe rice
Purple nonsulfur bacteria (PNSB), Rhodopseudomonas palustris strains (TLS06, VNW02, VNW64 and VNS89), were investigated to increase rice growth and grain yield in acid sulfate soils (ASS) with low available phosphorus (Pavail).
P-solubilizers were tested in vitro. A 4 × 3 factorial design consisted of PNSB at 5.4 × 104 cells g−1 dry soil weight (mixture of 4 strains, VNW64 singly, or no-PNSB) and P fertilizer levels (0, 30, 45 and 60 kg P2O5 ha−1) that were used with the rice variety OM5451in pots with two types of ASS (Hon Dat and Phung Hiep).
The four PNSB mixture had the ability to dissolve insoluble P from variscite and strengite. The combination of mixed culture with 45 P was the most effective, increasing grain yield by 34%. Enhancement of rice growth and yield in both soils corresponded to the maximal levels of Pavail, total P and NH4+, and the lowest levels of Alexch and Fe2+. Soil health with this treatment was significantly improved, with a strong positive correlation between PNSB population and phosphatase activity in both soil types.
The combination of PNSB mixture with P fertilizer reduced the amount of chemical fertilizer needed for maximal grain yield, provided safe rice, and maintained soil health.
KeywordsAluminum Available phosphorus Ferrous Purple nonsulfur bacteria Soil health Soil phosphatase
The first author was supported by the Graduate School, Prince of Songkla University, and additional support was received from the Thailand’s Education Hub for Southern Region of ASEAN Countries (TEH-AC), grant number TEH-AC 027/2015.
Compliance with ethical standards
Conflict of interest
- Brown JW (2013) Enrichment and isolation of purple non-sulfur bacteria. Department of Biological Sciences. College of Sciences. North Carolina State University. http://www.mbio.ncsu.edu/mb452/purple_nonsulfurs/purples.html. Accessed 15 Nov 2017
- Cho MH, Kyaw N, Kyaw KW, Swe SM (2017) Assessment of soil indigenous nutrient supply as a natural resource management in rice production towards climate resilience agriculture. In Proceedings of the Tenth Agricultural Research Conference, Yezin Agricultural University, Nay Pyi Raw, Myanmar, 11–12 January 2017 (pp 159–177). Yezin Agricultural UniversityGoogle Scholar
- Horneck DA, Sullivan DM, Owen JS, Hart JM (2011) Soil test interpretation guide. EC 1478. Corvallis, OR: Oregon State University Extension ServiceGoogle Scholar
- IRRI, International Rice Research Institute (2014) Standard procedure for determining yield components at harvest.< https://sites.google.com/a/irri.org/oryza2000/calibration-and-validation/experimental-data-collection-and-analysis/standard-procedure-for-determining-yield-components-at-harvest>(Accessed 8 Jan 2017)
- Khalimi K, Suprapta DN, Nitta Y (2012) Effect of Pantoea agglomerans on growth promotion and yield of rice. Agric Sci Res J 2(5):240–249Google Scholar
- Khuong NQ, Kantachote D, Onthong J, Sukhoom A (2017) The potential of acid-resistant purple nonsulfur bacteria isolated from acid sulfate soils for reducing toxicity of Al3+ and Fe2+ using biosorption for agricultural application. Biocatal Agric Biotechnol 12:329–340Google Scholar
- Lakshmi KVNS (2012) Cultured diversity of purple anoxygenic phototrophic rhizobacteria of paddy and their plant growth promoting attributes (Doctoral dissertation, Jawaharlal Nehru Technological University, Hyderabad). Page:243Google Scholar
- Nunkaew T, Kantachote D, Kanzaki H, Nitoda T, Ritchie RJ (2014) Effects of 5-aminolevulinic acid (ALA)-containing supernatants from selected Rhodopseudomonas palustris strains on rice growth under NaCl stress, with mediating effects on chlorophyll, photosynthetic electron transport and antioxidative enzymes. Electron J Biotechnol 17(1):19–26CrossRefGoogle Scholar
- Sakpirom J, Kantachote D, Nunkaew T, Khan E (2017) Characterizations of purple non-sulfur bacteria isolated from paddy fields, and identification of strains with potential for plant growth-promotion, greenhouse gas mitigation and heavy metal bioremediation. Res Microbiol 168:266–275CrossRefPubMedGoogle Scholar
- Soomro AA, Abro MA, Leghari N, Leghari GM, Soomro AA (2015) Evaluation of aluminum toxicity tolerance in rice (Oryza sativa L.). Sci Int 27(3):2251–2255Google Scholar
- Sparks DL, Page AL, Helmke PA, Loeppert RH, Soltanpour PN, Tabatabai MA, Johnston CT, Sumner ME (1996) Methods of soil analysis. Part 3-Chemical methods. SSSA Book Ser. 5.3. SSSA, ASA, Madison, WI. https://doi.org/10.2136/sssabookser5.3
- Walinga I, van Vark W, Houba VJG, van der Lee JJ (1989) Plant analysis procedure, Part 7. Department of Soil Science and Plant Nutrition, Wageningen Agricultural UniversityGoogle Scholar
- Xuan VT, Matsui S (1998) Development of farming systems in the Mekong delta of Vietnam. Ho Chi Minh City Publ. House, Ho Chi Minh CityGoogle Scholar