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Plant and Soil

, Volume 434, Issue 1–2, pp 217–229 | Cite as

Metabolic changes in roots of trifoliate orange [Poncirus trifoliate (L.) Raf.] as induced by different treatments of boron deficiency and resupply

  • Xiuwen Wu
  • Guidong Liu
  • Muhammad Riaz
  • Lei Yan
  • Cuncang JiangEmail author
Regular Article

Abstract

Background and aims

Boron (B) deficiency is one of the major abiotic stresses to citrus. Trifoliate orange, as the most important citrus rootstock in China, is sensitive to B deficiency. B deficiency has serious negative effects on metabolic processes in roots, but whether the hazardous effects can be alleviated to some degree with B resupply remains unclear. The purpose of this study was to investigate the effects of B resupply on metabolites and metabolic pathway in B-starved trifoliate orange [Poncirus trifoliate (L.) Raf.] roots.

Methods

Gas chromatography-mass spectrometry (GC-MS) was used to analyze the metabolic profiles in roots under different treatments of B deprivation and B resupply.

Results

B deficiency induced an obvious enlargement of root tips and significant alterations in metabolites. Changes were also observed in basic or neutral amino acids, several organic acids and carbohydrates of B-starved roots. However, one week of B resupply quickly reversed the changes in the roots with the initiation of new roots across the inflated root tips, and the restored some of metabolites to a normal level, such as proline (Pro), linoleic acid, 6-deoxy-D-glucose and myo-inositol, indicating that these metabolites are very sensitive to B availability.

Conclusions

The data suggest that B resupply may contribute to the obvious, although not full, recovery of the root growth by reversing the central metabolite changes. This study provides a new insight into the relationship between root growth and the alteration on metabolites induced by B deficiency and B resupply, which can be expected to make an important contribution to the scientific application of B fertilizer.

Keywords

Boron deprivation Boron resupply Gas chromatography-mass spectrometry (GC-MS) Root Metabolic profiles 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (41271320) and the Fundamental Research Funds for the Central Universities (2017PY055). We would like to thank C.C.J. for supervising this study, G.D.L for interpreting the results of the study, L.Y. for renewing the nutrition solution in the experiment and determining the B concentration, M. R. for revising the manuscript in grammar.

Supplementary material

11104_2018_3684_MOESM1_ESM.docx (1 mb)
ESM 1 (DOCX 1052 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Xiuwen Wu
    • 1
  • Guidong Liu
    • 2
  • Muhammad Riaz
    • 1
  • Lei Yan
    • 1
  • Cuncang Jiang
    • 1
    Email author
  1. 1.Microelement Research Center, College of Resources and EnvironmentHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.National Navel Orange Engineering Research CenterGannan Normal UniversityGanzhouPeople’s Republic of China

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