Unravelling community assemblages through multi-element stoichiometry in plant leaves and roots across primary successional stages in a glacier retreat area
Background and aims
Our understandings on the patterns and mechanisms of plant community assembly during succession, especially the primary succession in glacier retreat areas, remain limited. The Hailuogou Glacier Chronosequence provides a distinctive place to disentangle the biotic interactions and abiotic filtering effects on community successional trajectories.
Through community-weighted approaches, we quantified elements allocation and regulation in leaves and roots, N:P stoichiometry, and the biotic and abiotic controls guiding community dynamics along the 120-year chronosequence.
Across seven primary successional stages, plant leaves featured higher concentrations of macro-elements with lower coefficients of variation (CV) with increasing succession; whereas, fine roots contained more micro-elements with higher CV. From the early to late stages, foliar N:P increased linearly from 8.2 to 20.1.
These findings highlighted that the limiting factor for plant growth shifted from N to P over one century of deglaciation. Edaphic factors (pH, bulk density, N and P concentrations) acted as deterministic filtering for trait convergence in the early stages, while biotic factors (species richness and plant litter biomass) for competitive exclusion dominated the late stages hosting species with stronger homoeostatic regulation and more conservative nutrient use.
KeywordsEdaphic and biotic drivers Hailuogou Glacier Chronosequence Elements homoeostatic regulation Plant community assembly
The authors are grateful to the Gongga Mountain Alpine Ecosystem Observation Station, Chinese Academy of Sciences for logistic support. This work was supported by the National Science Foundation of China (Nos. 31570598 and 31370607), the Talent Program of Hangzhou Normal University (2016QDL020) and the Frontier Science Key Research Programs of Chinese Academy of Sciences (QYZDB-SSW-DQC037). The authors also thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.
- Achat DL, Bakker MR, Zeller B, Pellerin S, Bienaimé S, Morel C (2010) Long-term organic phosphorus mineralization in Spodosols under forests and its relation to carbon and nitrogen mineralization. Soil Biol Biochem 42(9):1479–1490. https://doi.org/10.1016/j.soilbio.2010.05.020 CrossRefGoogle Scholar
- Aerts R, Chapin FS (2000) The mineral nutrition of wild plants revisited: a re-evaluation of processes and patterns. In: Fitter AH, Raffaelli DG (eds) Advances in ecological research, 30, 1–67. https://doi.org/10.1016/S0065-2504(08)60016-1
- Arroyo-Rodríguez V, Melo FPL, Martinez-Ramos M, Bongers F, Chazdon RL, Meave JA, Norden N, Santos BA, Leal IR, Tabarelli M (2017) Multiple successional pathways in human-modified tropical landscapes: new insights from forest succession, forest fragmentation and landscape ecology research. Biol Rev 92:326–340. https://doi.org/10.1111/brv.12231 CrossRefPubMedGoogle Scholar
- Callaway RM, Brooker RW, Choler P, Kikvidze Z, Lortie CJ, Michalet R, Paolini L, Pugnaire FI, Newingham B, Aschehoug ET, Armas C, Kikodze D, Cook BJ (2002) Positive interactions among alpine plants increase with stress. Nature 417:844–848. https://doi.org/10.1038/nature00812 CrossRefPubMedGoogle Scholar
- Elser JJ, Bracken MES, Cleland EE, Gruner DS, Harpole WS, Hillebrand H, Ngai JT, Seabloom EW, Shurin JB, Smith JE (2007) Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems. Ecol Lett 10:1135–1142. https://doi.org/10.1111/j.1461-0248.2007.01113.x CrossRefPubMedGoogle Scholar
- Hooper D, Coughlan J, Mullen M (2008) Structural equation modelling: guidelines for determining model fit. Electron J Bus Res Methods 6:53–60Google Scholar
- Jiang Y, Lei Y, Yang Y, Korpelainen H, Niinemets Ü, Li C (2018) Divergent assemblage patterns and driving forces for bacterial and fungal communities along a glacier forefield chronosequence. Soil Biol Biochem 118:207–216. https://doi.org/10.1016/j.soilbio.2017.12.019
- Lei Y, Zhou J, Xiao H, Duan B, Wu Y, Korpelainen H, Li C (2015) Soil nematode assemblages as bioindicators of primary succession along a 120-year-old chronosequence on the Hailuogou Glacier forefield, SW China. Soil Biol Biochem 88:362–371. https://doi.org/10.1016/j.soilbio.2015.06.013 CrossRefGoogle Scholar
- Nelson DW, Sommers LE (1982) Total carbon, organic carbon and organic matter. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis. American Society of Agronomy, Madison, pp 539–579Google Scholar
- Oksanen J, Blanchet FG, Friendly M, Kindt R, Legendre P, McGlinn D, Minchin PR, O’Hara RB, Simpson GL, Solymos PS, Stevens MH, Szoecs E, Wagner H (2016) Package ‘vegan’. http://vegan.r-forge.r-project.org/
- Ostonen I, Truu M, Helmisaari H, Lukac M, Borken W, Vanguelova E, Godbold DL, Lohmus K, Zang U, Tedersoo L, Preem JK, Rosenvald K, Aosaar J, Armolaitis K, Frey J, Kabral N, Kukumägi M, Leppälammi-Kujansuu J, Lindroos AJ, Merilä P, Napa U, Njöd P, Parts K, Uri V, Varik M, Truu J (2017) Adaptive root foraging strategies along a boreal–temperate forest gradient. New Phytol 215:977–991. https://doi.org/10.1111/nph.14643 CrossRefPubMedGoogle Scholar
- Pavoine S, Vela E, Gachet S, de Belair G, Bonsall MB (2011) Linking patterns in phylogeny, traits, abiotic variables and space: a novel approach to linking environmental filtering and plant community assembly. J Ecol 99:165–175. https://doi.org/10.1111/j.1365-2745.2010.01743.x CrossRefGoogle Scholar
- Peñuelas J, Sardans J, Llusia Owen S, Carnicer J, Giambelluca TW, Rezende EL, Waite M, Niinemets Ü (2010) Faster returns on leaf economics and different biogeochemical niche in invasive compared with native plant species. Glob Chang Biol 16:2171–2185. https://doi.org/10.1111/j.1365-2486.2009.02054.x CrossRefGoogle Scholar
- R Core Team (2013) R: a language and environment for statistical computing. R foundation for statistical computing. http://www.r-project.org
- Sardans J, Janssens IA, Alonso R, Veresoglou SD, Rillig MC, Sanders TGM, Carnicer J, Filella I, Farré-Armengol G, Peñuelas J (2015) Foliar elemental composition of European forest tree species associated with evolutionary traits and present environmental and competitive conditions. Glob Ecol Biogeogr 24:240–255. https://doi.org/10.1111/geb.12253 CrossRefGoogle Scholar
- Song M, Yu L, Jiang Y, Lei Y, Korpelainen H, Niinemets Ü, Li C (2017) Nitrogen-controlled intra-and interspecific competition between Populus purdomii and Salix rehderiana drive primary succession in the Gongga Mountain glacier retreat area. Tree Physiol 37:799–814. https://doi.org/10.1093/treephys/tpx017 CrossRefPubMedGoogle Scholar
- Sperfeld E, Wagner ND, Halvorson HM, Malishev M, Raubenheimer D (2017) Bridging ecological stoichiometry and nutritional geometry with homeostasis concepts and integrative models of organism nutrition. Funct Ecol 31:286–296. https://doi.org/10.1111/1365-2435.12707
- Sterner RW, Elser JJ (2002) Ecological stoichiometry: the biology of elements from molecules to the biosphere. Princeton University Press, PrincetonGoogle Scholar
- Townsend AR, Cleveland CC, Asner GP, Bustamante MMC (2007) Controls over foliar N:P ratios in tropical rain forests. Ecology 88:107–118. https://doi.org/10.1890/0012-9658(2007)88[107,COFNRI]2.0.CO;2Google Scholar
- Zhong XH, Luo J, Wu N (1997) Researches of the forest ecosystems on Gongga Mountain. Chengdu University of Science and Technology Press, ChengduGoogle Scholar