Molecular characterization of GmSTOP1 homologs in soybean under Al and proton stress

  • Ying Zhou
  • ZhenMing Yang
  • Li Gong
  • RongKun Liu
  • HaoRan Sun
  • JiangFeng You
Regular Article
  • 41 Downloads

Abstract

Background and aims

The Sensitive to Proton Rhizotoxicity1 (STOP1) transcription factor has been implicated in the regulation of aluminium (Al) stress and proton toxicity for several plant species. This study aimed to characterize STOP1 homologs in soybean.

Method

Five GmSTOP1 homologs were studied by transcriptional expression, subcellular localization and overexpression experiments.

Results

Five GmSTOP1 homologs were nuclear-localized and exhibited transactivation activity. They constitutively expressed throughout the whole soybean plant. Their expressions were increased from 2 h, peaked at 4 h, returned to basal levels for the remaining duration of Al treatment but varied in aptitude and genotype. They were sensitive to pH conditions with various responses. Overexpression of GmSTOP1a in soybean hairy root increased the expression of the malate transporter gene GmALMT1, and decreased Al accumulation under Al stress. Its overexpression also regulated some pH-sensitive genes, including GmSTOP1c and GmCIPK23. Overexpression of GmSTOP1a in Arabidopsis slightly increase its Al resistance, and partially restored the root growth of the atstop1 mutant under Al stress.

Conclusion

GmSTOP1a contributes to both proton and Al resistance and plays a role similar to that of AtSTOP1. The functions of other four GmSTOP1 genes need further clarified.

Keywords

Aluminum toxicity Soybean Cys2His2 zinc finger protein Transcriptional regulation Proton resistance 

Abbreviations

Al

Aluminum

CaMV

Cauliflower mosaic virus

STOP1

Sensitive to Proton Rhizotoxicity1

ART1

Aluminum resistance transcription factor 1

Notes

Acknowledgements

Financial support was provided by National Natural Science Foundation of China (No. 31372124) and Natural Science Foundation of Jilin Province (20130101084JC).

Supplementary material

11104_2018_3645_MOESM1_ESM.doc (64 kb)
ESM 1 (DOC 63 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Ying Zhou
    • 1
  • ZhenMing Yang
    • 1
  • Li Gong
    • 1
  • RongKun Liu
    • 1
  • HaoRan Sun
    • 1
  • JiangFeng You
    • 1
  1. 1.Laboratory of Soil and Plant Molecular Genetics, College of Plant ScienceJilin UniversityChangchunChina

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